A5038157206- Antimicrobial Resistance in Staphylococcus
- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
- Helicobacter pylori-related gastroenterology studies
- Microbial infections and disease research
- Streptococcal Infections and Treatments
- Restless Legs Syndrome Research
- Genomics and Phylogenetic Studies
- Tuberculosis Research and Epidemiology
- Urinary Tract Infections Management
- Microscopic Colitis
- Viral gastroenteritis research and epidemiology
- Neonatal and Maternal Infections
- Data-Driven Disease Surveillance
- Antibiotics Pharmacokinetics and Efficacy
- Salmonella and Campylobacter epidemiology
- Enterobacteriaceae and Cronobacter Research
- Cell Image Analysis Techniques
- Bacterial biofilms and quorum sensing
- Child Nutrition and Feeding Issues
Institute of Environmental Science and Research
2021-2024
Micropore Technologies (United Kingdom)
2002-2004
Abstract In this study, the distribution of macrolide resistance mechanisms was determined for isolates Streptococcus pneumoniae and pyogenes obtained from PROTEKT 1999–2000 study (a global, longitudinal antibacterial susceptibility bacterial pathogens associated with community-acquired lower respiratory tract infections). The global mechanism results 1043 macrolide-resistant S. collected 25 countries were as follows: 35.3% mef (A) , 56.2% erm (B) 6.8% both 0.2% subclass (TR) 1.5% negative...
Sixteen (1.5%) of the 1,043 clinical macrolide-resistant Streptococcus pneumoniae isolates collected and analyzed in 1999-2000 PROTEKT (Prospective Resistant Organism Tracking Epidemiology for Ketolide Telithromycin) study have resistance mechanisms other than rRNA methylation or efflux. We determined macrolide all 16 by sequencing L4 L22 riboprotein genes, plus relevant segments four genes 23S rRNA, expression mutant rRNAs was primer extension. Isolates from Canada (n = 4), Japan 3),...
ABSTRACT Of a total of 1,043 macrolide-resistant Streptococcus pneumoniae isolates collected from 24 countries as part PROTEKT 1999-2000, 71 tested positive for both the mef (A) and erm (B) genes. 69 subjected to further molecular investigations, all were resistant tetracycline, 63 (91.3%) penicillin, 57 (82.6%) trimethoprim-sulfamethoxazole. One isolate was also fluoroquinolone resistant, another quinupristin-dalfopristin. The ketolide telithromycin retained activity against isolates. these...
ABSTRACT To date, 86 of 7,746 macrolide-resistant Streptococcus pneumoniae isolates from 1999 to 2002 PROTEKT (Prospective Resistant Organism Tracking and Epidemiology for the Ketolide Telithromycin) surveillance studies were negative methylase efflux mechanisms. Mutations in 23S rRNA or genes encoding riboprotein L4 L22 found 77 isolates. Six resistant quinupristin-dalfopristin two linezolid, while telithromycin demonstrated good activities against all
To determine the global distribution of TEM-1 and ROB-1 beta-lactamases in Haemophilus influenzae isolated from patients with community-acquired respiratory tract infection during first 4 years PROTEKT study (1999-2003). investigate activities commonly used antibiotics against these isolates.For 14 870 H. influenzae, MIC testing was performed using NCCLS broth microdilution methodology. For 2225 beta-lactamase-positive (BLP) genes were detected a Taqman PCR method.beta-Lactamase positivity...
Outbreaks of methicillin-resistant Staphylococcus aureus (MRSA) are well described in the neonatal intensive care unit (NICU) setting. Genomics has revolutionized investigation such outbreaks; however, to date, this largely been completed retrospectively and typically relied on short-read platforms. In 2022, our laboratory established a prospective genomic surveillance system using Oxford Nanopore Technologies sequencing for rapid outbreak detection. Herein, system, we describe detection...
Antimicrobial resistance (AMR) presents a significant threat to human health worldwide. One important source of antimicrobial-resistant infections in humans is exposure animals or animal products. In phased survey, we investigated AMR 300 Escherichia coli isolates and enterococci (Enterococcus faecalis E. faecium) each from the carcasses poultry, pigs, very young calves, dairy cattle (food animals); all Salmonella cattle; Campylobacter (Campylobacter jejuni C. coli) poultry. The highest...
In 2011 a novel methicillin resistance gene, mecC, was described in human and bovine Staphylococcus aureus isolates. mecC-positive S. is most commonly associated with livestock wildlife populations across Europe particularly prevalent hedgehogs, but only occasionally causes infections. this study we characterize investigate the origin of two isolates containing mecC genes from New Zealand. The were identified patients severe invasion infection as part bacteraemia study. Whole genome...
Many hospital laboratories have technical capacity to perform whole-genome sequencing but lack bioinformatic expertise analyse sequence data. Sending isolates reference creates delays that can be highly detrimental outbreak responses. The Wellington Regional Hospital laboratory, which lacks on-site bioinformaticians, implemented real-time nanopore-based genomic surveillance has detected several outbreaks at an early stage. This required off-site analysis, often taking weeks. Solu Genomics, a...
In 2011, a novel methicillin resistance gene, mecC , was described in human and bovine Staphylococcus aureus isolates. mecC- positive S. is most commonly associated with livestock wildlife populations across Europe particularly prevalent hedgehogs, but only occasionally causes infections. this study, we characterize investigate the origin of two isolates containing genes from New Zealand. The were identified patients severe invasion infections as part an bacteraemia study. Whole-genome...
Fluoroquinolone resistance in Streptococcus pneumoniae isolated from Hong Kong as part of Prospective Resistant Organism Tracking and Epidemiology for the Ketolide Telithromycin 1999/2000 was found to be due spread Spain(23F)-1 clone (mainly a Spain(23F)-1-14 variant). All isolates were multidrug resistant but susceptible quinupristin-dalfopristin, linezolid, telithromycin. The also occurred among antimicrobial-susceptible isolates, which suggests that primary source this may have been Asia...
Abstract Objectives Our laboratory began prospective genomic surveillance for healthcare-associated organisms in 2022 using Oxford Nanopore Technologies (ONT) sequencing as a standalone platform. This has permitted the early detection of outbreaks but been insufficient single-nucleotide variant (SNV)-level analysis due to lower read accuracy than Illumina sequencing. study aimed determine whether Haplotype-aware ERRor cOrrection (HERRO) ONT data could permit high-resolution comparison...
Clostridioides difficile infection (CDI) is a form of antibiotic-associated infectious diarrhoea resulting in significant morbidity and mortality. Community-acquired disease low-risk individuals increasingly recognised. There are limited New Zealand data published.To determine the incidence location onset CDI cases Manawatu region, further describe demographics, risk factors prevalent C. ribotypes population.We performed an case-control study region between September 2018 2019. Cases were...