A5050643824- Connexins and lens biology
- Pancreatic function and diabetes
- Heat shock proteins research
- Nicotinic Acetylcholine Receptors Study
- Heme Oxygenase-1 and Carbon Monoxide
- Thermoregulation and physiological responses
- Redox biology and oxidative stress
- Bladder and Urothelial Cancer Treatments
- Coronary Interventions and Diagnostics
- Neuroscience of respiration and sleep
- Immunotherapy and Immune Responses
- Diabetes and associated disorders
- Melanoma and MAPK Pathways
- Genetics and Neurodevelopmental Disorders
- Peripheral Artery Disease Management
- Adipose Tissue and Metabolism
- Complement system in diseases
- Metabolism, Diabetes, and Cancer
- Macrophage Migration Inhibitory Factor
- Endoplasmic Reticulum Stress and Disease
- Cell death mechanisms and regulation
- Hormonal Regulation and Hypertension
- Protein Kinase Regulation and GTPase Signaling
- Cancer Immunotherapy and Biomarkers
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
University of Lausanne
2013-2023
University Hospital of Lausanne
2011-2020
University of Geneva
1997-2017
Inserm
2014-2017
Centre de Recherche Cardio-Thoracique de Bordeaux
2017
Université de Bordeaux
2017
Hôpital Orthopédique de la Suisse Romande
2012-2016
Hôpital Saint-Jacques
2016
Collège de France
2014
University of Regensburg
2014
The cellular distribution of connexin40 (Cx40), a newly cloned gap junction structural protein, was examined by immunofluorescence microscopy using two different specific anti-peptide antibodies. Cx40 detected in the endothelium muscular as well elastic arteries punctate pattern consistent with known junctions. However, it not other cells vascular wall. By contrast, Cx43, another connexin present cardiovascular system, endothelial but abundant myocardium and aortic smooth muscle. We have...
We have used low stringency hybridization and polymerase chain reaction (PCR) amplification with degenerate oligonucleotides to identify four new members of the rat connexin gene family. On basis their predicted molecular mass, these proteins been designated (Cx) 40 (Cx40), Cx37, Cx33, Cx31.1. The connexins exhibit all conserved structural features family, including highly similar extracellular transmembrane domains but divergent major cytoplasmic domains. primary sequence similarity, family...
The expression of Staphylococcus aureus adhesins in Lactococcus lactis identified clumping factor A (ClfA) and fibronectin-binding protein (FnBPA) as critical for valve colonization rats with experimental endocarditis. This study further analyzed their role disease evolution. Infected animals were followed 3 d. ClfA-positive lactococci successfully colonized damaged valves, but spontaneously eradicated over 48 h. In contrast, FnBPA-positive progressively increased bacterial titers...
Macrophage migration inhibitory factor (MIF), originally identified as a cytokine secreted by T lymphocytes, was found recently to be both pituitary hormone and mediator released immune cells in response glucocorticoid stimulation. We report here that the insulin-secreting β cell of islets Langerhans expresses MIF its production is regulated glucose time- concentration-dependent manner. insulin colocalize immunocytochemistry within secretory granules pancreatic islet cells, once released,...
Diabetes is associated with significant changes in plasma concentrations of lipoproteins. We tested the hypothesis that lipoproteins modulate function and survival insulin-secreting cells. first detected presence several receptors participate binding processing confirmed internalization fluorescent low density lipoprotein (LDL) high (HDL) particles β-cells. Purified human very (VLDL) LDL reduced insulin mRNA levels β-cell proliferation induced a dose-dependent increase rate apoptosis. In...
ABSTRACT Since Staphylococcus aureus expresses multiple pathogenic factors, studying their individual roles in single-gene-knockout mutants is difficult. To circumvent this problem, S. clumping factor A ( clfA ) and fibronectin-binding protein fnbA genes were constitutively expressed poorly Lactococcus lactis using the recently described pOri23 vector. The recombinant organisms tested vitro for adherence to immobilized fibrinogen fibronectin vivo ability infect rats with catheter-induced...
Previous studies have provided evidence for the transcripts of Cx43 and Cx45 within pancreatic islets. As yet, however, it has proven difficult to unambiguously demonstrate expression these proteins by islet cells. We investigated whether Cx36, a new connexin species recently identified in mammalian brain retina, may also be expressed Using probes that permitted original identification Cx36 central nervous system, we show transcript is clearly detectable rat novel affinity-purified...
