A5017436608- Advanced biosensing and bioanalysis techniques
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Nanopore and Nanochannel Transport Studies
- Ethics and bioethics in healthcare
- Anodic Oxide Films and Nanostructures
- Gold and Silver Nanoparticles Synthesis and Applications
Chongqing Medical and Pharmaceutical College
2020-2024
Southwest Hospital
2020
117th Hospital of People's Liberation Army
2020
Army Medical University
2020
Accurate identification of exosomes plays an essential role in facilitating disease diagnosis and therapies. Herein, we proposed Aptamer-cholesterol-mediated Proximity Ligation Assay (AcmPLA) for accurate a dual-probe strategy, one aptamer probe recognition exosomal innate surface protein CD63 another cholesterol biolipid layer targeting. By integrating proximity ligation probes bound with biomarkers specific rolling circle amplification (RCA) strategy signal amplification, have successfully...
Extracellular vesicles (EVs) are emerging as promising biomarkers for cancer diagnosis and therapy. Recognizing low-abundance EVs from clinical samples in an easy-to-operate way is highly desired but remains a challenge. Herein, we established allosteric probe-initiated dual cycle amplification-assisted CRISPR-Cas12a (AID-Cas) platform sensitive detection of wash-free way. In AID-Cas, the probe can specifically recognize bind with target thus initiate following dual-cycle amplification....
Signal amplification strategies have emerged as a prominent tool in the field of improving detection sensitivity small extracellular vesicles (sEVs). It is important to highlight that utilization signal quenching not commonly implemented. A technique for sEVs was established based on unwinding G-quadruplex using Klenow fragment polymerase (KF), which served an inspiration this study. This system characterized by its simplicity and lack labeling, making it efficient approach quenching. In...