This study investigated whether the activities of antioxidant components donkey seminal plasma (SP)-both enzymatic (superoxide dismutase (SOD), catalase-like (CAT), glutathione peroxidase-like (GPX), and paraoxonase type 1 (PON1)) non-enzymatic (measured in terms total thiol, copper-reducing capacity (CUPRAC), ferric-reducing ability (FRAP), Trolox equivalent (TEAC))-and oxidative stress index (OSI) are related to sperm cryotolerance. For this purpose, 15 ejaculates from jackasses (one per...

Abstract Background Measurement of adenosine deaminase (ADA) can provide information about cell-mediated immunity. This report’s objective was to study the enzymatic activity total ADA (tADA) and its isoenzymes ADA1 ADA2 in canine, equine, porcine, bovine serum saliva their changes different inflammatory situations each species. Besides, an automated method for measurement developed validated. Results tADA present healthy animals four Erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA)...

The objective of this study was to determine the relationship enzymatic (superoxide dismutase, SOD; glutathione peroxidase, GPX; catalase, CAT; and paraoxonase type 1, PON1) non-enzymatic antioxidants (measured in terms of: Trolox equivalent antioxidant capacity, TEAC; cupric-reducing CUPRAC; ferric-reducing ability plasma, FRAP), as well oxidative stress index (OSI) seminal plasma (SP) with resilience horse sperm freeze-thawing. Twenty-one ejaculates (one per individual) were collected...

Hypothyroidism is the most common endocrine disorder diagnosed in dogs, leading to deleterious effects on a dog's life quality. This study aims evaluate changes redox status canine hypothyroidism. For this purpose, comprehensive panel of antioxidants and oxidants biomarkers were measured serum saliva 23 dogs with hypothyroidism, 21 non-thyroidal illness, 16 healthy dogs. Among antioxidants, cupric reducing antioxidant capacity (CUPRAC), ferric ability plasma (FRAP), Trolox equivalent (TEAC),...

The contribution of redox imbalance to equine asthma (EA) pathogenesis remains unclear. (1) validate and measure a panel biomarkers in the tracheal wash (TW) bronchoalveolar lavage (BAL) samples from horses with neutrophilic mastocytic mild-moderate EA (MEA) severe EA. (2) Evaluate same saliva serum for comparative purposes. A total 117 horses: 37 healthy, 26 MEA, 29 25 Cross-sectional study using TW, BAL, sampling. After assay validation, biomarkers-ferric reducing antioxidant power (FRAP),...

Changes in the oxidative status of blood horses suffering from gastric ulcers and colic intestinal aetiology (CIE) have been reported. However, saliva can also be a source biomarkers status. Therefore, this study aims to validate automated assays for measurement (ferric reducing ability saliva/serum-FRAS/FRAP, cupric antioxidant capacity-CUPRAC, Trolox equivalent capacity-TEAC, uric acid, advanced oxidation protein products-AOPP) serum horses, assess their changes different ulcer diseases...

The evaluation of the biomarkers oxidative status is usually performed in serum, however, other samples, such as red blood cells (RBCs) lysates or whole (WB), can be used. objective this study was to evaluate if a comprehensive panel redox measured WB and RBCs dogs, their possible changes "in vitro" after addition different concentrations ascorbic acid. integrated by antioxidant status, cupric reducing capacity (CUPRAC), ferric ability plasma (FRAP), Trolox equivalent (TEAC), thiol...

Saliva from pigs is gaining attention as an easy sample to obtain, being a source of biomarkers that can provide information on animal health and welfare. This study aimed evaluate the changes occur in salivary redox status with experimentally induced sepsis. For that, cupric reducing antioxidant capacity (CUPRAC), ferric ability saliva (FRAS), Trolox equivalent (TEAC), advanced oxidation protein products (AOPP), ferrous oxidation-xylenol orange (FOX), peroxide activity (POX-Act), reactive...

Hypothyroidism is the most commonly diagnosed endocrine disease in dogs. The objective of this study was to evaluate changes redox status canine hypothyroidism using whole blood (WB) and red cell (RBCs) lysates. For purpose, a panel five antioxidants oxidants biomarkers measured WB RBCs lysates 30 dogs with hypothyroidism, 26 non-thyroidal illnesses 15 healthy were cupric reducing antioxidant capacity (CUPRAC), ferric ability plasma (FRAP), Trolox equivalent (TEAC), thiol paraoxonase type-1...

Hypothyroidism is the most commonly diagnosed endocrine disorder in dogs. It produces a deficiency of thyroid hormones which impacts negatively dog's quality life. The objective this study to evaluate possible changes salivary metabolic profile dogs with hypothyroidism. For purpose, targeted metabolomics analysis performed by LC/MS was made saliva samples from group hypothyroidism and healthy Twenty-three metabolites showed significant decrease between hypothyroid dogs, these associated...

In this study, changes in salivary and serum proteome of dogs with hypothyroidism were studied using tandem mass tags (TMT) labelling liquid chromatography-mass spectrometry (LC-MS/MS). Saliva from 10 compared healthy dogs. saliva, a total seven proteins showed significant between the two groups, being six downregulated one upregulated, meanwhile, serum, changes, five upregulated. The altered reflected metabolic immunologic as well as, skin coagulation alterations, these not affected by...

Thiobarbituric acid reactive substances (TBARS), carbonyls and oxygen species (ROS) are oxidant compounds that can provide useful information on the oxidative status. Pigs be affected by stress in different situations including physiological conditions such as lactation also diverse diseases, measurement of these three analytes saliva could potentially biomarkers redox status this species. Assays for TBARS spectrophotometry ROS luminol-based chemiluminescence pigs’ were analytically...

Thiobarbituric acid reactive substances (TBARS), carbonyls and oxygen species (ROS) are oxidant compounds that can provide useful information on the oxidative status. Pigs be affected by stress in different situations including physiological conditions such as lactation also diseases, measurement of these three analytes saliva could potentially biomarkers redox status this species. Assays for TBARS spectrophotometry ROS luminol-based chemiluminescence pigs' were analytically validated...

Hypothyroidism is the most commonly diagnosed endocrine disorder in dogs. It produces a deficiency of thyroid hormones which impacts negatively dog's quality life. The objective this study to evaluate possible changes salivary metabolic profile dogs with hypothyroidism. For purpose, targeted metabolomics analysis performed by LC/MS was made saliva samples from group hypothyroidism and healthy Twenty-three metabolites showed significant downregulation between hypothyroid dogs, these...

<title>Abstract</title> <bold>Background:</bold> Hypothyroidism is the most common endocrine disorder diagnosed in dogs, leading to deleterious effects on dog’s life quality. This study aims evaluate changes redox status canine hypothyroidism. For this purpose, a comprehensive panel of antioxidants and oxidants was measured serum saliva 23 dogs with hypothyroidism, 20 non-thyroidal illness 16 healthy dogs. Among antioxidants, cupric reducing antioxidant capacity (CUPRAC), ferric ability...