A5072465275- Genetically Modified Organisms Research
- Food Allergy and Anaphylaxis Research
- Inhalation and Respiratory Drug Delivery
- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Clostridium difficile and Clostridium perfringens research
- Nasolacrimal Duct Obstruction Treatments
- Animal Genetics and Reproduction
- Ophthalmology and Eye Disorders
- Transgenic Plants and Applications
- Animal Nutrition and Physiology
- Botulinum Toxin and Related Neurological Disorders
- Ocular Oncology and Treatments
- Genetic and phenotypic traits in livestock
- Corneal Surgery and Treatments
- Carcinogens and Genotoxicity Assessment
- Cancer and Skin Lesions
- Bacillus and Francisella bacterial research
- Poisoning and overdose treatments
- Radiation Dose and Imaging
- Protein purification and stability
- Hair Growth and Disorders
- Milk Quality and Mastitis in Dairy Cows
- Parasite Biology and Host Interactions
- Coastal and Marine Dynamics
Corteva (United States)
2019-2024
Pioneer Hi-Bred
1995-2018
Human intestinal epithelial cell lines (T84, Caco-2, and HCT-8) grown on permeable Transwell™ filters serve as models of the gastrointestinal barrier. In this study, in vitro model system was evaluated for effectiveness at distinguishing between hazardous non-hazardous proteins. Indicators cytotoxicity (LDH release, MTT conversion), monolayer barrier integrity ([(3)H]-inulin flux, horseradish peroxidase trans-epithelial electrical resistance [TEER]), inflammation (IL-8, IL-6 release) were...
An experimental platform employing human derived intestinal epithelial cell (IEC) line monolayers grown on permeable Transwell® filters was previously investigated to differentiate between hazardous and innocuous proteins. This approach effective at distinguishing these types of proteins perturbation monolayer integrity, particularly transepithelial electrical resistance (TEER), the most sensitive indicator. In current report, in vitro indicators cytotoxicity, inflammation were evaluated...
Relatively few proteins in nature produce adverse effects following oral exposure. Of those that do, are often observed the gut, particularly on intestinal epithelial cells (IEC). Previous studies reported addition of protein toxins to IEC lines disrupted monolayer integrity but innocuous dietary did not. Studies presented here investigated (bovine serum albumin, β-lactoglobulin, RuBisCO, fibronectin) or hazardous (phytohaemagglutinin-E, concanavalin A, wheat germ agglutinin, melittin)...
• Intractable proteins did not produce adverse effects toward human intestinal epithelial cell (IEC) monolayers. do appear to be inherently hazardous as a class of proteins. In vitro methods protein hazard identification require much less than studies with laboratory animals. Inexpensive and readily available cells, reagents, instruments can used for identification.
The ZMM28 protein encoded by the zmm28 gene is endogenous to maize. DP202216 maize was genetically modified increase and extend expression of relative native expression, resulting in plants with enhanced grain yield potential. Evaluation history safe use (HOSU) one component safety assessment framework for a newly expressed GM crop. deduced amino acid sequence introduced identical nonmodified conventional has also been found selected varieties sweet corn kernels, closely related proteins are...
Recent studies suggest that human derived intestinal epithelial cells (IECs) cultured as polarized monolayers on Transwell® filters may respond differently when exposed to hazardous and non-hazardous proteins. This experimental platform was based apical exposure of IEC test proteins for 24 h followed by assessment barrier integrity cell viability. In this study, Caco-2 T84 were evaluated cytotoxicity following 24, 48 72 h. Hazardous included Clostridium difficile toxin A (ToxA), Streptolysin...
Nutritional equivalency feeding studies with diets containing genetically modified grain continue to be required by some regulatory agencies as part of the safety assessment new events. These have long been conducted using broiler chickens, they are very sensitive nutritional deficiencies and antinutrients in diet owing their rapid growth rate. A transgenic maize product event DP-2Ø2216-6 (DP202216) enhanced yield potential an herbicide-tolerance trait has developed. The produced from this...
Feeding studies were conducted with rats and broiler chickens to assess the safety nutrition of maize grain containing event DP-Ø23211-2 (DP23211), a newly developed trait-pyramid product for corn rootworm management. Diets 50% ground from DP23211, non-transgenic control, or reference hybrids (P0928, P0993, P1105) fed Crl:CD®(SD) 90 days. Ross 708 broilers phase diets up 67% each source 42 Body weight, gain, feed conversion determined comparisons between animals DP23211 control in study....
Previous work demonstrated the utility of using human-derived intestinal epithelial cell (IEC) lines cultured as polarized monolayers on Transwell® filters to differentiate between hazardous and non-hazardous proteins. The current study seeks further resolve appropriate concentrations for evaluating proteins unknown hazard potential IEC experimental platform leverages these parameters toxicity insecticidal characteristic those expressed in genetically modified (GM) agricultural biotechnology...
Several regulatory agencies continue to require animal feeding studies approve new genetically modified crops despite such providing little value in the safety assessment. Feeding with maize grain containing event DP-915635-4 (DP915635), a corn rootworm management trait, were conducted fulfill that requirement. Diets fed Crl:CD®(SD) rats for 90 days contained up 50% ground from DP915635, non-transgenic control, or reference hybrids (P1197, 6158, and 6365). Ross 708 broilers received phase...
Recent studies suggest human-derived intestinal epithelial cell (IEC) lines cultured as polarized monolayers on permeable Transwell® filters are effective at differentiating between hazardous and non-hazardous proteins following a single exposure. In this study, IEC were subjected to or in nine exposures over 30 days compared exposure of the same protein. The objective was evaluate whether repeated protein differently alter barrier integrity compromise viability exposures. Proteins tested...