A5090998925- Soybean genetics and cultivation
- CRISPR and Genetic Engineering
- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Virus-based gene therapy research
- Transgenic Plants and Applications
- Genetically Modified Organisms Research
- Advanced biosensing and bioanalysis techniques
- Animal Genetics and Reproduction
- Plant and Fungal Interactions Research
- Insect-Plant Interactions and Control
- Soil Carbon and Nitrogen Dynamics
- Soil Management and Crop Yield
- RNA and protein synthesis mechanisms
- bioluminescence and chemiluminescence research
- Soil erosion and sediment transport
- Entomopathogenic Microorganisms in Pest Control
- Parvovirus B19 Infection Studies
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Phytase and its Applications
- Insect Resistance and Genetics
- Click Chemistry and Applications
- Genetics, Bioinformatics, and Biomedical Research
Brazilian Agricultural Research Corporation
2020-2025
Universidade de Brasília
2016
<title>Abstract</title> Drylands represent a significant part of the Earth's surface and include essential vulnerable ecosystems for global ecological balance. The Caatinga, with its unique biodiversity adapted to extreme conditions this semi-arid region, offers valuable opportunity expand our knowledge about these ecosystems. Here, work reveals high microbial diversity in soil rhizosphere roots presenting more specialized communities. Bacteria such as <italic>Bacilli</italic>,...
Microorganisms participate in complex interactions involving different kingdoms, so rhizosphere biodiversity mapping is essential for understanding how microbes interact with each other the soil and roots. Although microbial communities are remarkably diverse technological advances have provided a high capacity to acquire reliable sequence data, unique taxa soil, root samples remain poorly described. For first time, we organized consortium collect covering all Brazilian biomes, providing...
Recently, new serine integrases have been identified, increasing the possibility of scaling up genomic modulation tools. Here, we describe use unidirectional genetic switches to evaluate functionality six in different eukaryotic systems: HEK 293T cell lineage, bovine fibroblasts and plant protoplasts. Moreover, integrase activity was also tested human types therapeutic interest: peripheral blood mononuclear cells (PBMCs), neural stem (NSCs) undifferentiated embryonic (ES) cells. The were...
The comprehension of virus-host interactions has allowed numerous advances in developing biotechnological methodologies for plant genome editions, constituting a promising path genetic engineering. Among these advancements, phage-encoded large serine-integrases have emerged as noteworthy tools to modulate metabolic pathways by inserting, excising, or inverting DNA stretches reversible and specific way. present work shows the foundation Int-Plex@ (INTegrase PLant EXpression) binary memory...
The accelerated exploration and engineering of nucleotide sequences are directed towards quantum mechanics their intrinsic entanglements, implementing the qubits states, including development algorithms. production rate biological sequencing data has increased to approximately 1 Gb/h, but ability analyse these not kept pace due complexity issues limitations classic computing. Despite its own challenges, computing offers a potential solution for analysing extracting relevant information....
Abstract The genome of a novel group II alphabaculovirus, Perigonia lusca single nucleopolyhedrovirus (PeluSNPV), was sequenced and shown to contain 132,831 bp with 145 putative ORFs (open reading frames) at least 50 amino acids. An interesting feature this the presence nucleotide metabolism enzyme-encoding gene ( pelu112 ). predicted encode fusion thymidylate kinase tmk ) dUTP diphosphatase dut Phylogenetic analysis indicated that baculoviruses have independently acquired several times...
Tospovirus is a plant-infecting genus within the family Bunyaviridae, which also includes four animal-infecting genera: Hantavirus, Nairovirus, Phlebovirus and Orthobunyavirus. Compared to these members, structures of proteins still are poorly understood. Despite multiple studies have attempted identify candidate N protein regions involved in RNA binding multimerization for tospovirus using yeast two-hybrid systems (Y2HS) site-directed mutagenesis, ribonucleocapsids (RNPs) remains largely...
Toehold switches are biosensors useful for the detection of endogenous and environmental RNAs. They have been successfully engineered to detect virus RNAs in cell-free gene expression reactions. Their inherent sequence programmability makes engineering a fast predictable process. Despite improvements design, toehold suffer from leaky translation OFF state, which compromises fold change sensitivity biosensor. To address this, we constructed tested signal amplification circuits three triggered...
Serine integrases (Ints) are a family of site-specific recombinases (SSRs) encoded by some bacteriophages to integrate their genetic material into the genome host. Their ability rearrange DNA sequences in different ways including inversion, excision, or insertion with no help from endogenous molecular machinery, confers important biotechnological value as editing tools high host plasticity. Despite advances use prokaryotic cells, only few Ints currently used gene editors eukaryotes, partly...
Zucchini lethal chlorosis virus (ZLCV) causes significant losses in the production of cucurbits Brazil. This belongs to genus Tospovirus (family Bunyaviridae) and seems be exclusively transmitted by Frankliniella zucchini (Thysanoptera). Tospoviruses have a tripartite single-stranded RNA genome classified as S (Small), M (Medium) L (Large) RNAS. Although ZLCV was identified member 1999, its complete had not been sequenced until now. We full-length two isolates named ZLCV-SP ZLCV-DF. The...
Drylands represent a significant part of the Earth's surface and include essential vulnerable ecosystems for global ecological balance. The Caatinga, with its unique biodiversity adapted to extreme conditions this semi-arid region, offers valuable opportunity expand our knowledge about these ecosystems. Here, work reveals high microbial diversity in soil rhizosphere roots presenting more specialized communities. Bacteria such as Bacilli, Alphaproteobacteria Firmicutes excelled critical...
Soybean is a rich source of vegetal protein for both animal and human consumption. Despite the high levels in soybean seeds, industrial processing to obtain bran significantly decreases final content byproducts. To overcome this problem, cultivars with higher contents must be developed. However, selecting target proteins difficult because lack information on proteome profile bran. Therefore, study obtained comparative proteomic profiles natural coatless seeds defatted from an elite...
Abstract The authors have requested that this preprint be removed from Research Square.
ABSTRACT Toehold switches are biosensors useful for the detection of endogenous and environmental RNAs. They have been engineered to detect virus RNAs in cell-free gene expression reactions. Their inherent sequence programmability makes engineering a fast predictable process. Despite improvements design, toehold suffer from leaky translation OFF state, which compromises fold change sensitivity biosensor. To address this, we constructed tested signal amplification circuits three triggered by...
Abstract The authors have requested that this preprint be removed from Research Square.