A5014135548- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Antimicrobial Peptides and Activities
- Mesenchymal stem cell research
- Antimicrobial agents and applications
- Angiogenesis and VEGF in Cancer
- Proteoglycans and glycosaminoglycans research
- Additive Manufacturing and 3D Printing Technologies
- Cardiac Structural Anomalies and Repair
- Cardiac and Coronary Surgery Techniques
- Protein Hydrolysis and Bioactive Peptides
- Immune Response and Inflammation
- Biomedical and Engineering Education
- Silk-based biomaterials and applications
- Coronary Interventions and Diagnostics
- Bone fractures and treatments
- Tracheal and airway disorders
- Innovative Microfluidic and Catalytic Techniques Innovation
- Lipid Membrane Structure and Behavior
- Nanotechnology research and applications
- Enzyme function and inhibition
- Aldose Reductase and Taurine
- Hydrogels: synthesis, properties, applications
Worcester Polytechnic Institute
2015-2024
Northeastern University
2023-2024
World Precision Instruments (United States)
2020
University of Tübingen
2019
Benaroya Research Institute
2006-2010
Virginia Mason Medical Center
2009
University of Washington
2007
Despite significant advances in the fabrication of bioengineered scaffolds for tissue engineering, delivery nutrients complex engineered human tissues remains a challenge. By taking advantage similarities vascular structure plant and animal tissues, we developed decellularized as prevascularized scaffold engineering applications. Perfusion-based decellularization was modified different species, providing geometries scaffolding. After decellularization, remained patent able to transport...
Large-scale musculoskeletal wounds, such as those seen in trauma injuries, present poor functional healing prognoses. In severe trauma, when the native tissue architecture is destroyed or lost, regenerative capacity of skeletal muscle diminished by scar formation. Here we demonstrate that a scaffold system composed fibrin microthreads can provide an efficient delivery for cell-based therapies and improve regeneration large defect tibialis anterior mouse. Cell-loaded microthread bundles...
There is an urgent need for efficient approach to obtain a large-scale and renewable source of functional human vascular smooth muscle cells (VSMCs) establish robust, patient-specific tissue model systems studying the pathogenesis disease, developing novel therapeutic interventions. Here, we have derived large quantity highly enriched VSMCs from induced pluripotent stem (hiPSC-VSMCs). Furthermore, engineered 3D rings hiPSC-VSMCs using facile one-step cellular self-assembly approach. The are...
The goal of this study was to develop a system rapidly generate engineered tissue constructs from aggregated cells and cell-derived extracellular matrix (ECM) enable evaluation structure function. Rat aortic smooth muscle seeded into annular agarose wells (2, 4 or 6 mm inside diameter) formed thick rings within 2 weeks static culture (0.76 at 8 days; 0.94 14 days). Overall, appeared healthy surrounded by ECM comprised glycosoaminoglycans collagen, although signs necrosis were observed near...
Abstract Currently, there are no synthetic or biologic materials suitable for long‐term treatment of large tracheal defects. A successful replacement must (1) have radial rigidity to prevent airway collapse during respiration, (2) contain an immunoprotective respiratory epithelium, and (3) integrate with the host vasculature support epithelium viability. Herein, biopolymer microspheres used deliver chondrogenic growth factors human mesenchymal stem cells (hMSCs) seeded in a custom mold that...
Smooth muscle cell (SMC) diversity and plasticity are limiting factors in their characterization application cardiovascular tissue engineering. This work aimed to evaluate the potential of Raman microspectroscopy imaging distinguish SMCs different origins phenotypes. Cultured human isolated from vascular non-vascular tissues as well fixed SMC-containing were analyzed. In addition, spectra images tissue-engineered SMC constructs acquired. Routine techniques such qPCR, histochemistry,...
Embryonic stem cells (ESCs) provide a convenient model to probe the molecular and cellular dynamics of developmental cell morphogenesis. ESC differentiation in vitro via embryoid bodies (EBs) recapitulates many aspects early stages development, including epithelial-mesenchymal transition (EMT) pluripotent into more differentiated progeny. Hyaluronan versican are important extracellular mediators EMT processes, yet temporal expression spatial distribution these matrix (ECM) molecules during...
