A5101692446- Ion channel regulation and function
- Lipid Membrane Structure and Behavior
- Electron Spin Resonance Studies
- Force Microscopy Techniques and Applications
- Erythrocyte Function and Pathophysiology
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Blood Coagulation and Thrombosis Mechanisms
- Cardiac and Coronary Surgery Techniques
- Seismology and Earthquake Studies
- Circular RNAs in diseases
- COVID-19 diagnosis using AI
- Plant and Biological Electrophysiology Studies
- Analytical Chemistry and Sensors
- Venous Thromboembolism Diagnosis and Management
- Molecular Junctions and Nanostructures
- Mechanical and Optical Resonators
- Spectroscopy and Quantum Chemical Studies
- RNA modifications and cancer
- Nanopore and Nanochannel Transport Studies
- Ferroptosis and cancer prognosis
Baoding People's Hospital
2025
Affiliated Hospital of Chengde Medical College
2025
Manchester Academic Health Science Centre
2024
University of Manchester
2024
Beijing Anzhen Hospital
2023
Capital Medical University
2023
University of Leeds
2020-2022
Institute of Structural and Molecular Biology
2021
University of St Andrews
2019
Abstract Lipid availability within transmembrane nano-pockets of ion channels is linked with mechanosensation. However, the effect hindering lipid-chain penetration into on channel structure has not been demonstrated. Here we identify large conductance mechanosensitive MscL, high-pressure threshold channel. We restrict access to by mutagenesis and sulfhydryl modification, monitor conformation PELDOR/DEER spectroscopy. For a single site located at entrance distal pore generate an allosteric...
Membrane forces shift the equilibria of mechanosensitive channels enabling them to convert mechanical cues into electrical signals. Molecular tools stabilize and methods capture their highly dynamic states are lacking. Cyclodextrins can mimic tension through sequestering lipids from membranes. Here we probe conformational ensemble MscS by EPR spectroscopy, lipid environment with NMR, function electrophysiology under cyclodextrin-induced tension. We show extent activation depends on...
The mechanosensitive ion channel of large conductance MscL gates in response to membrane tension changes. Lipid removal from transmembrane pockets leads a concerted structural and functional response, but it remains unknown whether there is correlation between the tension-mediated state derived by pocket delipidation absence tension. Here, we combined pulsed electron paramagnetic resonance spectroscopy hydrogen-deuterium exchange mass spectrometry, coupled with molecular dynamics simulations...
This study aimed to investigate the correlation between M2 macrophages activity with prognosis of lung adenocarcinoma (LUAD). We sought identify key genes associated macrophage and examine their relationship clinicopathological features elucidate underlying mechanism. Published datases were analyzed for differentially expressed genes. After quality control, batch effect removal, annotation, scRNA dataset identified macrophage-associated in LUAD group, which cross-analyzed referred as...
Solvent accessibilities of and distances between protein residues measured by pulsed-EPR approaches provide high-resolution information on dynamic motions. We describe protocols for the purification site-directed spin labeling integral membrane proteins. In our protocol, peptide-level HDX-MS is used as a precursor to guide single-residue resolution ESEEM accessibility measurements strategies EPR applications. Exploiting pentameric MscL channel model, we discuss use cwEPR, DEER/PELDOR,...
Mechanosensitive ion channels are integral membrane proteins ubiquitously present in bacteria, archaea, and eukarya. They act as molecular sensors of mechanical stress to serve vital functions such touch, hearing, osmotic pressure, proprioception balance, while their malfunction is often associated with pathologies. Amongst them, the structurally distinct MscL MscS from bacteria most extensively studied. MscS-like have been found plants Schizosaccharomyces pombe, where they regulate...
Abstract The MscL channel gates in response to membrane tension changes allow the exchange of molecules through its pore. Lipid removal from transmembrane pockets leads a response. However, it is unknown whether there correlation between mediated state and derived by pocket delipidation absence tension. Transitions states may follow similar pathway cover available conformational space but not necessarily sample same discrete intermediates. Here, we combined pulsed-EPR HDX-MS measurements on...
To verify the administration of a new nano delivery system coated with Tirofiban on preventing early thrombosis in vein graft.