A5046212535- Hedgehog Signaling Pathway Studies
- Oral and gingival health research
- HIV Research and Treatment
- Immune Cell Function and Interaction
- Tumors and Oncological Cases
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- COVID-19 Clinical Research Studies
- Vascular Malformations and Hemangiomas
- Genetic and rare skin diseases.
- Cancer and Skin Lesions
- HIV/AIDS drug development and treatment
- Chromatin Remodeling and Cancer
- Genomic variations and chromosomal abnormalities
- Dupuytren's Contracture and Treatments
- Long-Term Effects of COVID-19
- Advanced biosensing and bioanalysis techniques
- Animal Virus Infections Studies
- T-cell and B-cell Immunology
- Virus-based gene therapy research
- Nanowire Synthesis and Applications
- HIV-related health complications and treatments
- Immune Response and Inflammation
- Immune cells in cancer
- Hepatitis C virus research
Hospital Universitario Virgen del Rocío
2019-2024
Instituto de Biomedicina de Sevilla
2019-2024
Universidad de Sevilla
2019-2024
Purpose CAR-T cell therapy has proven to be a disruptive treatment in the hematology field, however, less than 50% of patients maintain long-term response and early predictors outcome are still inconsistently defined. Here, we aimed optimize detection CD19 cells blood identify phenotypic features as biomarkers associated with toxicity outcomes. Experimental design In this study, monitoring by flow cytometry digital PCR (dPCR), immunophenotypic characterization circulating from 48 treated...
Background: Persistent controllers (PC) maintain antiretroviral-free HIV-1 control indefinitely over time while transient (TC) eventually lose virological control. It is essential to characterize the quality of HIV reservoir these phenotypes identify factors that lead progression and open new avenues in cure strategies. Methods: The characterization reservoir, from peripheral blood mononuclear cells, was performed using next-generation sequencing techniques, such as full-length individual...
The immune factors associated with impaired SARS-CoV-2 vaccine response in elderly people are mostly unknown. We studied individuals older than 60 and younger years, who had been vaccinated BNT162b2 mRNA, before after the first second dose. Aging was a lower anti–RBD IgG levels decreased magnitude polyfunctionality of SARS-CoV-2–specific T cell response. dramatic decrease thymic function > which fueled alteration homeostasis, their CD161+ were 2 months vaccination. Additionally, deficient DC...
Plasmacytoid dendritic cells (pDCs) sense viral and bacterial products through Toll-like receptor (TLR)-7 -9 translate this sensing into Interferon-α (IFN-α) production T-cell activation. The understanding of the mechanisms involved in pDCs stimulation may contribute to HIV-cure immunotherapeutic strategies. objective present study was characterize immunomodulatory effects TLR agonist stimulations several HIV-1 disease progression phenotypes non infected donors.pDCs, CD4 CD8 T-cells were...
Abstract SARS‐CoV‐2 specific T‐cell response has been associated with disease severity, immune memory and heterologous to endemic coronaviruses. However, an integrative approach combining a comprehensive analysis of the quality antibody levels in these three scenarios is needed. In present study, we found that, acute infection, while mild was high polyfunctionality biased IL‐2 production inversely correlated anti‐S IgG levels, combinations only including IFN‐γ absence perforin predominated...
We evaluated the safety and viral rebound, after analytical treatment interruption (ATI), of vedolizumab ART in recent HIV-1 infection. used this model to analyze impact α4β7 on reservoir size. Participants started with monthly Vedolizumab infusions ATI was performed at week 24. Biopsies were obtained from ileum caecum baseline levels, reservoir, flow cytometry cell-sorting antibody competition experiments assayed. safe well-tolerated. No participant achieved undetectable viremia off 24...
Some HIV controllers experience immunologic progression with CD4+ T cell decline. We aimed to identify genetic factors associated lost in controllers. A total of 561 were included, 442 and 119 from the International Study Cohort Swiss Study, respectively. No SNP or gene was long-term non-progressor spontaneous control phenotype individual GWAS meta-analysis. However, SNPs previously natural linked HLA-B (rs2395029 [p = 0.005; OR 1.70], rs59440261 0.003; 1.78]), MICA (rs112243036 0.011;...
The activation phenotypes and functional changes in monocyte subsets during hepatitis C virus (HCV) elimination HIV/HCV-coinfected patients were evaluated. Twenty-two on suppressive combination antiretroviral treatment (cART) achieving HCV after direct-acting antiviral (DAA) therapy 10 HIV-monoinfected included. phenotype (10 markers) polyfunctionality (intracellular interleukin-1α [IL-1α], IL-1β, IL-6, IL-8, tumor necrosis factor alpha [TNF-α], IL-10 production) three (classical,...
