A5024749463- 3D Printing in Biomedical Research
- Neuroscience and Neural Engineering
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- Microfluidic and Bio-sensing Technologies
- Additive Manufacturing and 3D Printing Technologies
- Analytical Chemistry and Sensors
- Advanced Sensor and Energy Harvesting Materials
- Analog and Mixed-Signal Circuit Design
- Cellular Mechanics and Interactions
- Single-cell and spatial transcriptomics
- Bone Tissue Engineering Materials
- Advanced Materials and Mechanics
- Muscle activation and electromyography studies
- CCD and CMOS Imaging Sensors
- Plant Molecular Biology Research
- Congenital heart defects research
- Nanopore and Nanochannel Transport Studies
- Neurogenesis and neuroplasticity mechanisms
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Advanced Fluorescence Microscopy Techniques
- Zebrafish Biomedical Research Applications
- Skin Protection and Aging
Binghamton University
2018-2025
Gladstone Institutes
2015-2020
Worcester Polytechnic Institute
2012-2018
University of California, San Francisco
2017
Georgia Institute of Technology
2013-2015
The Wallace H. Coulter Department of Biomedical Engineering
2013-2014
Cardiomyocytes derived from human pluripotent stem cells (hPSCs) are a promising cell source for regenerative medicine, disease modeling, and drug discovery, all of which require enriched cardiomyocytes, ideally ones with mature phenotypes. However, current methods typically performed in 2D environments that produce immature cardiomyocytes within heterogeneous populations. Here, we generated 3D aggregates (cardiospheres) differentiation cultures hPSCs using microscale technology rotary...
In this paper, we present a fully integrated multi-modality CMOS cellular sensor array with four sensing modalities to characterize different cell physiological responses, including extracellular voltage recording, impedance mapping, optical detection shadow imaging and bioluminescence sensing, thermal monitoring. The consists of nine parallel pixel groups corresponding signal conditioning blocks. Each group comprises one temperature 16 tri-modality pixels, while each can be independently...
Significance Spinal cord injury (SCI) significantly disrupts normal neural circuitry, leading to severe degradation of motor and sensory function. Excitatory interneurons that relay signals from the brain networks throughout spinal cord, including glutamatergic V2a coordinate respiration locomotion, are lost after SCI. Thus, transplantation SCI could provide a novel therapy restore functional connections between spared downstream neurons. This study describes generation human pluripotent...
Abstract The field of soft wearable bioelectronics requires materials that are flexible, stretchable, biocompatible, and capable being used over long durations. Although polydimethylsiloxane (PDMS) is one the most commonly substrates for these devices due to its biomimetic properties compared biological tissues, intrinsic hydrophobicity causes it underperform in environments. In this work, a hydrophilic, stretchable PDMS electrospun fibrous mat developed overcome limitation by incorporating...
Understanding the relationship between parenchymal and supporting cell populations is paramount to recapitulate multicellular complexity of native tissues. Incorporation stromal cells widely recognized be necessary for stable formation stem cell-derived cardiac tissues; yet, types used have varied widely. This study systematically characterized several found that phenotype morphology was highly variable depending on source exerted differential impacts tissue function induced pluripotent...
Abstract In this study, we created self‐assembled smooth muscle cell (SMC) tissue rings (comprised entirely of cells and cell‐derived matrix; CDM) compared their structure material properties with from SMC‐seeded fibrin or collagen gels. All were cultured statically for 7 days in supplemented growth medium (with ε‐amino caproic acid, ascorbic insulin‐transferrin‐selenium), prior to uniaxial tensile testing histology. Self‐assembled CDM exhibited ultimate strength stiffness values that...
Engineered tissues are being used clinically for tissue repair and replacement, developed as tools drug screening human disease modeling. Self-assembled offer advantages over scaffold-based engineering, such enhanced matrix deposition, strength, function. However, there few available methods fabricating 3D without seeding cells on or within a supporting scaffold. Previously, we system self-assembled rings by into non-adhesive agarose wells. A polydimethylsiloxane (PDMS) negative was first...
Tissue-engineered human blood vessels may enable in vitro disease modeling and drug screening to accelerate advances vascular medicine. Existing methods for tissue-engineered vessel (TEBV) fabrication create homogenous tubes not conducive the focal pathologies characteristic of certain diseases. We developed a system generating self-assembled smooth muscle cell (SMC) ring units, which were fused together into TEBVs. The goal this study was assess feasibility modular assembly fusion building...
