A5000655745- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Coastal wetland ecosystem dynamics
- Mycorrhizal Fungi and Plant Interactions
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Wastewater Treatment and Nitrogen Removal
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
- Evolution and Genetic Dynamics
- Botanical Research and Applications
- Pharmaceutical and Antibiotic Environmental Impacts
- Soil Carbon and Nitrogen Dynamics
- Plant Pathogens and Fungal Diseases
- Genomics and Phylogenetic Studies
- Nematode management and characterization studies
Estación Experimental del Zaidín
2012-2024
Consejo Superior de Investigaciones Científicas
2011-2024
Summary The soil microbial community is highly complex and contains a high density of antibiotic‐producing bacteria, making it likely source diverse antibiotic resistance determinants. We used functional metagenomics to search for genes in libraries generated from three different samples, containing 3.6 Gb DNA total. identified 11 new genes: 3 conferring ampicillin, 2 gentamicin, chloramphenicol 4 trimethoprim. One the clones was trimethoprim gene encoding 26.8 kDa protein closely resembling...
Current research on the influence of environmental and physicochemical factors in shaping soil bacterial structure has seldom been approached from a pedological perspective. We studied communities eight soils selected along pedogenic gradient at local scale Mediterranean calcareous mountain (Sierra de María, SE Spain). The results showed that relative abundance Acidobacteria, Canditate division WPS-1, Armatimonadetes decreased whereas Actinobacteria, Bacteroidetes, Proteobacteria increased...
The legume symbiont Sinorhizobium meliloti expresses a plethora of small noncoding RNAs (sRNAs) whose function is mostly unknown. Here, we have functionally characterized two tandemly encoded S. Rm1021 sRNAs that are similar in sequence and structure. Homologous (designated AbcR1 AbcR2) been shown to regulate several ABC transporters the related α-proteobacteria Agrobacterium tumefaciens Brucella abortus. In Rm1021, AbcR2 exhibit divergent unlinked regulation stabilized by RNA chaperone Hfq....
Summary M yxococcus xanthus is a social bacterium that preys on prokaryotic and eukaryotic microorganisms. Co‐culture of . with reference laboratory strains field isolates the legume symbiont S inorhizobium meliloti revealed two different predatory patterns resemble frontal wolf‐pack attacks. Use mutants impaired in types surface motility (A or adventurous motility) csgA mutant, which unable to form macroscopic travelling waves known as ripples, has demonstrated both systems but not rippling...
Summary Small non‐coding RNAs (sRNAs) are expected to have pivotal roles in the adaptive responses underlying symbiosis of nitrogen‐fixing rhizobia with legumes. Here, we provide primary insights into function and activity mechanism Sinorhizobium meliloti trans ‐sRNA NfeR1 (Nodule Formation Efficiency RNA). Northern blot probing transcription tracking fluorescent promoter‐reporter fusions unveiled high nfeR1 expression response salt stress throughout symbiotic interaction. The strength...
The rhizosphere and rhizoplane are nutrient-rich but selective environments for the root microbiome. Here, we deciphered a posttranscriptional network regulated by homologous trans-small RNAs (sRNAs) AbcR1 AbcR2, which rewire metabolism of nitrogen-fixing α-rhizobium Sinorhizobium meliloti during preinfection stages symbiosis with its legume host alfalfa. LysR-type regulator LsrB, transduces cell redox state, is indispensable expression in actively dividing bacteria, whereas stress-induced...
Abstract Cas13-containing type VI CRISPR-Cas systems specifically target RNA; however, the mechanism of spacer acquisition remains unclear. We have previously reported association reverse transcriptase–Cas1 (RT–Cas1) fusion proteins with certain types VI-A systems. Here, we show that RT–Cas1 are also recruited by VI-B in bacteria from gut microbiomes, constituting a VI-B1 variant system includes CorA-encoding locus addition to CRISPR array and RT–Cas1/Cas2 adaptation module. found RT-CRISPR...
Root nodule endosymbioses between diazotrophic rhizobia and legumes provide the largest input of combined N to biosphere, thus representing an alternative harmful chemical fertilizers for sustainable crop production. Rhizobia have evolved intricate strategies coordinate assimilation their own benefit with