A5085646338- Force Microscopy Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Microbial Inactivation Methods
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- thermodynamics and calorimetric analyses
- Mechanical and Optical Resonators
- Cancer therapeutics and mechanisms
- Supramolecular Self-Assembly in Materials
- RNA Interference and Gene Delivery
- Molecular Junctions and Nanostructures
- Analytical Chemistry and Sensors
- Photoreceptor and optogenetics research
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- DNA and Nucleic Acid Chemistry
- Electrowetting and Microfluidic Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Transgenic Plants and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Molecular Sensors and Ion Detection
- Advanced Fluorescence Microscopy Techniques
- Amino Acid Enzymes and Metabolism
Clarus Therapeutics (United States)
2025
Centre National de la Recherche Scientifique
2013-2024
École Normale Supérieure - PSL
2004-2024
Inserm
1982-2024
Institut de Biologie de l'École Normale Supérieure
2019-2024
Université Paris Sciences et Lettres
2019-2024
Laboratoire Photonique, Numérique et Nanosciences
2011-2021
Laboratoire de Géologie de l’École Normale Supérieure
2021
Amway (United States)
2015
Physique des Cellules et Cancers
2004-2010
The nematode Caenorhabditis elegans has been central to the understanding of metazoan biology. However, C. is but one species among millions and significance this important model organism will only be fully revealed if it placed in a rich evolutionary context. Global sampling efforts have led discovery over 50 putative from genus Caenorhabditis, many which await formal description. Here, we present descriptions for 10 new species. We also draft genome sequences nine these species, along with...
Anti-D66 is a monoclonal antibody able to inhibit E-rosette formation of T cells both at 4 degrees C and 37 degree but that does not cell rosette with neuraminidase or 2-amino-ethylisothiouronium bromide (AET)-pretreated E. As demonstrated by capping experiments, it defines an epitope, D66, directly involved in formation. D66 distinct from the epitope defined 9.6 because 9.6, previously "pan-T" antibody, inhibits E(AET) no cross-blocking occurred between antibodies fixation. However, are...
We describe a method of controlled evaporation on textured substrate for self-assembling and shaping gold-nanorod-based materials. Tridimensional wall features are formed over areas as large several square millimeters. Furthermore, analyses by small-angle X-ray scattering scanning electron microscopy techniques demonstrate that colloids locally ordered smectic B phase. Such crystallization is in fact possible because we could finely adjust the nanoparticle charge, knowledge additionally...
We present an experimental study of the microfluidic electrophoresis long DNA in self-assembling matrixes magnetic bead columns. Results are presented for rapid separation lambda-phage, 2lambda-DNA, and bacteriophage T4 DNA, where resolutions greater than 2 between lambda achieved times as short 150 s. The use a computer-piloted flow control system injection results high reproducibility separations. compare experimentally measured mobility dispersion with exactly solvable lattice Monte Carlo...
Molecular beacons (MBs) are fluorescent nucleic acid probes with a hairpin-shaped structure in which the 5' and 3' ends self-complementary. Due to change their emissive properties upon recognition complementary sequences, MBs allow diagnosis of single-stranded DNA or RNA high mismatch discrimination, vitro vivo. Whereas stems MB hairpins usually rely on formation Watson−Crick duplex, we demonstrate this report that preceding can be replaced by G-quadruplex motif (G4). Intramolecular...
To facilitate thermal imaging, particularly in microdevices, one has to favor molecular thermometers which the response is independent of probe concentration and observation setup imperfections. Hence, this paper introduces two temperature fluorescent probes for ratiometric dual-emission-wavelength measurements aqueous solutions. They are based on a nonathermal chemical reaction, either conformational transition or protonation, that induces modification their emission spectra as changes....
