A5070230890- Oral microbiology and periodontitis research
- Genomics and Phylogenetic Studies
- Oral Health Pathology and Treatment
- HIV/AIDS oral health manifestations
- Bacterial Identification and Susceptibility Testing
- Infective Endocarditis Diagnosis and Management
- Streptococcal Infections and Treatments
- Endodontics and Root Canal Treatments
- Dental Health and Care Utilization
- Gut microbiota and health
- Salivary Gland Disorders and Functions
- Probiotics and Fermented Foods
- Dental Research and COVID-19
- Infant Nutrition and Health
- Otolaryngology and Infectious Diseases
- Infections and bacterial resistance
- Dental Radiography and Imaging
- Microbial infections and disease research
- Rabies epidemiology and control
- Bacteriophages and microbial interactions
- Plant Pathogens and Fungal Diseases
- Infection Control and Ventilation
- Dental Anxiety and Anesthesia Techniques
- Preterm Birth and Chorioamnionitis
- Nosocomial Infections in ICU
King's College London
2011-2024
Queen Mary University of London
2014-2023
Guy's Hospital
1996-2019
St Thomas' Hospital
2006-2012
Royal London Hospital
2012
University College London
2010-2012
University of London
2010
Guy's and St Thomas' NHS Foundation Trust
2002
Cardiff University
1990-2002
University of Wales
1987-1998
The human oral cavity contains a number of different habitats, including the teeth, gingival sulcus, tongue, cheeks, hard and soft palates, tonsils, which are colonized by bacteria. microbiome is comprised over 600 prevalent taxa at species level, with distinct subsets predominating habitats. has been extensively characterized cultivation culture-independent molecular methods such as 16S rRNA cloning. Unfortunately, vast majority unnamed referenced clone numbers or GenBank accession numbers,...
We report the design and evaluation of PCR primers 63f 1387r for amplification 16S rRNA genes from bacteria. Their specificity efficacy were tested systematically with a bacterial species environmental samples. They found to be more useful gene in ecological systematic studies than amplimers that are currently generally used.
For millions of years, our resident microbes have coevolved and coexisted with us in a mostly harmonious symbiotic relationship. We are not distinct entities from microbiome, but together we form 'superorganism' or holobiont, the microbiome playing significant role physiology health. The mouth houses second most diverse microbial community body, harbouring over 700 species bacteria that colonise hard surfaces teeth soft tissues oral mucosa. Through recent advances technology, started to...
Molecular techniques have revealed many novel, presumed unculturable, taxa in oral infections. The aim of this study was to characterize the bacterial community middle and advancing front carious dental lesions by cultural molecular analyses. Samples were collected with a hand excavator from five teeth involving dentine. cultured on blood agar Rogosa incubated air plus 5% CO(2) fastidious anaerobe anaerobically. DNA also extracted directly samples 16S rRNA genes amplified PCR universal...
Background: The beneficial cardiovascular effects of vegetables may be underpinned by their high inorganic nitrate content. Objective: We sought to examine the a 6-wk once-daily intake dietary (nitrate-rich beetroot juice) compared with placebo (nitrate-depleted on vascular and platelet function in untreated hypercholesterolemics. Design: A total 69 subjects were recruited this randomized, double-blind, placebo-controlled parallel study. primary endpoint was change determined use ultrasound...
Current knowledge of the microbial composition dental plaque in early gingivitis is based largely on microscopy and cultural methods, which do not provide a comprehensive description oral communities. This study used 454-pyrosequencing V1–V3 region 16S rRNA genes (approximately 500 bp), bacterial culture, to characterize during transition from periodontal health gingivitis. A total 20 healthy volunteers abstained hygiene for two weeks, allowing accumulate develop. Plaque samples were...
Abstract Controversy exists concerning the mode of action chlorhexidine in plaque inhibition. This study attempted to determine whether an oral reservoir was necessary for Plaque growth on enamel under influence topically applied or rinsed closely monitored by clinical scoring, bacterial culturing and scanning electron microscopy. Thus, 3 subjects wore removable acrylic appliances containing inserts. In first regimen, inserts one side were exposed 0.2% other, water 1 min twice a day 14 days....
