A5030606872- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Aluminum toxicity and tolerance in plants and animals
- ATP Synthase and ATPases Research
- Pancreatic function and diabetes
- Ion Channels and Receptors
- Neuroscience and Neuropharmacology Research
- Adenosine and Purinergic Signaling
- Plant nutrient uptake and metabolism
- Trace Elements in Health
- Advanced battery technologies research
- Magnesium in Health and Disease
- Lipid Membrane Structure and Behavior
- Cholinesterase and Neurodegenerative Diseases
- Vitamin K Research Studies
- Hemoglobin structure and function
- Medicinal Plants and Neuroprotection
- Cardiovascular Function and Risk Factors
- Cardiomyopathy and Myosin Studies
- Force Microscopy Techniques and Applications
- Signaling Pathways in Disease
- Erythrocyte Function and Pathophysiology
- Endoplasmic Reticulum Stress and Disease
- Calcium signaling and nucleotide metabolism
- Cellular transport and secretion
Consejo Nacional de Investigaciones Científicas y Técnicas
2009-2024
University of Buenos Aires
2011-2024
Instituto de Química y Fisicoquímica Biológicas
2011-2024
Institute of Astronomy and Space Physics
2020
Abstract Metabolic control analysis (MCA) is a promising approach in biochemistry aimed at understanding processes quantitative fashion. Here the contribution of enzymes and transporters to given pathway flux metabolite concentrations determined expressed quantitatively by means numerical coefficients. can be influenced wide variety modulators acting on one or more metabolic steps along pathway. We describe laboratory exercise study regulation human erythrocytes (RBCs). Within framework MCA,...
As recently described by our group, plasma membrane calcium ATPase (PMCA) activity can be regulated the actin cytoskeleton. In this study, we characterize interaction of purified G-actin with isolated PMCA and examine effect during first polymerization steps. measured surface plasmon resonance, directly interacts an apparent 1:1 stoichiometry in presence Ca2+ affinity micromolar range. assessed photoactivatable probe...
The exposure of the plasma membrane calcium pump (PMCA) to surrounding phospholipids was assessed by measuring incorporation photoactivatable phosphatidylcholine analog [125I]TID-PC/16 protein. In presence Ca2+ both calmodulin (CaM) and phosphatidic acid (PA) greatly decreased PMCA. Proteolysis PMCA with V8 protease results in three main fragments: N, which includes transmembrane segments M1 M2; M, M3 M4; C, M5 M10. CaM level fragments M whereas N M. This suggests that conformational changes...
Abstract Our research aimed to elucidate the mechanism by which aurintricarboxylic acid (ATA) inhibits plasma membrane Ca 2+ -ATPase (PMCA), a crucial enzyme responsible for calcium transport. Given pivotal role of PMCA in cellular homeostasis, understanding how it is inhibited ATA holds significant implications potentially regulating physiopathological processes this pump involved. experimental findings revealed that employs multiple modes action inhibit activity, are influenced ATP but...
Plasma membrane Ca2+-ATPase (PMCA) transports Ca2+ by a reaction cycle including phosphorylated intermediates. Calmodulin binding to the C-terminal tail disrupts autoinhibitory interactions, activating pump. To assess conformational changes during cycle, we studied structure of different PMCA states using fluorescent probe, hydrophobic photolabeling, controlled proteolysis and activity.  Our results show that calmodulin binds E2P-like states, dephosphorylation, hydrophobicity in...
The Na+/K+-ATPase is an integral plasma membrane glycoprotein of all animal cells that couples the exchange intracellular Na+ for extracellular K+ to hydrolysis ATP. asymmetric distribution and essential cellular life constitutes physical basis a series fundamental biological phenomena. pumping mechanism explained by Albers-Post model. It involves presence gates alternatively exposing transport sites sides includes occluded states in which both are simultaneously closed. Unlike K+,...
Flavonoids are natural compounds responsible for the health benefits of green tea. Some flavonoids present in tea catechins, among which are: epigallocatechin, epicatechin-3-gallate, epicatechin, catechin and epigallocatechin-3-gallate (EGCG). The latter was found to induce apoptosis, reduce reactive oxygen species, some conditions though others it acts as an oxidizing agent, cell cycle arrest, inhibit carcinogenesis. EGCG also be involved calcium (Ca2+) homeostasis excitable non-excitable...