A5006695630- Adrenal Hormones and Disorders
- Stress Responses and Cortisol
- Hormonal and reproductive studies
- Hormonal Regulation and Hypertension
- Monoclonal and Polyclonal Antibodies Research
- Vitamin D Research Studies
- Exercise and Physiological Responses
- Estrogen and related hormone effects
- Vitamin C and Antioxidants Research
- Glycosylation and Glycoproteins Research
- Pituitary Gland Disorders and Treatments
- Sepsis Diagnosis and Treatment
- Pharmacological Effects and Assays
- Hypothalamic control of reproductive hormones
- Receptor Mechanisms and Signaling
- Sports injuries and prevention
- Blood groups and transfusion
- Fungal Infections and Studies
- Thyroid Disorders and Treatments
- Birth, Development, and Health
- Cardiovascular Disease and Adiposity
- Protein purification and stability
- Sports Performance and Training
- Antifungal resistance and susceptibility
- Electrolyte and hormonal disorders
Canterbury Health Laboratories
2014-2024
University of Otago
1987-2024
Christchurch Hospital
1985-2009
Canterbury District Health Board
2009
University of New Mexico
2007
Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit
2007
Norwich Research Park
2002
Gilead Sciences (United States)
1996
Johns Hopkins University
1989-1992
Johns Hopkins Medicine
1989-1991
There is a paucity of longitudinal data on plasma and urinary cortisol levels during pregnancy using modern assays. Furthermore, conflicting exist as to the effect low-dose oral contraceptive pill (OCP) cortisol. DESIGN, SUBJECTS, AND MEASUREMENTS: We conducted prospective study morning (total free), corticosteroid-binding globulin (CBG), 24-h free (UFC) in 20 pregnant women first, second, third trimesters 2-3 months postpartum compared with 12 subjects OCP 15 nonpregnant not taking (control...
Context: Severe systemic infection leads to hypercortisolism. Reduced cortisol binding proteins may accentuate the free elevations seen in infection. Recently, low total increments after tetracosactrin have been associated with increased mortality and hemodynamic responsiveness exogenous hydrocortisone septic shock (SS), a phenomenon termed by some investigators as relative adrenal insufficiency (RAI). Hypothesis: Free plasma correspond more closely illness severity than cortisol, comparing...
Development of antisense technology has focused in part on creating improved methods for delivering oligodeoxynucleotides (ODNs) to cells. In this report, we describe a cationic lipid that, when formulated with the fusogenic dioleoylphosphatidyliethanolamine, greatly improves cellular uptake properties ODNs, as well plasmid DNA. This formulation, termed GS 2888 cytofectin, (i) efficiently transfects ODNs and plasmids into many cell types presence or absence 10% serum medium, (ii) uses 4-...
Journal Article DIFFUSE INTERSTITIAL PULMONARY FIBROSIS: A Clinical, radiological, and pathological study based on 45 patients Get access J.L. LIVINGSTONE, LIVINGSTONE From the Brompton Hospital Institute of Diseases Chest Search for other works by this author on: Oxford Academic PubMed Google Scholar J.G. LEWIS, LEWIS LYNNE REID, REID K.E. JEFFERSON QJM: An International Medicine, Volume 33, Issue 1, January 1964, Pages 71–103, https://doi.org/10.1093/oxfordjournals.qjmed.a067014 Published:...
Glucocorticoid replacement is essential in patients with primary and secondary adrenal insufficiency, but many remain on higher than recommended dose regimens. There no uniformly accepted method to monitor the individual patients. We have compared cortisol concentrations plasma, saliva urine achieved following "physiological" "stress" doses of hydrocortisone as potential methods for monitoring glucocorticoid replacement. Cortisol profiles were measured (20 mg oral) or (50 intravenous)...
Abstract Thirteen laboratories participated in an interlaboratory method performance (collaborative) study on a for the determination of arsenic, cadmium, mercury, and lead by inductively coupled plasma/mass spectrometry (ICP/MS) after pressure digestion including microwave heating technique. Prior to study, were able practice samples with defined element levels (pretrial test). The was tested total 7 foodstuffs: carrot puree, fish muscle, mushroom, graham flour, simulated diet, scampi,...
