A5050773941- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Fungal and yeast genetics research
- RNA modifications and cancer
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Transgenic Plants and Applications
- DNA and Nucleic Acid Chemistry
- Lysosomal Storage Disorders Research
- Plant Reproductive Biology
- Steroid Chemistry and Biochemistry
- RNA regulation and disease
- RNA Interference and Gene Delivery
- Genetic factors in colorectal cancer
- Cellular transport and secretion
- Glycogen Storage Diseases and Myoclonus
- GABA and Rice Research
- Plant Molecular Biology Research
- interferon and immune responses
- Fungal Infections and Studies
- Biotin and Related Studies
- Phytoestrogen effects and research
Institute of Bioorganic Chemistry
1999-2020
Kurchatov Institute
2020
Institute of Bioorganic Chemistry
1994-2020
Russian Academy of Sciences
1999-2011
Centre de Génétique Moléculaire
2000-2001
CEA Paris-Saclay
2000-2001
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
1995-2001
National Academy of Sciences of Belarus
1989-1994
National Institute of Mental Health
1994
National Institutes of Health
1993
Four cDNAs encoding human polypeptides hRPB7.0, hRPB7.6, hRPB17, and hRPB14.4 (referred to as Hs10 alpha, beta, Hs8, Hs6, respectively), homologous the ABC10 ABC14.5, ABC23 RNA polymerase subunits Sc10 Sc8, Sc6, respectively) of Saccharomyces cerevisiae, were cloned characterized for their ability complement defective yeast mutants. alpha corresponding Sp10 Schizosaccharomyces pombe can an S. cerevisiae mutant (rpc10-delta::HIS3) in alpha. The peptide sequences are highly conserved...
The initial stage of the biosynthesis steroid hormones in animals occurs mitochondria steroidogenic tissues, where cytochrome P450SCC (CYP11A1) encoded by CYP11A1 gene catalyzes conversion cholesterol into pregnenolone - general precursor all hormones, starting with progesterone. This is missing plants mitochondrial cytochromes P450 (the mito CYP clan) have not been found. Generating transgenic a type from would offer an interesting option to verify whether plant could serve as another site...
Non-canonical DNA structures are widely used for regulation of gene expression, in nanotechnology and the development new DNA-based sensors. I-motifs (iMs) two intercalated parallel duplexes that held together by hemiprotonated C-C base pairs. Previously, iMs were as an accurate sensor intracellular pH measurements. However, iM stability is moderate, which turn limits its vivo applications. Here, we report rational design a substituted phenoxazine 2'-deoxynucleotide (i-clamp) stabilization....
Rpb8p, a subunit common to the three yeast RNA polymerases, is conserved among eukaryotes and absent from noneukaryotes. Defective mutants were found at an invariant GGLLM motif two other highly amino acids. With one exception, they are clustered on Rpb8p structure. They all impair two-hybrid interaction with fragment in largest subunits of polymerases I (Rpa190p), II (Rpb1p), III (Rpc160p). This corresponds pore 1 module polymerase crystal structure bears (P.I.KP..LW.GKQ) facing Rpb8p. An...
ABC10β, a small polypeptide common to the three yeast RNA polymerases, has close homology N subunit of archaeal enzyme and is remotely related smallest vaccinial polymerase. The eucaryotic, archaeal, viral polypeptides share an invariant motif CX 2C… CC that strictly essential for growth, as shown by site-directed mutagenesis, whereas rest ABC10β sequence fairly tolerant amino acid replacements. Zn2+ binding properties in vitro, 2C … may therefore define atypical metal-chelating site. Hybrid...
Rpb11 subunit of RNA polymerase II Eukaryotes is related to N-terminal domain eubacterial α and forms a complex with Rpb3 analogous prokaryotic α2 homodimer, which involved in assembly promoter recognition. In humans, POLR2J gene family has been identified that potentially encodes several hRPB11 proteins differing mainly their short C-terminal regions. The functions the different human specific isoforms are still unknown. To further characterize minor isoform hRPB11bα, only one from (POLR2J)...
Mutations in the CLN3 gene lead to so far an incurable juvenile-onset neuronal ceroid lipofuscinosis (JNCL) or Batten disease that starts at age of 4–6 years with a progressive retinopathy leading blindness. Motor disturbances, epilepsy and dementia manifest during several following years. Most JNCL patients carry same 1.02-kb deletion gene, encoding unusual transmembrane protein, battenin. Based on data genome-wide expression profiling different rate progression [Mol. Med., 2011, 17:...
In Saccharomyces cerevisiae, RNA polymerase II assembly is probably initiated by the formation of RPB3–RPB11 heterodimer. RPB3 encoded a single copy gene in yeast, mouse and human genomes. The RPB11 also unique yeast mouse, but humans family has been identified that potentially encodes several proteins differing mainly their C-terminal regions. We compared abilities both to heterodimerize. show RPB3/RPB11 heterodimer critically depends on presence region RPB11. contrast, tolerates...
As an approach to elucidating the rules governing assembly of human RNA polymerase II (hRPB), interactions between its subunits have been systematically analyzed. Eleven 12 expected hRPB previously tested for reciprocal (J. Biol. Chem. 272 (1997) 16815–16821). We now report results obtained last subunit (hRPB4; Mol. Cell. 18 (1998) 1935–1945) and propose essentially complete picture potential occurring within hRPB. Finally, complementation experiments in yeast indicated that hRPB4 expression...