Equimolar mixtures of large unilamellar vesicles (LUVs) obtained from egg lecithin and lipids containing complementary hydrogen bonding head groups (barbituric acid (BAR) 2,4,6-triaminopyrimidine (TAP)) were shown to aggregate fuse. These events have been studied in detail using electron microscopy dynamic light scattering, by fluorimetry membrane or water-soluble fluorescence probes. It was that aggregation followed two competitive processes: a) lipid mixing leading redispersion the...
Equimolar mixtures of large unilamellar vesicles (LUVs) obtained from egg lecithin and lipids containing complementary hydrogen bonding head groups (barbituric acid (BAR) 2,4,6triaminopyrimidine (TAP)) were shown to aggregate fuse. These events have been studied in detail using electron microscopy dynamic light scattering, by fluorimetry membrane or water-soluble fluorescence probes. It was that aggregation followed two competitive processes : a) lipid mixing leading redispersion the ; b)...
This report examines the organization properties of new fluorescent DNA-lipids, either alone in water or interaction with 1-octyl-beta-d-glucopyranoside micelles egg phosphatidylcholine vesicles. We first describe design and syntheses conjugates. Then, we use UV-Vis absorption, steady-state fluorescence emission, electron microscopy, correlation spectroscopy after two-photon excitation to show that these DNA-lipids form spherical aqueous solution incorporate much better than also investigate...
Abstract Corrosion of flexible risers armor wires resulting in wire breakage with subsequent rupture is a credible risk for the offshore energy industry. LLOG has been operating Who Dat since 2011. After 12 years service, baseline annulus condition had not conducted due to inspection and monitoring constraints from riser configuration. This paper outlines approach select validate suitable technologies reliably identify tensile anomalies. The can vary significantly each directly affects...
We present a new technique to measure the mechanical properties of small biomolecules. This uses long range repulsive colloidal forces together with magnetic attraction as force probing tool. The biomolecules are grafted between superparamagnetic particles, which regularly spaced within chains maintained by an external field. Varying field results in compression or extension molecules particles. In order demonstrate this we use, size controlled model molecule, short double stranded DNA (151...
To fabricate microlaboratories, commercially available silica glasses represent a good alternative to the expensive quartz or fused substrates. Therefore, authors have here investigated behavior of four them—Vycor, Pyrex, D263, and AF45—in SF6 SF6/Ar inductively coupled plasmas. Using Vycor, material close pure SiO2, as reference, they demonstrated that etch rate negatively correlates with global content in metallic oxides. However, no such clear trend was found for surface roughness...
Integrins are adhesion receptors that mediate cell and play an important function in many biological processes such as morphogenesis tissue remodeling. These membrane proteins specifically interact with a short tripeptide sequence, RGD (Arg-Gly-Asp), present numerous extracellular macromolecules. Model systems have been developed order to understand how is induced by this specific peptide ligand/integrin recognition system. We previously shown giant vesicles selectively adhere endothelial...
Biological samples exhibit huge molecular diversity over large concentration ranges. Titrating a given compound in such mixtures is often difficult, and innovative strategies emphasizing selectivity are thus demanded. To overcome limitations inherent to thermodynamics, we here present generic technique where discrimination relies on the dynamics of interaction between target interest probe introduced excess. Considering an ensemble two-state exchanging reactants submitted temperature...
The description of interactions between biochemical species and the elucidation corresponding chemical mechanisms encounter an increasing interest both for comprehension biological pathways at molecular scale rationalization drug design. Relying on powerful experimental tools such as thermal microfluidics fluorescence detection, we propose a methodology to determine mechanism reaction without fitting parameters. A consistent with accessible knowledge is assumed, assumption checked through...
The ability to follow and modify cell behaviour with accurate spatiotemporal resolution is a prerequisite study morphogenesis in developing organisms. Electroporation, the delivery of exogenous molecules into targeted populations through electric permeation plasma membrane, has been used this aim different model systems. However, current localised electroporation strategies suffer from insufficient reproducibility mediocre survival when applied small delicate organisms such as early...