A5101925652- Insect-Plant Interactions and Control
- Plant and animal studies
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Insect and Pesticide Research
- Plant tissue culture and regeneration
- Enzyme-mediated dye degradation
- Hymenoptera taxonomy and phylogeny
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Insect Pheromone Research and Control
- Plant nutrient uptake and metabolism
- Fungal Biology and Applications
- Biochemical and biochemical processes
- Insect and Arachnid Ecology and Behavior
- Phytoplasmas and Hemiptera pathogens
- Insect behavior and control techniques
- Polysaccharides and Plant Cell Walls
- Lignin and Wood Chemistry
- Horticultural and Viticultural Research
- Insect symbiosis and bacterial influences
- RNA and protein synthesis mechanisms
- Lepidoptera: Biology and Taxonomy
- Fermentation and Sensory Analysis
- Forest Insect Ecology and Management
Institute of Fruit Tree and Tea Science
2004-2024
Ehime University
2009-2022
Meiji University
2022
National Agriculture and Food Research Organization
2009-2021
Ishihara Sangyo Kaisha (Japan)
2021
Central Research Institute
2021
Institute for Sustainable Plant Protection
2021
Institute of Vegetable and Floriculture Science
2005-2016
Kyushu Institute of Technology
2015
Tohoku University
1993-1997
As a defense response to attacks by herbivores such as the smaller tea tortrix (Adoxophyes honmaiYasuda), (Camellia sinensis) leaves emit numerous volatiles (Z)-3-hexen-1-ol, linalool, α-farnesene, benzyl nitrile, indole, nerolidol, and ocimenes in higher concentration. Attack of Kanzawa spider mites (Tetranychus kanzawai Kishida), another major pest insect crops, induced emission α-farnesene from leaves. The exogenous application jasmonic acid volatile blend that was similar, although not...
Many land plants evolved tall and sturdy growth habits due to specialized cells with thick lignified cell walls: tracheary elements that function in water transport fibers structural support. The objective of this study was define how when diverse populations contribute lignin precursors, monolignols, secondary walls during lignification the Arabidopsis (Arabidopsis thaliana) inflorescence stem. Previous work demonstrated that, biosynthesis is suppressed fiber element thickened walls, become...
Tracheary element (TE) differentiation is a typical example of programmed cell death (PCD) in higher plants, and maturation TEs completed by degradation all contents. However, lignification progresses even after PCD. We investigated how whence monolignols are supplied to which have undergone PCD during isolated Zinnia mesophyll cells into TEs. Higher densities culture induced greater Whereas the continuous exchanging medium suppressed TEs, further addition coniferyl alcohol reduced...
We have investigated the mechanism of lignification during tracheary element (TE) differentiation using a Zinnia elegans xylogenic culture. In process, we isolated ZPO-C, peroxidase gene Z. that is expressed specifically in differentiating TEs. ZPO-C suggested to be involved TEs vivo and vitro. Furthermore, Arabidopsis thaliana (AtPrx66), which homologous was identified. The expression profile functions planta remain investigated. this study, performed promoter::beta-glucuronidase (GUS)...
Heme is involved in various biological processes as a cofactor of hemoproteins located organelles. In plant cells, heme synthesized by two isoforms plastid-localized ferrochelatase, FC1 and FC2. this study, characterizing Arabidopsis T-DNA insertional mutants, we showed that the allocation differentially regulated ferrochelatase cells. Analyses weak (fc1-1) null (fc1-2) mutants suggest FC1-producing required for initial growth seedling development. contrast, (fc2-1) (fc2-2) FC2 pale green...
During differentiation of isolated Zinnia mesophyll cells into tracheary elements (TEs), lignification on TEs progresses by supply monolignols not only from themselves but also surrounding xylem parenchyma-like through the culture medium. However, how lignin polymerizes secreted has been resolved. In this study, we analyzed phenol compounds in medium with reversed-phase HPLC, gas chromatography–mass spectrometry and nuclear magnetic resonance spectrometry, found 12 phenolic including...
In an attempt to elucidate the regulatory mechanism of vessel lignification, we isolated ZPO-C, a novel peroxidase gene Zinnia elegans that is expressed specifically in differentiating tracheary elements (TEs). The ZPO-C transcript was shown accumulate transiently at time secondary wall thickening TEs xylogenic culture cells. situ hybridization indicated specific accumulation immature vessels seedlings. Immunohistochemical analysis using anti-ZPO-C antibody showed protein abundant TEs,...
