A5083484254- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Microtubule and mitosis dynamics
- Skin and Cellular Biology Research
- Cancer Cells and Metastasis
- Antimicrobial agents and applications
- Bacterial biofilms and quorum sensing
- Antimicrobial Peptides and Activities
- Ubiquitin and proteasome pathways
- Photosynthetic Processes and Mechanisms
- Micro and Nano Robotics
- Biopolymer Synthesis and Applications
University of Michigan
2024
Vanderbilt University
2019-2023
Rollins College
2014-2016
Dynamic organization of microtubule minus ends is vital for the formation and maintenance acentrosomal arrays. In vitro, both switch between phases assembly disassembly, a behavior called dynamic instability. Although grow slower, their lifetimes are similar to those plus ends. The mechanisms underlying these distinct dynamics remain unknown. Here, we use an in vitro reconstitution approach investigate minus-end dynamics. We find that not defined by mean size protective GTP-tubulin cap....
Coordination between the microtubule and actin networks is essential for cell motility, neuronal growth cone guidance, wound healing. Members of CLASP (cytoplasmic linker-associated protein) family proteins have been implicated in cytoskeletal cross-talk microtubules networks; however, molecular mechanisms underlying role coordination are unclear. Here, we investigate CLASP2α's cross-linking function with F-actin. Our results demonstrate that CLASP2α cross-links F-actin to lattice vitro. We...
Chlorhexidine is a widely used, commercially available cationic antiseptic. Although its mechanism of action on planktonic bacteria has been well explored, far fewer studies have examined interaction with an established biofilm. The physical effects chlorhexidine biofilm are particularly unknown. Here, the authors report first observations chlorhexidine-induced elastic and adhesive changes to single cells within consistent proposed chlorhexidine. Atomic force microscopy spectroscopy...
Abstract While it is known that cells with differential adhesion tend to segregate and preferentially sort, the physical forces governing sorting invasion in heterogeneous tumors remain poorly understood. To investigate this, we tune matrix confinement, mimicking changes stiffness confinement of tumor microenvironment, explore how influences individual collective cell migration 3D spheroids. High levels lead while reducing triggers fluidization motion. Cell sorting, which depends on...
ABSTRACT Coordination between the microtubule and actin networks is essential for cell motility, neuronal growth cone guidance, wound healing. Members of CLASP (Cytoplasmic Linker-Associated Protein) family proteins have been implicated in cytoskeletal crosstalk microtubules networks, however, molecular mechanisms underlying CLASPs role coordination are unclear. Here, we investigate CLASP2α’s crosslinking function with F-actin. Our results demonstrate that CLASP2α crosslinks F-actin to...