A5009064128- Marine Ecology and Invasive Species
- Developmental Biology and Gene Regulation
- Marine Biology and Environmental Chemistry
- Neurobiology and Insect Physiology Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Marine Sponges and Natural Products
- Congenital heart defects research
- Neuroendocrine regulation and behavior
- Marine Invertebrate Physiology and Ecology
- Neuropeptides and Animal Physiology
- Reproductive biology and impacts on aquatic species
- Invertebrate Immune Response Mechanisms
- Zebrafish Biomedical Research Applications
- Identification and Quantification in Food
- RNA Research and Splicing
- Marine Biology and Ecology Research
- Echinoderm biology and ecology
- Sperm and Testicular Function
- Physiological and biochemical adaptations
- Cephalopods and Marine Biology
- Ichthyology and Marine Biology
- Hedgehog Signaling Pathway Studies
- Cardiomyopathy and Myosin Studies
- Cellular Mechanics and Interactions
- Wnt/β-catenin signaling in development and cancer
Chiba University
2014-2024
National College Of Nursing
2019
Kochi Medical School Hospital
2010
Kyoto University
1996-2002
Tokushima University
1996
The University of Tokyo
1960-1968
Japanese Red Cross Hiroshima College of Nursing
1967
Abstract Background The draft genome sequence of the ascidian Ciona intestinalis , along with associated gene models, has been a valuable research resource. However, recently accumulated expressed tag (EST)/cDNA data have revealed numerous inconsistencies models due in part to intrinsic limitations prediction programs and fragmented nature assembly. Results We prepared less-fragmented assembly on basis scaffold-joining guided by paired-end EST bacterial artificial chromosome (BAC) sequences,...
The Pax6 gene plays a developmental role in various metazoans as the master regulatory for eye patterning. is also spatially regulated particular regions of neural tube. Because amphioxus has no neuromeres, an understanding expression agnathans crucial insight into origin neuromerism vertebrates. We have isolated single cognate cDNA gene, LjPax6, from Lampetra japonica library and observed pattern its using situ hybridization. Phylogenetic analysis revealed that LjPax6 occurs sister group...
ABSTRACT The epithelium of the pharynx contributes to formation gills in hemichordates, urochordates, cephalochordates and primitive vertebrates, is therefore a key structure for understanding developmental mechanisms underlying establishment chordate body plans. Pax1- Pax9-related genes encode transcription factors which are expressed pharyngeal region as well vertebrate pouch that forms thymus parathyroid glands. To explore molecular basis occurrence modifications during evolution, we...
The endostyle is a pharyngeal organ for the internal filter feeding of urochordates, cephalochordates, and larval lamprey. This also considered to be homologous follicular thyroid gland higher vertebrates. Thyroglobulin (Tg) peroxidase (TPO) are specifically expressed in vertebrates, they play an important role iodine metabolism synthesis hormones. Previous histochemical observations showed that iodine-concentrating activities were detected zones 7, 8, 9 ascidian endostyle, suggesting these...
Key transmembrane proteins in the innate immune system, Toll-like receptors (TLRs), have been suggested to occur genome of non-mammalian organisms including invertebrates. However, authentic invertebrate TLRs neither structurally nor functionally investigated. In this paper, we originally present structures, localization, ligand recognition, activities, and inflammatory cytokine production all ascidian Ciona intestinalis, designated as Ci-TLR1 Ci-TLR2. The amino acid sequence Ci-TLR2 were...
The phylogenetic position of ascidians as the chordate invertebrates closest to vertebrates suggests that they might possess homologs and/or prototypes vertebrate peptide hormones and neuropeptides well ascidian-specific peptides. However, only a small number peptides have so far been identified in ascidians. In present study, we various ascidian, Ciona intestinalis. Mass spectrometry-based peptidomic analysis detected 33 peptides, including 26 novel from C. ascidian are largely classified...
The calcitonin (CT)/CT gene‐related peptides (CGRPs) constitute a large peptide family in vertebrates. However, no CT/CGRP superfamily members have so far been identified invertebrates, and the evolutionary process leading to diverse vertebrate remains unclear. In this study, we an authentic invertebrate CT, Ci‐CT, ascidian Ciona intestinalis , which is phylogenetically closest chordate amino acid sequence of Ci‐CT was shown display high similarity those CTs share CT consensus motifs,...
The ascidian egg is a well-known mosaic egg. In order to investigate the molecular nature of maternal genetic information stored in egg, we have prepared cDNAs from mRNAs fertilized eggs ascidian, Halocynthia roretzi. embryo were sequenced, and localization individual mRNA was examined staged embryos by whole-mount situ hybridization. data obtained database MAGEST (http://www.genome.ad.jp/magest) further analyzed. A total 4240 cDNA clones found represent 2221 gene transcripts, including at...
Abstract The endostyle of invertebrate chordates is a pharyngeal organ that thought to be homologous with the follicular thyroid vertebrates. Although thyroid‐like features such as iodine‐concentrating and peroxidase activities are located in dorsolateral part both ascidian amphioxus endostyles, structural organization numbers functional units different. To estimate phylogenetic relationships each zone special reference evolution thyroid, we have investigated, amphioxus, expression patterns...
Cholecystokinin (CCK) and gastrin are vertebrate brain–gut peptides featured by a sulfated tyrosine residue C-terminally amidated tetrapeptide consensus sequence. Cionin, identified in the ascidian, Ciona intestinalis , closest species to vertebrates, harbors two tyrosines CCK/gastrin While putative cionin receptor, cior was cloned, ligand–receptor relationship between CioR remains unidentified. Here, we identify receptors, CioR1 CioR2, which aforementioned receptor its novel paralog...
Recent progress in multiple and automated-sequencing technology allows large-scale random cDNA sequencing, the so-called EST project, various fields. In addition to collection, project requires analysis of spatiotemporal patterns gene expression a large number clones by whole-mount situ hybridization (WISH). To facilitate WISH procedures, we developed protocol for rapid uniform synthesis probes multi-well based processing. A DIG-labeled RNA probe was synthesized from PCR-amplified template...
The Ciona intestinalis protein database (CIPRO) is an integrated for the tunicate species C. . unique in two respects: first, because of its phylogenetic position, suitable model understanding vertebrate evolution; and second, includes original large-scale transcriptomic proteomic data. has also been a favorite developmental biologists. Therefore, large amounts data exist on development morphology, along with recent genome sequence gene expression CIPRO aimed at collecting those published as...