The citrus spiny whitefly Aleurocanthus spiniferus (Quaintance) is a pest of plants that native to South-East Asia. Although serious outbreaks the tea-infesting in China, Taiwan and Japan have been attributed this species over last 20 years, recent research has shown different host preferences between two whiteflies. Hence, pests tentatively differentiated as citrus-infesting populations. We further compared morphological, acoustic genomic features populations Japan. Morphological...
To study the regulatory mechanisms underlying lignin biosynthesis, we isolated and characterized lignescens (lig), a previously undescribed temperature-sensitive mutant of Arabidopsis thaliana that exhibits ectopic deposition growth defects under high-temperature conditions. The lig mutation was identified as single base transition in GNA1 encoding glucosamine-6-phosphate N-acetyltransferase (GNA), critical enzyme UDP-N-acetylglucosamine (UDP-GlcNAc) biosynthesis. harbors glycine-to-serine...
Uridine diphosphate‐ N ‐acetylglucosamine (UDP‐GlcNAc) donates GlcNAc for various glycans and glycoconjugates. We previously found that supplementation increases the UDP‐GlcNAc content in Arabidopsis ; however, metabolic pathway was undefined. Here, we show homolog of human kinase (GNK) is conserved land plants. Enzymatic assays homologous protein (AtGNK) revealed activity highly specific GlcNAc. also demonstrate role AtGNK plants by using its knockout mutant, which presents lower contents...
We have found that the spectral sensitivity of compound eye in summer fruit tortrix moth (Adoxophyes orana) differs laboratory strains originating from different regions Japan. investigated mechanisms underlying this anomalous sensitivity. applied electrophysiology, light and electron microscopy, opsin gene cloning, mathematical modeling, behavioral analysis. The ERG-determined dark-adapted individuals all peaks around 520 nm. When light-adapted, Nagano strain narrows its peak shifts to 580...
Although mechanisms that activate organogenesis in plants are well established, much less is known about the subsequent fine-tuning of cell proliferation, which crucial for creating properly structured and sized organs. Here we show, through analysis temperature-dependent fasciation (TDF) mutants Arabidopsis, root redifferentiation defective 1 ( rrd1 ), rrd2 , initiation 4 rid4 mitochondrial RNA processing required limiting division during early lateral (LR) organogenesis. These formed...
The invasive orange spiny whitefly (OSW) Aleurocanthus spiniferus has extended its distribution to non-native areas since the early 20th century. In a similar manner, tea (TSW) A. camelliae been expanding over East Asia in recent decades. this study, genetic diversity of OSW and TSW their important parasitoid wasp Encarsia smithi was investigated China Japan enable more efficient biological control policies. We detected two phylogenetic groups (haplogroups A1 A2) three (haplotypes B1 B2,...
Abstract Changes in the enzymatic activity of cinnamyl alcohol dehydrogenase (CAD) and expression a gene for CAD during tracheary element (TE) differentiation were investigated cultures single cells isolated from mesophyll zinnia (Zinnia elegans). In which TE was induced (TE-inductive cultures), increased h 36 after start culture (12 before thickening secondary cell wall) peaked at 72, when lignin actively being deposited. control not induced, remained very low level 5 d. Some isoforms...
N-acetylglucosamine (GlcNAc) is the fundamental amino sugar moiety that essential for protein glycosylation. UDP-GlcNAc, an active form of GlcNAc, synthesized through hexosamine biosynthetic pathway (HBP). Arabidopsis N-acetylglucosamine-1-P uridylyltransferases (GlcNAc1pUTs), encoded by GlcNA.UTs, catalyze last step in HBP pathway, but their biochemical and molecular functions are less clear. In this study, GlcNA.UT1 expression was knocked down double-stranded RNA interference (dsRNAi)...
Abstract The tea green leafhopper, Empoasca onukii Matsuda (Hemiptera: Cicadellidae, Typhlocybinae), is a serious pest of plants in East Asia. In the laboratory, genotypes CA278, Cd19, and Cd289 [ Camellia sinensis (L.) O. Kuntze (Theaceae)] sustain fewer feeding damage symptoms known as hopperburn than susceptible cultivar ‘Yabukita,’ E. excretes less honeydew on them Yabukita. Here, we evaluated whether these have practical sufficient resistance under field conditions. densities eggs,...