A5056489825- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Congenital heart defects research
- Heart Failure Treatment and Management
- Electrospun Nanofibers in Biomedical Applications
- Pulmonary Hypertension Research and Treatments
- Neuroscience and Neural Engineering
- Cardiovascular Function and Risk Factors
- CAR-T cell therapy research
- Cardiac Ischemia and Reperfusion
- Palliative Care and End-of-Life Issues
- Blood Coagulation and Thrombosis Mechanisms
- Complement system in diseases
- Cardiac Imaging and Diagnostics
- ATP Synthase and ATPases Research
- Transplantation: Methods and Outcomes
- Cardiac Structural Anomalies and Repair
- Renal and related cancers
- Coronary Interventions and Diagnostics
- Medication Adherence and Compliance
- Acute Myocardial Infarction Research
- 3D Printing in Biomedical Research
- Virus-based gene therapy research
- Mechanical Circulatory Support Devices
Keio University
2014-2024
Keio University Hospital
2015-2024
Japan Pediatric Society
2023
Yoshino Kogyosho (Japan)
2018-2019
StemCells (United States)
2019
Sophia University
1974
The clinical application of human induced pluripotent stem cell-derived cardiomyocytes (CMs) for cardiac repair commenced with the epicardial delivery engineered tissue; however, feasibility direct CMs into muscle layer, which has reportedly electrical integration, is unclear because concerns about poor engraftment and posttransplant arrhythmias. Thus, in this study, we prepared purified spheroids (hiPSC-CSs) investigated whether their injection could regenerate infarcted nonhuman primate hearts.
Cardiac regenerative therapies utilizing human induced pluripotent stem cells (hiPSCs) are hampered by ineffective large-scale culture. hiPSCs were cultured in multilayer culture plates (CPs) with active gas ventilation (AGV), resulting stable proliferation and pluripotency. Seeding of 1 × 106 per layer yielded 7.2 108 4-layer CPs 1.7 109 10-layer sequentially differentiated into cardiomyocytes (CMs) a two-dimensional (2D) differentiation protocol. The efficiency cardiac using AGV was...
Deep learning technology is rapidly advancing and now used to solve complex problems. Here, we deep in convolutional neural networks establish an automated method identify endothelial cells derived from induced pluripotent stem (iPSCs), without the need for immunostaining or lineage tracing. Networks were trained predict whether phase-contrast images contain based on morphology only. Predictions validated by comparison immunofluorescence staining CD31, a marker of cells. Method parameters...
The severe shortage of donor hearts hampered the cardiac transplantation to patients with advanced heart failure. Therefore, regenerative therapies are eagerly awaited as a substitution. Human induced pluripotent stem cells (hiPSCs) realistic cell source for cardiomyocytes. hiPSC-derived cardiomyocytes highly expected help recovery heart. Avoidance teratoma formation and large-scale culture definitely necessary clinical setting. combination pure spheroids gelatin hydrogel succeeded recover...
Although the extracellular matrix (ECM) plays essential roles in heart tissue engineering, optimal ECM components for organization have not previously been elucidated. Here, we focused on main component, fibrillar collagen, and analyzed effects of collagens by comparing use porcine heart-derived collagen other organ-derived generating engineered (EHT). We demonstrate that induces better contraction relaxation human induced pluripotent stem cell-derived EHT (hiPSC-EHT) hiPSC-EHT with exhibit...
Abstract Cardiac regenerative therapy with human pluripotent stem cells (hPSCs), such as embryonic and induced cells, has been hampered by the lack of efficient strategies for expanding functional cardiomyocytes (CMs) to clinically relevant numbers. The development massive suspension culture system (MSCS) shed light on this critical issue, although it remains unclear how hPSCs could differentiate into CMs using a MSCS. proliferative rate differentiating in MSCS was equivalent that cultures...
The role of lipid metabolism in human pluripotent stem cells (hPSCs) is poorly understood. We have used large-scale targeted proteomics to demonstrate that undifferentiated hPSCs express different fatty acid (FA) biosynthesis-related enzymes, including ATP citrate lyase and FA synthase (FASN), than those expressed hPSC-derived cardiomyocytes (hPSC-CMs). Detailed profiling revealed inhibition FASN resulted significant reduction sphingolipids phosphatidylcholine (PC); moreover, we found PC was...
Cell transplantation therapy will mean a breakthrough in resolving the donor shortage cardiac transplantation. Cardiomyocyte (CM) transplantation, however, has been relatively inefficient restoring function after myocardial infarction (MI) due to low engraftment of transplanted CM. In order ameliorate CM, novel strategy must be invented. Gelatin hydrogel (GH) is biodegradable water-soluble polymer gel. made collagen. Although we observed that collagen strongly induced aggregation platelets...
Cardiac regenerative therapy using human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) is expected to become an alternative heart transplantation for severe failure. It now possible produce large numbers of cells (hPSCs) and eliminate non-cardiomyocytes, including residual undifferentiated hPSCs, which can cause teratoma formation after transplantation. There are two main strategies transplanting hPSC-CMs: injection hPSC-CMs into the myocardium from epicardial side, implantation...
Human pluripotent stem cells (hPSCs) have a unique metabolic signature for maintenance of pluripotency, self-renewal, and survival. Although hPSCs could be potentially used in regenerative medicine, the prohibitive cost associated with large-scale cell culture presents major barrier to clinical application hPSC. Moreover, without fully characterized signature, hPSC conditions are not optimized. Here, we performed detailed amino acid profiling found that tryptophan (TRP) plays key role...
Failure of the right ventricle plays a critical role in any type heart failure. However, mechanism remains unclear, and there is no specific therapy. Here, we show that predominantly expresses alternative complement pathway-related genes, including Cfd C3aR1. Complement 3 (C3)-knockout attenuates ventricular dysfunction fibrosis mouse model C3a produced from C3 by convertase complex, which includes essential component factor D (Cfd). Cfd-knockout mice also attenuation Moreover, plasma...
Induced pluripotent stem cells (iPSCs) have been proposed as novel cell sources for genetic disease models and revolutionary clinical therapies. Accordingly, human iPSC-derived cardiomyocytes are potential cardiomyocyte transplantation therapy. We previously developed a generation method peripheral T cell-derived iPSCs (TiPSCs) that uses minimally invasive approach to obtain patient cells. However, it remained unknown whether TiPSCs with genomic rearrangements in the receptor (TCR) gene...
Recently, induced pluripotent stem cells (iPSCs) were established as promising cell sources for revolutionary regenerative therapies. The initial culture system used iPSC generation needed fetal calf serum in the medium and mouse embryonic fibroblast a feeder layer, both of which could possibly transfer unknown exogenous antigens pathogens into population. Therefore, development systems designed to minimize such potential risks has become increasingly vital future applications iPSCs clinical...
Pulmonary artery pressure (PAP)-guided therapy in patients with heart failure (HF) using the CardioMEMS (CMM) device, an implantable PAP sensor, has been shown to reduce HF hospitalizations previous studies. We sought evaluate clinical benefit of CMM device regard 30, 90, and 180 day readmission rates real-world usage.