Abstract We developed a method to produce discrete fibrin microthreads, which can be seeded with human mesenchymal stem cells (hMSCs) and used as suture enhance the efficiency localization of cell delivery. To assess efficacy microthreads support hMSC attachment, proliferation, survival, (100 μm diameter per microthread) were bundled together, 50,000 hMSCs for 2 h, cultured 5 days. Cell density on microthread bundles increased over time in culture maximum average 731 ± 101 cells/mm after A...
Abstract Advances in regenerative medicine have improved the potential of using cellular therapy for treating several diseases. However, effectiveness new therapies is largely limited by low cell engraftment and inadequate localization. To improve on these limitations, we developed a novel delivery mechanism cell‐seeded biological sutures. We demonstrate ability sutures to efficiently implant human mesenchymal stem cells (hMSCs) specific regions within beating heart; tissue known retention...
Abstract Sewing cuffs incorporated within tissue‐engineered blood vessels (TEBVs) enable graft anastomosis in vivo , and secure TEBVs to bioreactors vitro . Alternative approaches cuff design are required achieve integration with scaffold‐free during tissue maturation. To create porous materials that promote integration, we used electrospinning fabricate from polycaprolactone (PCL), PCL blended gelatin, coated evaluated mechanical properties, porosity, cellular attachment infiltration....
Abstract Proteoglycans and hyaluronan play critical roles in heart development. In this study, human embryonic stem cells (hESC) were used as a model to quantify the synthesis of proteoglycans hESC early stages differentiation, after directed differentiation into cardiomyocytes. We demonstrated that both cardiomyocyte cultures synthesize an extracellular matrix (ECM) enriched hyaluronan. During total proteoglycan decreased proportion bearing heparan sulfate chains was reduced. Versican,...
Each year, hundreds of thousands patients undergo coronary artery bypass surgery in the United States.1 Approximately one third these do not have suitable autologous donor vessels due to disease progression or previous harvest. The aim vascular tissue engineering is develop a alternative source for grafts. In addition, engineered may prove valuable as living models study cardiovascular diseases. Several promising approaches blood been explored, with many recent studies focusing on...
Abstract This study explores the bioprinting of a smooth muscle cell-only bioink into ionically crosslinked oxidized methacrylated alginate (OMA) microgel baths to create self-supporting vascular tissues. The impact OMA support bath methacrylation degree and dispensing parameters on tissue formation, remodeling, structure strength was investigated. We hypothesized that reducing tip diameter from 27 G (210 μ m) 30 (159 for would reduce wall thickness improve consistency dimensions while...
Abstract In this study, we created self‐assembled smooth muscle cell (SMC) tissue rings (comprised entirely of cells and cell‐derived matrix; CDM) compared their structure material properties with from SMC‐seeded fibrin or collagen gels. All were cultured statically for 7 days in supplemented growth medium (with ε‐amino caproic acid, ascorbic insulin‐transferrin‐selenium), prior to uniaxial tensile testing histology. Self‐assembled CDM exhibited ultimate strength stiffness values that...
Cellular self-assembly has been used to generate living tissue constructs as an alternative seeding cells on or within exogenous scaffold materials. However, high cell and extracellular matrix density in self-assembled may impede diffusion of growth factors during engineered culture. In the present study, we assessed feasibility incorporating gelatin microspheres vascular rings cellular achieve factor delivery. To assess microsphere incorporation distribution rings, were mixed with a...
Engineered tissues are being used clinically for tissue repair and replacement, developed as tools drug screening human disease modeling. Self-assembled offer advantages over scaffold-based engineering, such enhanced matrix deposition, strength, function. However, there few available methods fabricating 3D without seeding cells on or within a supporting scaffold. Previously, we system self-assembled rings by into non-adhesive agarose wells. A polydimethylsiloxane (PDMS) negative was first...
Tissue-engineered human blood vessels may enable in vitro disease modeling and drug screening to accelerate advances vascular medicine. Existing methods for tissue-engineered vessel (TEBV) fabrication create homogenous tubes not conducive the focal pathologies characteristic of certain diseases. We developed a system generating self-assembled smooth muscle cell (SMC) ring units, which were fused together into TEBVs. The goal this study was assess feasibility modular assembly fusion building...