A5102910193- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Healthcare and Environmental Waste Management
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Peptidase Inhibition and Analysis
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Chronic Obstructive Pulmonary Disease (COPD) Research
- T-cell and B-cell Immunology
- Ethics in Clinical Research
- Biomedical and Engineering Education
- Infection Control in Healthcare
- Quality and Safety in Healthcare
- Pulmonary Hypertension Research and Treatments
- Aging, Health, and Disability
- Protein Degradation and Inhibitors
- Medical Imaging and Pathology Studies
- Data Quality and Management
- Neonatal Respiratory Health Research
- CAR-T cell therapy research
- Semantic Web and Ontologies
- Big Data and Business Intelligence
The Ohio State University
2013-2025
University of Pennsylvania
2020-2025
Universidad Politécnica de Madrid
2017-2023
Metropolitan University of Technology
2021
Histone acetyltransferase 1 is an evolutionarily conserved type B histone that thought to be responsible for the diacetylation of newly synthesized H4 on lysines 5 and 12 during chromatin assembly. To understand function this enzyme in a complex organism, we have constructed conditional mouse knockout model Hat1. Murine Hat1 essential viability, as homozygous deletion results neonatal lethality. The lungs embryos pups genetically deficient were much less mature upon histological evaluation....
Histone acetyltransferase 1 (Hat1) catalyzes the acetylation of newly synthesized histone H4 at lysines 5 and 12 that accompanies replication-coupled chromatin assembly. The occurs in cytoplasm function this is typically ascribed to roles either nuclear import or deposition. Using cell lines from Hat1+/+ Hat1−/− mouse embryos, we demonstrate Hat1 not required for We employed quantitative proteomics characterize Hat1-dependent changes composition nascent structure. Among proteins depleted...
Post-translational modifications of histones are significant regulators replication, transcription, and DNA repair. Particularly, newly synthesized histone H4 in H3/H4 heterodimers becomes acetylated on N-terminal lysine residues prior to its incorporation into chromatin. Previous studies have established that the acetyltransferase (HAT) complex Hat1p/Hat2p medicates this modification. However, mechanism how recognizes facilitates enzymatic activities assembled heterodimer remains unknown....
Abstract Histone acetyltransferase 1 (Hat1) is responsible for the acetylation of newly synthesized histone H4 on lysines 5 and 12 during process chromatin assembly. To understand broader biological role Hat1, we have generated a conditional mouse knockout model this enzyme. We previously reported that Hat1 required viability important mammalian development genome stability. In study, show haploinsufficiency results in significant decrease lifespan. Defects observed +/− mice are consistent...
The replisome is a protein complex on the DNA replication fork and functions in dynamic environment at intersection of parental nascent chromatin. Parental nucleosomes are disrupted front fork. daughter duplexes packaged with an equal amount newly synthesized histones wake through activity replication-coupled chromatin assembly pathway. Histone acetyltransferase 1 (HAT1) responsible for cytosolic diacetylation histone H4 lysines 5 12, which accompanies assembly. Here, using proximity...
Lysine acetylation has emerged as one of the most important post-translational modifications, regulating different biological processes. However, its regulation by lysine acetyltransferases is still unclear in cases. Hat1 a acetyltransferase originally identified based on ability to acetylate histones. Using an unbiased proteomics approach, we have determined how loss affects mammalian acetylome. Hat1+/+ and Hat1-/- mouse embryonic fibroblast cell lines were grown both glucose-...
Nowadays, companies and official bodies are using the data as a principal asset to take strategic decisions.The advances in big processing, storage analysis techniques have allowed manage continuous increase volume of data.This together with its high variability large number sources lead constant growing complexity management environment.Data governance is key for simplifying that complexity: it element controls decision making responsibilities all processes related management.This paper...
ABSTRACT Exhausted CD8 T cells (T EX ) arising during chronic infections and cancer have reduced functional capacity limited fate flexibility that prevents optimal disease control response to immunotherapies. Compared memory MEM cells, a unique open chromatin landscape underlying distinct gene expression program. How transcriptional epigenetic landscapes are regulated through histone post-translational modifications (hPTMs) remains unclear. Here, we profiled key activating (H3K27ac H3K4me3)...
La inclusión del cobre como material antibacteriano en la arquitectura sanitaria ayuda a resolver gran paradoja que existe los servicios asistenciales; entrar de salud para sanarse una enfermedad puntual y adquirir enfermedades riesgo muerte. Esta investigación demuestra eficacia formato laminar vez sólido, haciendo mediciones con luminometría, abriendo un camino factible el revestimiento dotando su propiedad antibacteriana superficial costos reducidos, sin necesidad cambios mobiliario ni...
ABSTRACT Lysine acetylation has emerged as one of the most important post-translational modifications, regulating different biological processes. However, its regulation by lysine acetyltransferases is still unclear in cases. Hat1 a acetyltransferase originally identified based on ability to acetylate histones. Using an unbiased proteomics approach, we have determined how loss affects mammalian acetylome. +/+ and −/− mouse embryonic fibroblast (MEF) cells lines were grown both glucose-...
ABSTRACT The replisome functions in a dynamic environment that is at the intersection of parental and nascent chromatin. Parental nucleosomes are disrupted front replication fork. daughter duplexes packaged with an equal amount newly synthesized histones wake fork through action replication-coupled chromatin assembly pathway. Histone acetyltransferase 1 (Hat1) responsible for cytosolic diacetylation histone H4 on lysines 5 12 accompanies assembly. Analysis role Hat1 demonstrates also...