A5073162092- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Catalysis for Biomass Conversion
- Enzyme Catalysis and Immobilization
- Catalysis and Hydrodesulfurization Studies
- Catalytic Processes in Materials Science
- Biocrusts and Microbial Ecology
- Microbial Community Ecology and Physiology
- RNA modifications and cancer
- Metal Extraction and Bioleaching
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Metalloenzymes and iron-sulfur proteins
National and Kapodistrian University of Athens
2021-2023
Biodesulfurization poses as an ideal replacement to the high cost hydrodesulfurization of recalcitrant heterocyclic sulfur compounds, such dibenzothiophene (DBT) and its derivatives. The increasingly stringent limits on fuel content intensify need for improved desulfurization biocatalysts, without sacrificing calorific value fuel. Selective removal in a wide range biodesulfurization strains, well model biocatalyst Rhodococcus qingshengii IGTS8, occurs via 4S metabolic pathway that involves...
Synthetic biology applications rely on a well-characterized set of microbial strains, with an established toolbox molecular methods for their genetic manipulation. Since there are no thermophiles such attributes, most biotechnology and synthetic studies use organisms that grow in the mesophilic temperature range. As result, thermophiles, heterogenous group microbes thrive at high (>50 °C) temperatures, largely overlooked, respect to biotechnological potential, even though they share...
Precise genome editing of the model biocatalyst Rhodococcus qingshengii IGTS8 was performed for first time, more than 3 decades after its initial discovery. We thus gained insight into regulation dsz gene expression and activity, depending on presence two reverse transsulfuration enzymes, CβS MetB.
Xylanases have a broad range of applications in agro-industrial processes. In this study, we report on the discovery and characterization new thermotolerant GH10 xylanase from Bacillus safensis, designated as BsXyn10. The gene (bsxyn10) was cloned safensis expressed Escherichia coli. reduced molecular mass BsXyn10 48 kDa upon SDS-PAGE. Bsxyn10 optimally active at pH 7.0 60 °C, stable over (5.0–8.0), also revealed tolerance toward different modulators (metal cations, EDTA). enzyme various...
Biodesulfurization (BDS) is considered a complementary technology to the traditional hydrodesulfurization treatment for removal of recalcitrant sulfur compounds from petroleum products. BDS was investigated in bubble column bioreactor using two-phase media. The effects various process parameters, such as biocatalyst age and concentration, organic fraction percentage (OFP), type compound—namely, dibenzothiophene (DBT), 4-methyldibenzothiophene (4-MDBT), 4,6-dimethyldibenzothiophene...
Sustainable biodesulfurization (BDS) processes require the use of microbial biocatalysts that display high activity against recalcitrant heterocyclic sulfur compounds and can simultaneously withstand harsh conditions contact with petroleum products, inherent to any industrial biphasic BDS system. In this framework, functional BDS-related diversity in a naturally oil-exposed ecosystem, was examined through 4,6-dimethyl-dibenzothiophene based enrichment process. Two new Rhodococcus sp. strains...
Zinc fingers consist of one the most abundant motifs in transcription factors and DNA-binding proteins. Recent studies provide evidence on pathological implication zinc finger proteins various neurodevelopmental disorders malignancies but their role pediatric brain tumors is largely unexplored. To this end, we investigated differential expression finger-containing genes along with relevant biological processes pathways among four main tumor categories (pilocytic astrocytomas, ependymomas,...
Abstract Biodesulfurization (BDS) is a process that selectively removes sulfur from dibenzothiophene and its derivatives. Several natural biocatalysts have been isolated, all harboring the highly conserved desulfurization operon dszABC . Even though phenotype known to be significantly repressed by methionine, cysteine, inorganic sulfate, available information on metabolic regulation of gene expression still limited. In this study, scarless knockouts metabolism-related cbs metB genes are...
Abstract Biodesulfurization (BDS) is a process that selectively removes sulfur from dibenzothiophene and its derivatives. Several mesophilic natural biocatalysts have been isolated, harboring the highly conserved desulfurization operon dszABC . Even though phenotype known to be significantly repressed by methionine, cysteine, inorganic sulfate, available information on metabolic regulation of gene expression still limited. In this study, scarless knockouts metabolism-related cbs metB genes...