- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Mosquito-borne diseases and control
- Insect-Plant Interactions and Control
- Viral Infections and Vectors
- Plant-Microbe Interactions and Immunity
- vaccines and immunoinformatics approaches
- Plant Pathogenic Bacteria Studies
- Insect symbiosis and bacterial influences
- Irrigation Practices and Water Management
- Insect Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
- Banana Cultivation and Research
- Plant Parasitism and Resistance
- Sugarcane Cultivation and Processing
- Insect Pest Control Strategies
- Insect behavior and control techniques
- Transgenic Plants and Applications
- Legume Nitrogen Fixing Symbiosis
- Parasite Biology and Host Interactions
- Plant Disease Management Techniques
- Parasitic Infections and Diagnostics
- Plant pathogens and resistance mechanisms
- Occupational health in dentistry
- Yeasts and Rust Fungi Studies
Baylor College of Medicine
2025
Universidade de Brasília
2012-2023
Fundação Faculdade de Medicina
2019
Graduate School Experimental Plant Sciences
2016-2017
State Key Laboratory of Virology
2015
Wageningen University & Research
2014-2015
Although the Sw-5 gene cluster has been cloned, and Sw-5b identified as functional copy that confers resistance to Tomato spotted wilt virus (TSWV), its avirulence (Avr) determinant not date. Nicotiana tabacum 'SR1' plants transformed with a of are immune without producing clear visual response on challenge TSWV, whereas it is shown here N. benthamiana gives rapid conspicuous hypersensitive (HR). Using these plants, from all structural non-structural TSWV proteins tested, cell-to-cell...
Tospoviruses (Genus Tospovirus, Family Bunyaviridae) are phytopathogens responsible for significant worldwide crop losses. They have a tripartite negative and ambisense RNA genome segments, termed S (Small), M (Medium) L (Large) RNA. The vector-transmission is mediated by thrips in circulative-propagative manner. For new tospovirus species acceptance, several analyses needed, e.g., the determination of viral protein sequences enlightenment their evolutionary history.Biological (host range...
Only a limited number of dominant resistance genes acting against plant viruses have been cloned, and further functional studies these almost entirely to the Rx Potato virus X (PVX) N Tobacco mosaic (TMV). Recently, cell-to-cell movement protein (NSM ) Tomato spotted wilt (TSWV) has identified as avirulence determinant (Avr) Sw-5b-mediated resistance, gene which belongs class SD-CC-NB-LRR (Solanaceae domain-coiled coil-nucleotide-binding-leucine-rich repeat, SD-CNL) genes. On transient...
The Sw-5 gene cluster encodes protein receptors that are potentially able to recognize microbial products and activate signaling pathways lead plant cell immunity. Although there several homologs in the tomato genome, only one of them, named Sw-5b, has been extensively studied due its functionality against a wide range (thrips-transmitted) orthotospoviruses. Sw-5b is dominant resistance originally from wild Peruvian used breeding programs aiming develop cultivars with these viruses. Here, we...
Abstract Background Soil is a reservoir for many parasites that can affect human and animal health, especially in tropical regions where soil-transmitted helminths protozoa thrive. Understanding how environmental factors influence parasite distribution will provide basis relating climate changes may intensify their impacts, altering habitats increasing transmission risks. We surveyed soil prevalence, burden, diversity several different Peruvian ecologies to catalog current presence baseline...
Plant vegetative propagation strategies for agricultural crops cause the accumulation of viruses, resulting in formation virus complexes or communities. The cultivation garlic is based on and more than 13 species from genera Potyvirus , Allexivirus Carlavirus have been reported. Aiming an unbiased identification viruses a germplasm collection Brazil, total RNA eight cultivars was sequenced by high-throughput sequencing (HTS) technology. Although most found this study were previously...
Mayaro virus (MAYV) is responsible for a mosquito-borne tropical disease with clinical symptoms similar to dengue or chikungunya fevers. In addition the recent territorial expansion of MAYV, this may be an increasing number outbreaks. Currently, no vaccine available. Aedes aegypti promiscuous in its viral transmission and thus interesting model understand MAYV-vector interactions. While life-cycle MAYV known, mechanisms by which arbovirus affects mosquito host cells are not clearly...
Tobamoviruses are often referred to as the most notorious viral pathogens of pepper crops. These viruses not transmitted by invertebrate vectors, but rather physical contact and seeds. In this study, plants displaying mild mottle mosaic symptoms were sampled in four different regions Peru. Upon double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) tests, seven samples cross-reacted weakly with antibodies against virus (PMMoV), suggesting presence tobamoviruses. When...
Zucchini lethal chlorosis virus (ZLCV) causes significant losses in the production of cucurbits Brazil. This belongs to genus Tospovirus (family Bunyaviridae) and seems be exclusively transmitted by Frankliniella zucchini (Thysanoptera). Tospoviruses have a tripartite single-stranded RNA genome classified as S (Small), M (Medium) L (Large) RNAS. Although ZLCV was identified member 1999, its complete had not been sequenced until now. We full-length two isolates named ZLCV-SP ZLCV-DF. The...
Tomato chlorotic spot virus (TCSV) and groundnut ringspot (GRSV) share several genetic biological traits. Both of them belong to the genus Tospovirus (family Peribunyaviridae), which is composed by viruses with tripartite RNA genome that infect plants are transmitted thrips (order Thysanoptera). Previous studies have suggested reassortment events between these two viruses, some speculated they may one their genomic segments. To better understand intimate evolutionary history we sequenced...
The Tomato chlorotic spot virus (TCSV) was first reported in the 1980s, having its occurrence limited to Brazil and Argentina. Due an apparent mild severity past, molecular studies concerning TCSV were neglected. However, has disseminated over USA Caribbean countries. In Dominican Republic been recently on important cultivated crops such as pepper beans. this work, we provide complete genome of a isolate from symptomatic plants Republic, which obtained by high-throughput sequencing....
Chikungunya virus (CHIKV) is a single-stranded positive RNA that belongs to the genus Alphavirus and transmitted humans by infected Aedes aegypti albopictus bites. In humans, CHIKV usually causes painful symptoms during acute chronic stages of infection. Conversely, virus–vector interaction does not disturb mosquito’s fitness, allowing persistent Herein, we studied infection Ae. Aag-2 cells (multiplicity (MOI) 0.1) for 48 h through label-free quantitative proteomic analysis transmission...
Although RNA viruses have high mutation rates, host cells and organisms work as selective environments, maintaining the viability of virus populations by eliminating deleterious genotypes. In serial passages in a single cell line, most these bottlenecks are absent, with no circulation replication different tissues or alternation. this work, Aedes aegypti Aag-2 were accidentally infected Chikungunya (CHIKV) Mayaro (MAYV). After numerous to achieve infection persistency, infectivity was...