Abstract Rugby union is a physically intense intermittent sport coupled with high force collisions. Each position within team has specific requirements which are typically based on speed, size and skill. The aim of this study was to investigate the contemporary demands each whether they can explain changes in psychophysiological stress. Urine saliva samples were collected before after five selected Super 15 rugby games from 37 players. Total neopterin (NP), cortisol immunoglobulin A analysed...
Combative sport is one of the most physically intense forms exercise, yet effect recovery interventions has been largely unexplored. We investigated cold-water immersion on structural, inflammatory, and physiological stress biomarkers following a mixed martial arts (MMA) contest preparation training session in comparison with passive recovery. Semiprofessional MMA competitors (n = 15) were randomly assigned to (15 min at 10 °C) or protocol (ambient air) completed immediately session. Markers...
AbstractRugby union is a sport governed by the impacts of high force and frequency. Analysis physiological markers following game can provide an understanding response individual time course changes in to recovery.Urine saliva were collected from 11 elite amateur rugby players 24 h before, immediately after, at 17, 25, 38, 62 86 post-game. Myoglobin, salivary immunoglobulin A cortisol analysed ELISA, whereas neopterin total high-performance liquid chromatography.There was significant...
Journal Article GOUT AND THE SERUM URIC ACID IN DIABETES MELLITUS Get access A. GORDON BECKETT, BECKETT Diabetic Department, Royal Free HospitalLondon Search for other works by this author on: Oxford Academic PubMed Google Scholar J. G. LEWIS QJM: An International of Medicine, Volume 29, Issue 3, July 1960, Pages 443–458, https://doi.org/10.1093/oxfordjournals.qjmed.a066885 Published: 01 1960 history Received: 07 August 1959
We have constructed a genetic linkage map of the long arm human chromosome 21 comprising 27 DNA markers. This is an updated version that reported earlier by group (1989, Genomics 4: 579-591), which contained 17 The current markers consist 10 genes and anonymous sequences. Traditional methods (restriction fragment length polymorphisms) were used to 25 these markers, whereas 2 studied polymerase chain reaction amplification (GT)n dinucleotide repeats. Linkage analysis was performed on 40 CEPH...
To measure and contrast maternal cortisol corticosteroid-binding globulin (CBG) levels in pregnancies with normal outcomes, pre-eclampsia, intrauterine growth restriction (IUGR) gamete recipients.Prospective study of 93 women at high risk including recipients (n = 22) 33 controls. Plasma total free CBG were measured every 2 weeks from 16 weeks' gestation until delivery.Forty-two per cent the high-risk group had complications, pre-eclampsia 11), gestational hypertension 16)...
Serum 5alpha-dihydrotestosterone (DHT) and testosterone (T) were measured in 98 normal adult men aged between 20-80 years, separating T DHT by thin layer chromatography using a sensitive reliable radio-immunoassay. In three age groups 20-40, 40-60 60-80, the means +/- SEM for serum 84 4, 79 3 67 (ng/100 ml) respectively. The corresponding values 559 25, 491 25 475 28 ml). A significant decrease was observed level of 60-80 years group compared to either 20-40 (P less than 0.01) or 0.02)...
Corticosteroid-binding globulin (CBG) is cleaved by neutrophil elastase converting the high-affinity (haCBG) conformation of CBG to a low-affinity (laCBG) with ninefold reduced cortisol-binding affinity. These in vitro data suggest that cortisol release cleavage results targeted delivery areas inflammation. Our objective was determine whether alters circulating levels haCBG and laCBG vivo proportion sepsis severity.Prospective, observational cohort study an adult tertiary level Intensive...
An enzyme-linked immunosorbent assay is described for the measurement of ouabain in human plasma. This specific ouabain, strophanthidin, and ouabagenin, with other steroids, including digoxin vasopressor hormones, exhibiting negligible cross-reactivity. Assay sensitivity was 0.06 nmol/L if 1 mL plasma extracted less than 0.005 when 20 analyzed. Extracted samples showed ouabainlike immunoreactivity that diluted parallel standard curve. Repeated extraction single samples, however, did not...