Gene delivery to primary human cells is a technology of critical interest both life science research and therapeutic applications. However, poor efficiencies in gene transfer undesirable safety profiles remain key limitations advancing this technology. Here, we describe materials-based approach whereby application bioresorbable mineral coating improves microparticle-based transfection plasmid DNA lipoplexes several cell types. In the presence these mineral-coated microparticles (MCMs),...
Cell-based assays are powerful tools to characterize cell- or tissue-specific physiological behaviors under external biochemical stimuli. External stimuli trigger endogenous cellular mechanisms that produce a cascade of changes, resulting in easily measurable signals. widely used for large-scale drug screening the pharmaceutical industry, where vitro cultured cells potency and toxicity thousands chemicals, leading new development. This is particularly relevant individualized medicine as...
Native cardiac tissue is composed of heterogeneous cell populations that work cooperatively for proper function; thus, engineered models have moved toward incorporating multiple types in an effort to recapitulate native multicellular composition and organization. Cardiac stem cell-derived cardiomyocytes (CMs) require inclusion non-myocytes promote stable formation, yet the specific contributions supporting non-myocyte population on parenchymal CMs microtissues be fully dissected. This gap...
Abstract Technological advancements have enabled the design of increasingly complex engineered tissue constructs, which better mimic native cellularity. Therefore, dissecting bi-directional interactions between distinct cell types in 3D is necessary to understand how heterotypic at single-cell level impact tissue-level properties. We systematically interrogated cardiomyocytes (CMs) and cardiac non-myocytes self-assembled constructs an effort determine phenotypic functional contributions...
Engineered tissues are being used clinically for tissue repair and replacement, developed as tools drug screening human disease modeling. Self-assembled offer advantages over scaffold-based engineering, such enhanced matrix deposition, strength, function. However, there few available methods fabricating 3D without seeding cells on or within a supporting scaffold. Previously, we system self-assembled rings by into non-adhesive agarose wells. A polydimethylsiloxane (PDMS) negative was first...
This paper focuses on CMOS biosensor technologies for cellular biosensing applications. We first present our to achieve on-CMOS cell culture, maintenance, and differentiation, as the basis biosensors. Next, we introduce a frequency-shift magnetic sensor scheme which performs detections without post-processing or external biasing field. Finally, will demonstrate based achieves real-time chemical detections; such can be utilized massively paralleled high-throughput screening in drug development.
This work presents a disposable `electronic microplate' (e-microplate) platform that enables the reusability of corresponding CMOS biosensor thereby reducing cost and increasing throughput relative to non-disposable systems. The e-microplate utilizes mechanically-flexible interconnects (MFIs) through-silicon-vias (TSVs) electrically interconnect electrodes on while maintaining physical separation between aforementioned substrate tiers. Measurements performed show incorporation does not...
A silicon membrane acting as an interface layer between live cells and the sensing electronics enabling low-cost, high-throughput bio-sensing is proposed; capable of supporting high pixel density allowing accurate image mapping. Cell attachment growth was carried out on five different based surfaces compared to standard Tissue Culture Polystyrene (TCPS) surface in order optimize selection process material; cell imaging performed after 48 hours study effect variations (different materials...
We propose to perform a live demonstration on our recently developed multi-modality CMOS sensor array whose pixel each integrates several biosensors with different sensing modalities. Unlike many single-modality sensors, this can real-time capture multi-physics responses of living cells and tissues high spatiotemporal resolution. This will showcase the operation its detection human ovarian cancer HeyA8-F8 under chemical administrations.
Epicardial cells are a crucial component in constructing
Abstract Induced pluripotent stem cell derived cardiomyocytes (iPSC-CMs) are used to model cardiac development and disease. This requires a robust population of mature CMs external stimuli mimic the complex environment heart. In effort toward this maturation, previous groups have applied electrical stimulation (ES) with varying results depending on duration, frequency, pattern. As such, there is uncertainty surrounding timeline which stimulated iPSC-CMs begin show early signs maturation in...
Abstract Few clinical solutions exist for cardiac fibrosis, creating the need a tunable in vitro model to better understand fibrotic disease mechanisms and screen potential therapeutic compounds. Here, we combined cardiomyocytes, fibroblasts, exogenous extracellular matrix (ECM) proteins create an environmentally-mediated fibrosis model. Cells ECM were into 2 types of tissues-aggregates tissue rings. The addition collagen I had drastic negative impact on aggregate formation, but ring...