A5051080339- Hepatitis C virus research
- Immune Cell Function and Interaction
- Galectins and Cancer Biology
- Vaccine Coverage and Hesitancy
- Hepatitis B Virus Studies
- SARS-CoV-2 and COVID-19 Research
- Liver Disease Diagnosis and Treatment
- HIV Research and Treatment
- Systemic Lupus Erythematosus Research
- Phagocytosis and Immune Regulation
- T-cell and B-cell Immunology
- HIV/AIDS drug development and treatment
- Viral Infections and Immunology Research
- Monoclonal and Polyclonal Antibodies Research
- Cytomegalovirus and herpesvirus research
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Infection Control and Ventilation
- Endoplasmic Reticulum Stress and Disease
- Herpesvirus Infections and Treatments
Medical University of Warsaw
2018-2025
Abstract Identification of pathogens causing viral encephalitis remains challenging, and in over 50% cases the etiologic factor undetermined. Next-generation sequencing (NGS) based metagenomics has been successfully used to detect novel rare infections, but its value for routine diagnosis unclear. The aim present study was determine sensitivity shotgun metagenomic protocols, which include preamplification, testing it against cerebrospinal fluid (CSF) samples from patients. For HIV HBV...
Introduction: Healthcare workers in Poland received a booster dose of the BNT162b2 mRNA vaccine (Pfizer-BioNTech, Manufacturer: Pfizer, Inc., and BioNTech; Moguncja, Germany) at beginning October 2021. Here, we report on preliminary results an ongoing clinical study into antibody response to SARS-CoV-2 healthcare previously exposed virus, with or without evidence past infection, Hospital for Infectious Diseases Warsaw before after dose. Methods: Blood samples were collected day was...
During chronic hepatitis C virus (HCV) infection, both CD4+ and CD8+ T-cells become functionally exhausted, which is reflected by increased expression of programmed cell death-1 (PD-1) T-cell immunoglobulin mucin domain-containing protein 3 (Tim-3), elevated anti-inflammatory interleukin 10 (IL-10) plasma levels. We studied 76 DAA-treated HCV-positive patients 18 non-infected controls. Flow cytometry measured pretreatment frequencies CD4+PD-1+, CD4+PD-1+Tim-3+ CD8+PD-1+Tim-3+ IL-10 levels...
Abstract Background T-cell responses during chronic viral infections become exhausted, which is reflected by upregulation of inhibitory receptors (iRs) and increased interleukin 10 (IL-10). We assessed 2 iRs—PD-1 (programmed cell death protein 1) Tim-3 (T-cell immunoglobulin mucin domain–containing 3)—and IL-10 mRNAs in peripheral blood mononuclear cells (PBMCs) their soluble analogs (sPD-1, sTim-3, IL-10) plasma HIV-1/hepatitis C virus (HCV) coinfection explored the effect HCV treatment on...
Most Hepatitis C virus (HCV)-infected subjects develop chronic infection, whereas a minority clear the in early phase of infection. We analyzed factors associated with outcome (chronicity vs clearance) during preclinical seronegative community-acquired HCV Among 17.5 million blood donations years 2000-2016, 124 donors were found to be RNA-positive/anti-HCV-negative. All contacted after 0.5-12.7 and 40 responded provided sample. Hypervariable region 1 was by ultradeep pyrosequencing cytokines...
Chronic hepatitis C virus (HCV) infection is characterized by increased proportion of CD4+CD8+ double positive (DP) T cells, but their role in this unclear. In chronic C, immune responses to HCV become functionally exhausted, which manifests itself expression programmed cell death protein 1 (PD-1) and T-cell immunoglobulin- mucin-domain-containing molecule-3 (Tim-3) on cells. The aim our study was determine PD-1 Tim-3 phenotype DP cells subjects with naturally resolved infection. Peripheral...
During chronic hepatitis C virus (HCV) infection, CD8+ T-cells become functionally exhausted, undergoing progressive phenotypic changes, i.e., overexpression of "inhibitory" molecules such as PD-1 (programmed cell death protein 1) and/or Tim-3 (T-cell immunoglobulin and mucin domain-containing molecule-3). The extreme intrahost genetic diversity HCV is a major mechanism immune system evasion, facilitating epitope escape. aim the present study was to determine whether T-cell exhaustion...
In chronic hepatitis C (CHC), characterized by exhaustion of T-cell function, increased frequencies double-positive (DP) (CD4+CD8+) cells are present in peripheral blood. We compared the phenotype between DP and single positive (SP) T-cells, including HCV-specific cells, assessed effect successful HCV treatment on inhibitory receptors expression. Blood samples from 97 CHC patients were collected before six months post-treatment. PD-1 (programmed cell death protein 1) Tim-3 (T-cell...
Molecular characterization of early hepatitis C virus (HCV) infection remains rare. Ten out 78 patients a hematology/oncology center were found to be HCV RNA positive two four months after hospitalization. Only the ten anti-HCV positive. hypervariable region 1 (HVR1) was amplified in seven (including one positive) and analyzed by next generation sequencing (NGS). Genetic variants reconstructed Shorah an empirically established 0.5% variant frequency cut-off implemented. These sequences...
Abstract Hepatitis C virus (HCV) is characterized by high genetic variability, which manifested both at the inter-host and intra-host levels. However, its role in clinical course of infection less obvious. The aim present study was to determine variability HCV HVR1 (hypervariable region 1) genotype 1b 3 plasma blood donors early seronegative stage (HCV-RNA+, anti-HCV−) samples from chronically infected patients using next-generation sequencing. Sequencing errors were corrected, haplotypes...
Introduction: The BNT162b2 vaccination studies did not specifically focus on groups that were heavily exposed to SARS-CoV-2 infection. Therefore, we aimed assess the safety and efficacy of vaccine among healthcare workers (HCWs). Methods: Study participants recruited from hospital employees who received at Hospital for Infectious Diseases in Warsaw. Blood samples collected before after each dose. At timepoint, levels anti-SARS CoV-2 IgM, anti-n IgG, S-RBD antibodies measured. Data...