A5083778545- Cellular Mechanics and Interactions
- Muscle Physiology and Disorders
- RNA Research and Splicing
- Cellular transport and secretion
- Developmental Biology and Gene Regulation
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Erythrocyte Function and Pathophysiology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Hippo pathway signaling and YAP/TAZ
- Microtubule and mitosis dynamics
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Cell Adhesion Molecules Research
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Neurobiology and Insect Physiology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Nuclear Structure and Function
- Force Microscopy Techniques and Applications
- Blood properties and coagulation
- Animal Behavior and Reproduction
- Knee injuries and reconstruction techniques
- Cancer Genomics and Diagnostics
- RNA Interference and Gene Delivery
The University of Texas Southwestern Medical Center
2005-2025
Southwestern Medical Center
2017-2024
Stanford University
1997-2020
Johns Hopkins University
2007-2019
Johns Hopkins Medicine
2007-2019
Recent studies in Drosophila have implicated actin cytoskeletal remodeling myoblast fusion, but the cellular mechanisms underlying this process remain poorly understood. Here we show that polymerization occurs an asymmetric and cell type–specific manner between a muscle founder fusion-competent (FCM). In FCM, dense F-actin–enriched focus forms at site of whereas thin sheath F-actin is induced along apposing membrane. The FCM-specific invades with multiple finger-like protrusions, leading to...
Cell-cell fusion is critical for the conception, development, and physiology of multicellular organisms. Although cellular fusogenic proteins actin cytoskeleton are implicated in cell-cell fusion, it remains unclear whether how they coordinate to promote plasma membrane fusion. We reconstituted a high-efficiency, inducible cell culture system normally nonfusing Drosophila S2R+ cells. Both cytoskeletal rearrangements were necessary combination sufficient impart competence. Localized...
Abstract Projection imaging accelerates volumetric interrogation in fluorescence microscopy, but for multi-cellular samples, the resulting images may lack contrast, as many structures and haze are summed up. Here, we demonstrate rapid pro jective light-sheet with p arameter s election (props) of depth, position viewing angle. This allows us to selectively image different sub-volumes a sample, rapidly switch between them exclude background fluorescence. Here power props by functional within...
Significance The formation of skeletal muscle fibers during embryogenesis and adult injury-induced repair occurs through the fusion myoblasts. We recently discovered myomixer, a muscle-specific micropeptide required for myoblast in mice. Myomixer myomaker, another membrane protein, are sufficient to induce nonmuscle cells. Here, we extend these findings demonstrate requirement myomixer zebrafish fusion. also striking functional conservation zebrafish, elephant shark, turtle among other...
ABSTRACT We have investigated the anterior and posterior compartmental organization of genital imaginal disc. Unlike thoracic discs, disc is a compound consisting three primordia – female genital, male anal primordia. Here we provide evidence that each primordium divided into compartments. Genes are known to be expressed in compartment-specific manners other discs (engrailed, hedgehog, patched, decapentaplegic, wingless cubitus interruptus) analogous patterns Specifically, engrailed...
The p21-activated kinases (PAKs) play essential roles in diverse cellular processes and are required for cell proliferation, apoptosis, polarity establishment, migration, shape changes. Here, we have identified a novel function the group I PAKs cell–cell fusion. We show that two Drosophila PAKs, DPak3 DPak1, partially redundant functions myoblast fusion vivo, with playing major role. is enriched at site of colocalizing F-actin focus within podosome-like structure (PLS), promotes actin...
Skeletal muscle regeneration is a multistep process involving the activation, proliferation, differentiation, and fusion of stem cells, known as satellite cells. The s atellite c ell-derived mononucleated m uscle cells (SCMs) indispensable for generation multinucleated, contractile myofibers during repair. However, molecular cellular mechanisms underlying SCM remain poorly understood. In this study, we uncovered an essential role branched actin polymerization in fusion. Using conditional...