Journal Article Precursor-product relationship between vitellogenin and the yolk proteins as derived from complete sequence of a Xenopus gene Get access Susan Gerber-Huber, Gerber-Huber Institut de Biologie Animale, Université Lausanne, Båtimem BiologieCH-1015 Lausanne Search for other works by this author on: Oxford Academic PubMed Google Scholar Denise Nardelli, Nardelli Jacques-Antoine Haefliger, Haefliger David N. Cooper, Cooper Françoise Givel, Givel Jacques-Edouard Germond, Germond...
Complement C6 is one of five plasma proteins that are incorporated into the lytic terminal complement complex on lipid membranes (C5b-9m) upon activation cascade. Oligonucleotide probes derived from partial amino acid sequences purified were used to isolate cDNA clones a human liver library. The complete polypeptide structure mature deduced sequence consists 913 residues preceded by typical 21-residue signal peptide. most similar in C7, sharing 33.5% identical with C7 including all 56...
Connexins (Cxs) play a role in the contractility of aorta wall. We investigated how connexins endothelial cells (ECs; Cx37, Cx40) and smooth muscle (SMCs; Cx43, Cx45) change during renin-dependent -independent hypertension. subjected both wild-type (WT) mice lacking Cx40 (Cx40−/−), to either two-kidney, one-clip procedure or N-nitro-l-arginine-methyl-ester treatment, which induce hypertension, respectively. All hypertensive featured thickened aortic wall, increased levels Cx37 Cx45 SMC, EC...
ABSTRACT Staphylococcus aureus harbors redundant adhesins mediating tissue colonization and infection. To evaluate their intrinsic role outside of the staphylococcal background, a system was designed to express them in Lactococcus lactis subsp. cremoris 1363. This bacterium is devoid virulence factors has known genetic background. A new Escherichia coli-L. shuttle expression vector constructed for this purpose. First, high-copy-number lactococcal plasmid pIL253 equipped with oriCol E1...
To investigate the function of Cx43 during hypertension, we studied mouse line Cx43KI32 (KI32), in which coding region Cx32 replaces that Cx43. Within kidneys homozygous KI32 mice, was expressed cortical and medullary tubules, as well some extra- intraglomerular vessels, i.e., at sites where are found WT mice. Under such conditions, renin expression much reduced compared with observed heterozygous littermates. After exposure to a high-salt diet, all mice retained normal blood pressure....
No AccessJournal of Urology1 Nov 1991A Multicenter Randomized Trial Comparing the Luteinizing Hormone-Releasing Hormone Analogue Goserelin Acetate Alone and with Flutamide in Treatment Advanced Prostate Cancer C.J. Tyrrell, J.E. Altwein, F. Klippel, E. Varenhorst, G. Lunglmayr, Boccardo, I.M. Holdaway, J.-M. Haefliger, J.P. Jordaan, M. Sotarauta, International Study Group† TyrrellC.J. Tyrrell More articles by this author , AltweinJ.E. Altwein KlippelF. Klippel VarenhorstE. Varenhorst...
Cytoplasmic granules of cytolytic T lymphocytes contain at least six related serine esterases (granzymes) that are released together with perforin, a pore-forming protein to complement component C9, during target-cell lysis. Polyclonal antibodies were used isolate large number cDNA clones from an expression library derived cytolytic-T-cell mRNA. Three distinct full-length coding for granzymes D, E, and F identified by restriction site mapping nucleotide sequencing. The three deduced amino...
Upon agonist stimulation, endothelial cells trigger smooth muscle relaxation through the release of relaxing factors such as nitric oxide (NO). Endothelial mouse aorta are interconnected by gap junctions made connexin40 (Cx40) and connexin37 (Cx37), allowing exchange signaling molecules to coordinate their activity. Wild-type (Cx40(+/+)) hypertensive Cx40-deficient mice (Cx40(-/-)), which also exhibit a marked decrease Cx37 in endothelium, were used investigate link between expression...
Type 1 diabetes develops when most insulin-producing β cells of the pancreas are killed by an autoimmune attack. The in vivo conditions modulating sensitivity and resistance to this attack remain largely obscure. Here, we show that connexin 36 (Cx36), a trans-membrane protein forms gap junctions between pancreatic islets, protects mouse against both cytotoxic drugs cytokines prevail islet environment at onset type diabetes. We documented protection was least partially dependent on...