A5066955200- Allergic Rhinitis and Sensitization
- Asthma and respiratory diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Food Allergy and Anaphylaxis Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Contact Dermatitis and Allergies
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Dermatology and Skin Diseases
- MicroRNA in disease regulation
- Protein purification and stability
- Gold and Silver Nanoparticles Synthesis and Applications
- Pluripotent Stem Cells Research
- Immune Response and Inflammation
- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nitric Oxide and Endothelin Effects
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Urticaria and Related Conditions
- Cancer Immunotherapy and Biomarkers
- Protist diversity and phylogeny
- Advanced biosensing and bioanalysis techniques
Burgas Free University
2025
BOKU University
2015-2024
Institute of Molecular Biotechnology
2022-2024
Medical University of Vienna
2017-2023
John Wiley & Sons (United States)
2015
Bioengineering Center
2015
National Center for Genetic Engineering and Biotechnology
2015
Ragweed pollen represents a major allergy risk factor. extracts contain five different isoforms of the allergen Amb 1. However, immunological characteristics 1 are not fully investigated. Here, we compared physicochemical and properties three most important isoforms.After purification, were physicochemically characterized, tested for antibody binding induction human T-cell proliferative responses. Their further evaluated in vitro vivo mouse model.Amb exhibited distinct patterns IgE...
(1) Recombinant protein production in mammalian cells is either based on transient transfection processes, often inefficient and underlying high batch-to-batch variability, or laborious generation of stable cell lines. Alternatively, BacMam, a transduction process using the baculovirus, can be employed. (2) Six transfecting agents were compared to baculovirus terms expression characteristics model ACE2-eGFP HEK293-6E, CHO-K1, Vero Furthermore, optimization such as enhancement sodium butyrate...
Costimulatory signals potently promote T-cell proliferation and effector function. Agonistic antibodies targeting costimulatory receptors of the TNFR family, such as 4-1BB CD27, have entered clinical trials in cancer patients. Currently there is limited information how regulate antigen-specific but also bystander activation human CD8 T cells. Engineered antigen presenting cells (eAPC) efficiently several common viral epitopes on HLA-A2 combination with MHC class I tetramer staining were used...
MicroRNAs are small non-coding RNAs that play a critical role in post-transcriptional control of gene expression. Recent publications genomic sequencing data from the Chinese Hamster (CGR) and hamster ovary (CHO) cells provide new tools for discovery novel miRNAs this important production system. Version 20 miRNA registry miRBase contains 307 mature 200 precursor sequences CGR/CHO. We searched evolutionary conserved v20 recently published data, derived CHO cells, to further extend list known...
Aluminium salts have been used in vaccines for decades. However, the mechanisms underlying their adjuvant effect are still unclear. Neutrophils, first immune cells at injection site, can release cellular DNA together with granular material, so-called neutrophil extracellular traps (NETs). In mice, NETs apparently play a role aluminium hydroxide (alum)-adjuvant response to vaccines. Although no experimental data exist, this is assumed be operative also humans. As step verify knowledge humans,...
Aluminum hydroxide (alum) and monophosphoryl-lipid A (MPLA) are conventional adjuvants in vaccines for allergen-specific immunotherapy (AIT). Alum triggers the release of neutrophil extracellular traps (NETs) by neutrophils. NETs contain expelled decondensed chromatin associated with granular material may act as danger-associated molecular patterns activate antigen-presenting cells. We investigated whether adjuvant-induced contribute to innate responses AIT-vaccines. Human neutrophils were...
Abstract The antibacterial properties of cicada wings originate from hexagonally arranged pillar-like multi-functional nanostructures with species-dependent heights, which are super-hydrophobic and self-cleaning. In the present study, two species promising nanopillars were investigated in more detail. Selected methods used to analyze wing surfaces, including Atomic Force Microscopy, Scanning Electron bacterial tests live/dead staining. Verifying posed challenges, such as bacteria...
Skin resident T cells provide immediate immunologic responses at their specific location and play a role in the pathogenesis of skin diseases such as psoriasis. Recently, IL-9-producing were described major T-cell subtype present skin, but knowledge on biology situ regulation this is scarce. Here, we investigated cytokine influence with focus IL-9–producing because better understanding may identify novel therapeutic approaches. Healthy human biopsies cultured either presence IL-2, IL-4,...
Nature offers a wide range of evolutionary optimized materials that combine unique properties with intrinsic biocompatibility and can be exploited as biomimetic materials. The R5 RRIL peptides employed here are derived from silaffin proteins play crucial role in the biomineralization marine diatom silica shells also able to form vitro. Here, we demonstrate application particles vaccine delivery adjuvant platform by linking precipitating motif variety peptide antigens. resulting...
Allergen-specific immunotherapy (AIT) is the only treatment for type I allergy, which achieves long-lasting effects. Repeated subcutaneous applications of allergen extracts cause a protective antibody response and an immune deviation T cells. In AIT with allergoids, chemically modified are injected. During so-called special pre-seasonal application scheme, after initial phase applying increased doses allergoids followed by natural exposure as maintenance phase. The effectiveness allergoid...
Allergen-specific immunotherapy (AIT) is the only treatment for type I allergy, which achieves long-lasting effects. Repeated subcutaneous applications of allergen extracts cause a protective antibody response and an immune deviation T cells. In AIT with allergoids, chemically modified are injected. During so-called special pre-seasonal application scheme, after initial phase applying increased doses allergoids followed by natural exposure as maintenance phase. The effectiveness allergoid...