A5079036030- Collagen: Extraction and Characterization
- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Connective tissue disorders research
- Periodontal Regeneration and Treatments
- Peptidase Inhibition and Analysis
- Enzyme Production and Characterization
- Bone and Dental Protein Studies
- Protein Hydrolysis and Bioactive Peptides
- Autophagy in Disease and Therapy
- Bone Tissue Engineering Materials
- Cellular Mechanics and Interactions
- Silymarin and Mushroom Poisoning
- Hippo pathway signaling and YAP/TAZ
- Pancreatic function and diabetes
- Prion Diseases and Protein Misfolding
- Silk-based biomaterials and applications
- Skin and Cellular Biology Research
- interferon and immune responses
- Monoclonal and Polyclonal Antibodies Research
- Proteoglycans and glycosaminoglycans research
- Endoplasmic Reticulum Stress and Disease
- Skin Protection and Aging
- Wnt/β-catenin signaling in development and cancer
- NF-κB Signaling Pathways
Japan Institute of Leather Research
2016-2025
Jikei University School of Medicine
2023
Kyoto University
2015
Hamamatsu University School of Medicine
2012
National Institute of Infectious Diseases
1998-2011
The University of Tokyo
2000
National Institutes of Health
1999
Tokyo Medical and Dental University
1987-1996
University of Illinois Urbana-Champaign
1987
To evaluate the function of cultured human corneal endothelial cells (HCECs) in vivo and feasibility HCEC transplantation with a collagen sheet as substitute carrier HCECs.Adult donor cornea derived from HCECs was labeled fluorescent tracker DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate) seeded on sheet. The pump evaluated by measurement potential difference short-circuit current. A 6-mm sclerocorneal incision Descemetorhexis were performed rabbit eyes. brought into...
Although the extracellular matrix (ECM) plays essential roles in heart tissue engineering, optimal ECM components for organization have not previously been elucidated. Here, we focused on main component, fibrillar collagen, and analyzed effects of collagens by comparing use porcine heart-derived collagen other organ-derived generating engineered (EHT). We demonstrate that induces better contraction relaxation human induced pluripotent stem cell-derived EHT (hiPSC-EHT) hiPSC-EHT with exhibit...
Abstract Despite the success to prevent or limit cardiovascular diseases, restoration of function a damaged heart remains formidable challenge. Cardiac stem cells (CSCs), with capacity differentiate into cardiomyocytes, hold great potential as source for regenerative medicine. A major challenge facing clinical application differentiated CSCs, however, is theability generate sufficient numbers desired phenotype. We previously established cell lines CSCs using c‐kit antibody from adult rat...
Human neutrophil elastase was purified to homogeneity as two isozymes named E1 and E2. The degraded Type IV collagen, laminin, fibronectin, heparan sulfate proteoglycan similarly each other. degradation of such basement membrane components by may assist the extravasation neutrophils in process inflammation. Among substrates tested, only type V which is susceptible gelatinase, resistant elastase. This broad substrate specificity enzyme also contribute tissue destruction at sites We produced a...
Collagen-derived hydroxyproline (Hyp)-containing dipeptides and tripeptides, which are known to possess physiological functions, appear in blood at high concentrations after oral ingestion of gelatin hydrolysate. However, highly accurate sensitive quantification the Hyp-containing peptides has been challenging because analytical interference from numerous other components. We recently developed a stable isotope-labeled collagen named "SI-collagen" that can be used as an internal standard...
Recent studies have reported that oral intake of gelatin hydrolysate has various beneficial effects, such as reduction joint pain and lowering blood sugar levels. In this study, we produced a novel using cysteine-type ginger protease having unique substrate specificity with preferential peptide cleavage Pro at the P2 position. Substantial amounts X-hydroxyproline (Hyp)-Gly-type tripeptides were generated up to 2.5% (w/w) concomitantly Gly-Pro-Y-type (5%; w/w) powder. The in vivo absorption...
Skeletal muscle regeneration is a complicated process, requiring the proliferation, migration and differentiation of myoblasts whose processes are highly regulated by extracellular matrix (ECM) surrounding tissues in vivo. However, effects respective ECM components on regulation myoblast behaviors unknown. In this study, we report effect collagen I, major component tissue popular food supplement, mouse C2C12 as well underlying mechanisms. Collagen I (col 1) enhances myogenic cells, but has...
Alkaline treatment is a good method for extracting collagen with high recovery even from an aged animal specimen. However, the properties of treated under alkaline conditions have not been well established yet. By solution 3% sodium hydroxide and 1.9% monomethylamine, isoelectric point type I was lowered 9.3 to 4.8 because conversions Asn Gln Asp Glu. With acidification pI, denaturation temperature decreased 42 35 degrees C after 20 d treatment, but collagen-specific triple helical...
Activin A, a member of the transforming growth factor-β family, plays important roles in hormonal homeostasis and embryogenesis. In this study, we produced recombinant human activin A examined its abilities to bind extracellular matrix proteins. Recombinant expressed 293-F cells was purified as complexes mature dimeric with pro-region. Among panel proteins tested, bound perlecan agrin, but not laminins, nidogens, collagens I IV, fibronectin, nephronectin. The binding inhibited by heparin...
In recent years, glycopeptide purification by hydrazide chemistry has become popular in structural studies of glycoconjugates; however, applications this method have been almost completely restricted to analysis the N-glycoproteome. Here we report a novel for analyzing O-glycosylations unique collagen, which are attached hydroxylysine and include galactosyl-hydroxylysine glucosyl-galactosyl-hydroxylysine. We established chemistry-based using (1) galactose oxidase introduce an aldehyde into...
Collagens are the most abundant proteins in animals and involved many physiological/pathological events. Although various methods have been used to quantify collagen its post-translational modifications (PTMs) over years, it is still difficult accurately type-specific minor PTMs. We report a novel quantitative method targeting using stable isotope-labeled named "SI-collagen", which was labeled with isotopically heavy lysine, arginine, proline fibroblasts culture. prepared highly purified...