The first bacterial culture media were broths made either by infusion or enzymatic digestion of meat from various sources. Originally developed Spallanzani in the 18th century and then refined Pasteur 19th century, these allowed recovery bacteria human disease sites1,2. However, it was quickly realized that such would be likely to contain mixtures micro-organisms, Robert Koch saw need for development solid allow physical separation colonies. He tried aseptically divided potatoes. Material...
Five strains of anaerobic, Gram-negative bacilli isolated from the human oral cavity were subjected to a comprehensive range phenotypic and genotypic tests found comprise homogeneous group. Phylogenetic analysis full-length 16S rRNA gene sequences showed that these represented novel group within family Prevotellaceae , most closely related species was Prevotella tannerae . P. taxon are deeply branched genus with sequence identities type strain melaninogenica 82.2 85.6 %, respectively. The...
In order to characterize the bacterial microbiota present within oral cancerous lesions, tumorous and non-tumorous mucosal tissue specimens (approx. 1 cm 3 ) were harvested from ten squamous cell carcinoma (OSCC) patients at time of surgery. Any microbial contamination on surface was eliminated by immersion in Betadine washing with PBS. Bacteria visualized sections OSCC performing fluorescent situ hybridization universal oligonucleotide probe, EUB338. DNA extracted each aseptically macerated...
Introduction: Molecular ecological analysis based on 16S rRNA gene sequence is well established for the characterisation of complex bacterial communities. However, ‘universal’ PCR primers can introduce biases into species composition clone libraries because mismatches between primer and target organism sequences. In this study, three universal sets were compared microflora in subgingival plaque. Methods: Three plaque samples collected from two subjects with localised severe chronic...
Over a third of oral bacteria are as-yet-uncultivated in-vitro. Siderophores have been previously shown to enable in-vitro growth uncultivated bacteria. The objective this study was cultivate novel in siderophore-supplemented culture media. Various compounds with siderophore activity, including pyoverdines-Fe-complex, desferricoprogen and salicylic acid, were found stimulate the difficult-to-culture strains Prevotella sp. HOT-376 Fretibacterium fastidiosum. Furthermore, pyrosequencing...
Objective To determine the effects on vaginal microbiota of an oral probiotic preparation administered from early pregnancy. Design Randomised, double blind, placebo‐controlled trial. Setting Four maternity units in UK . Population Women aged 16 years or older recruited at 9–14 weeks' gestation. Methods Participants were randomly allocated to receive capsules containing Lactobacillus rhamnosus GR ‐1 and reuteri RC ‐14 each 2.5 × 10 9 colony‐forming ( CFU s) placebo once daily recruitment...
The microflora associated with three dentoalveolar abscesses was determined by cultural and molecular methods. 16S rRNA genes were randomly amplified means of conserved eubacterial primers cloned. Restriction fragment length polymorphism analysis the clones encoding from cultured bacteria used to detect putative unculturable bacteria. Clones representative five predominant groups uncultured organisms sequenced. Two identified as Porphyromonas gingivalis Prevotella oris, one found be closely...
The diversity of organisms present in the subgingival pockets patients with periodontitis and acute necrotizing ulcerative gingivitis (ANUG) were examined previously. 16S rRNA genes plaque bacteria amplified using PCR a universal forward primer spirochaete-selective reverse primer. DNA was cloned into Escherichia coli. In one subject ANUG, 70 clones sequenced. Seventy-five per cent spirochaetal, as expected. Twelve remaining fell two clusters that represent novel phylotypes family...
16S rRNA gene sequences were determined for Eubacterium exiguum and Peptostreptococcus heliotrinreducens. These species found to be closely related and, together with lentum, constitute a branch of the Coriobacteriaceae. Two new genera are proposed on basis phenotypic characteristics sequence comparisons: Slackia include bile-sensitive P. heliotrinreducens, Eggerthella bile-resistant lentum. It is that heliotrinreducens transferred genus gen. nov. as exigua nov., comb. (type strain ATCC...