A5040884081- Cardiac Arrhythmias and Treatments
- Virus-based gene therapy research
- Complement system in diseases
- Corneal surgery and disorders
- RNA Interference and Gene Delivery
- Cardiac electrophysiology and arrhythmias
- Cardiac pacing and defibrillation studies
- Cardiomyopathy and Myosin Studies
- Congenital Heart Disease Studies
- Blood groups and transfusion
- Renal Diseases and Glomerulopathies
- Glaucoma and retinal disorders
- Ophthalmology and Visual Impairment Studies
- Liver physiology and pathology
- Congenital heart defects research
- CAR-T cell therapy research
- Intraocular Surgery and Lenses
- Ocular Surface and Contact Lens
- Atrial Fibrillation Management and Outcomes
- Cell death mechanisms and regulation
- Ion channel regulation and function
- Viral Infectious Diseases and Gene Expression in Insects
- Immunotherapy and Immune Responses
- Cardiovascular Effects of Exercise
- Cardiac Valve Diseases and Treatments
Osaka City General Hospital
2015-2025
Minamiaoyama Eye Clinic
2008-2024
Kyoto University
1998-2024
The University of Tokyo
1966-2023
Kajima Corporation (Japan)
2021-2023
YKK (Japan)
2023
Japanese Foundation For Cancer Research
1994-2022
D. Western Therapeutics Institute (Japan)
2012-2022
Mie University
2021-2022
The Nakamura Hajime Eastern Institute
2021
Thrombotic thrombocytopenic purpura (TTP) is a type of thrombotic microangiopathy (TMA). Studies report that the majority TTP patients present with deficiency ADAMTS13 activity. In database TMA in Japan identified between 1998 and 2008, 186 first onset acquired idiopathic (ai) ADAMTS13-deficient (ADAMTS13 activity <5%) were diagnosed. The median age this group was 54 years, 54.8% female, 75.8% had renal involvement, 79.0% neurologic symptoms, 97.8% detectable inhibitors to Younger less...
We have developed a recombinant replication–defective adenovirus containing human α–fetoprotein (AFP) promoter/enhancer to direct cell type–specific expression of the herpes simplex virus thymidine kinase (HSV tk ) gene AFP–producing hepatocellular carcinoma (HCC) cells. After an in vitro infection by carrying lacZ under control AFP (AdAFP ), was demonstrated efficiently HuH–7 and HepG2 lines, but not AFP–nonproducing HLE HLF although all these lines when infected with vector driven...
Recombinant adenovirus (Adv)-mediated gene transduction is a powerful technology for cancer therapy. In this article, we report the generation of fiber-mutant Adv vector, using genomic DNA–terminal protein complex (DNA–TPC) cotransfection method. First, construct in plasmid carrying right-side two-thirds human type 5 (Ad5) genome (pTR) was cotransfected with Ad5 DNA–TPC, yielding recombinant desired fiber mutation. The DNA–TPC from mutant then utilized to produce second-step an expression...
To evaluate the changes in vault over time after Implantable Collamer Lens (ICL; STAAR Surgical) posterior chamber phakic intraocular lens implantation.A total of 36 eyes 19 patients who underwent ICL implantation were enrolled study. Vault (the distance between back surface and front crystalline lens) was measured postoperatively using anterior optical coherence tomography. Patients divided into three groups based on (low < or =0.25 mm, moderate >0.25 to <0.75 high > =0.75 mm).Mean at 1...
Atypical hemolytic uremic syndrome (aHUS) is caused by complement overactivation, and its presentation prognosis differ according to the underlying molecular defects. The aim of this study was characterize genetic backgrounds aHUS patients in Japan elucidate associations between their backgrounds, clinical findings, outcomes.We conducted a nationwide epidemiological survey clinically diagnosed examined 118 enrolled from 1998 2016 Japan. We screened variants seven genes related coagulation,...
For thrombotic microangiopathies (TMAs), the diagnosis of atypical hemolytic uremic syndrome (aHUS) is made by ruling out Shiga toxin-producing Escherichia coli (STEC)-associated HUS and ADAMTS13 activity-deficient thrombocytopenic purpura (TTP), often using exclusion criteria for secondary TMAs. Nowadays, assays activity evaluation STEC infection can be performed within a few hours. However, confident aHUS requires comprehensive gene analysis alternative complement activation pathway, which...
Abstract We have established a panel of 45 human cancer cell lines (JFCR-45) to explore genes that determine the chemosensitivity these anticancer drugs. JFCR-45 comprises derived from tumors three different organs: breast, liver, and stomach. The inclusion gastric hepatic cancers is major point novelty this study. determined concentration 53 drugs could induce 50% growth inhibition (GI50) in each line. Cluster analysis using GI50s indicated allow classification based on their modes action,...
Astrocytic tumors frequently express Fas/APO-1 (Fas), in sharp contrast to surrounding normal brain cells, providing a potential window through which selective killing of tumor cells could be pursued. To assess this possibility, we transduced Fas into U251, glioma cell line resistant anti-Fas antibody-mediated apoptosis, and obtained transfectants with high levels expression. Anti-Fas antibody showed significantly enhanced cytotoxicity for the transfectants, suggesting that U251 maintained...
Abstract Background Developmental angiogenesis proceeds through multiple morphogenetic events including sprouting, intussusception, and pruning. Mice lacking the membrane-anchored metalloproteinase regulator Reck die in utero around embryonic day 10.5 with halted vascular development; however, mechanisms by which this phenotype arises remain unclear. Results We found that is abundantly expressed cells associated blood vessels undergoing or remodelling uteri of pregnant female mice. Some...
We investigated the change in urine albumin-to-creatinine ratio (ACR) to examine effect of sitagliptin on diabetic nephropathy.Sitagliptin at a dose 50 mg was administered 247 outpatients with type 2 diabetes. Data were collected patients' laboratory results (including ACR), blood pressure, and body weight. Clinical data compared before after 3 months' administration sitagliptin.The ACR changed from 150.0 ± 538.6 mg/gCre 148.3 764.6 over months. In patients micro- macro-albuminuria, months...