Sclerotic chronic GVHD (scGVHD) is characterized by progressive skin fibrosis and frequent refractoriness to available therapies. Aberrant activation of Hedgehog signaling in dermal fibroblasts has been implicated scGVHD. Here, we report the results two phase I/II studies (NCT03415867, GETH-TC; NCT04111497, FHD) that evaluated glasdegib, a smoothened antagonist, as novel therapeutic agent refractory scGVHD.Adult patients with active scGVHD after ≥1 (FHD) or ≥2 (GETH-TC) lines therapy were...
Human CD300a is known to promote the infection by dengue and other enveloped viruses overexpressed on CD4 + T cells from HIV-1-infected patients. We found that infected CD4+RA− untreated patients were mostly CD300a+. Furthermore, expressing healthy donors significantly more HIV-1 in vitro than CD300a− cells. might represent a biomarker of susceptibility memory lymphocytes.
SUMMARY The immune factors associated with impaired SARS-CoV-2 vaccine response in the elderly are mostly unknown. We studied old and young people vaccinated BNT162b2 mRNA before after first second dose. Aging was a lower anti-RBD IgG levels decreased magnitude polyfunctionality of specific T cell response. dramatic decrease thymic function elderly, which fueled alteration homeostasis, CD161+ were two months vaccination. Additionally, deficient dendritic (DC) homing, activation Toll like...
ABSTRACT SARS-CoV-2 specific T-cell response has been associated with disease severity, immune memory and heterologous to endemic coronaviruses. However, an integrative approach combining a comprehensive analysis of the quality antibody levels in these three scenarios is needed. In present study we found that, acute infection, while mild was high polyfunctionality biased IL-2 production inversely correlated anti-S IgG levels, combinations only including IFN-γ absence perforin predominated...
<p>Fold change from baseline in the absolute count of circulating B cells (C1D1: n=13 responders [R], n=6 non-responders [NR]; C2D1: R, NR; C4D1: n=12 n=4 NR) (A), naive n=11 n=5 (B), unswitched memory n=3 (C) and switched (D). E) Fold CD268 (BAFF) expression on NR). MFI normalised ratios were used for quantification. MFI, median fluorescence intensity; n.s., non-statistically significant.</p>
<p>Expression of T-cell co-stimulatory and co-inhibitory molecules. Expression CTLA-4, TNFRSF18, PD-1, OX40 4-IBB in the CD4+ CD8+ compartments was measured by flow cytometry under resting conditions after 4-hour incubation with ionomycin (0.91 µg/mL) PMA (20 µg/mL). Brefeldin A (10 μg/mL) added both conditions. Differences between serial evaluations on day 1 cycles 1, 2 4 were examined skin/joints scGVHD responder (C1D1: n=11; C2D1: n=13; C4D1: n=12) non-responder patients n=7; n=5)....
<p>Phosphoflow analysis of T-cell activation signaling pathways. Flow cytometric measurements ERK (pT202/204), p38 (pT180/y182), STAT3 (pY705), and STAT5 (pY694) phosphorylation were performed at baseline after with PMA 40 nM (pERK analyses), IL-2 50 ng/mL (pSTAT5 analysis) or IL-6 20 (pSTAT3 in the CD4+ CD8+ subsets. Differences between post-activation/resting MFI ratios examined skin/joints scGVHD responder (C1D1: n=13; C2D1: C4D1: n=12) non-responder n=7; n=5) patients serial...
<div>AbstractPurpose:<p>Sclerotic chronic GVHD (scGVHD) is characterized by progressive skin fibrosis and frequent refractoriness to available therapies. Aberrant activation of Hedgehog signaling in dermal fibroblasts has been implicated scGVHD. Here, we report the results two phase I/II studies (NCT03415867, GETH-TC; NCT04111497, FHD) that evaluated glasdegib, a smoothened antagonist, as novel therapeutic agent refractory scGVHD.</p>Patients Methods:<p>Adult patients...
<p>Phosphoflow analysis of T-cell activation signaling pathways. Flow cytometric measurements ERK (pT202/204), p38 (pT180/y182), STAT3 (pY705), and STAT5 (pY694) phosphorylation were performed at baseline after with PMA 40 nM (pERK analyses), IL-2 50 ng/mL (pSTAT5 analysis) or IL-6 20 (pSTAT3 in the CD4+ CD8+ subsets. Differences between post-activation/resting MFI ratios examined skin/joints scGVHD responder (C1D1: n=13; C2D1: C4D1: n=12) non-responder n=7; n=5) patients serial...