A5086679580- Wheat and Barley Genetics and Pathology
- Food composition and properties
- Celiac Disease Research and Management
- Phytase and its Applications
- Food Allergy and Anaphylaxis Research
- Transgenic Plants and Applications
- Plant Micronutrient Interactions and Effects
- Genetics and Plant Breeding
- Plant Disease Resistance and Genetics
- Agriculture, Plant Science, Crop Management
- Mycotoxins in Agriculture and Food
- Allergic Rhinitis and Sensitization
- Crop Yield and Soil Fertility
- Genetically Modified Organisms Research
- Plant tissue culture and regeneration
- Bioenergy crop production and management
- Chromosomal and Genetic Variations
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Genetic Mapping and Diversity in Plants and Animals
- Plant pathogens and resistance mechanisms
- Potato Plant Research
- Biopolymer Synthesis and Applications
- Plant Genetic and Mutation Studies
- Plant nutrient uptake and metabolism
Università degli Studi della Tuscia
2016-2025
Biopolymères Interactions Assemblages
2017-2019
Deleted Institution
2008
Istituto di Biologia e Biotecnologia Agraria
2008
University of Warwick
2008
Western Regional Research Center
1997-1999
United States Department of Agriculture
1998
Sapienza University of Rome
1987-1989
Abstract Background High amylose starch has attracted particular interest because of its correlation with the amount Resistant Starch (RS) in food. RS plays a role similar to fibre beneficial effects for human health, providing protection from several diseases such as colon cancer, diabetes, obesity, osteoporosis and cardiovascular diseases. Amylose content can be modified by targeted manipulation biosynthetic pathway. In particular, inactivation enzymes involved amylopectin synthesis lead...
Abstract Both high- and low-molecular-weight glutenin subunits (LMW-GS) play the major role in determining viscoelastic properties of wheat (Triticum aestivum L.) flour. To date there has been no clear correspondence between amino acid sequences LMW-GS derived from DNA sequencing those actual present endosperm. We have characterized a particular hexaploid bread wheat, component polymer, which we call 42K LMW-GS, isolated sequenced putative corresponding gene. Extensive obtained directly for...
In Central and Southern Italy, where durum wheat represents one of the most widely cultivated crops, grain filling occurs during Spring, a period characterized by sudden increases in temperature. Wheat proteins are classified into albumins, globulins, prolamins. The nonprolamin fractions include with metabolic activity or structural function. order to investigate consequences heat stress on accumulation mature kernels, Italian cultivar Svevo was subjected two thermal regimes (heat versus...
Wheat and its derived foods are widespread, representing one of the main food sources globally. During last decades, incidence disorders related to wheat has become a global issue for human population, probably linked spread wheat-derived foods. It been ascertained that structural metabolic proteins, like α-amylase/trypsin inhibitors (ATI), involved in onset allergies (bakers' asthma) Non-Coeliac Sensitivity (NCWS). The ATI group exogenous protease inhibitors, which encoded by multigene...
Technological properties of wheat flours are largely governed by the functional storage proteins, comprising single chain gliadins and disulphide bonded polymeric proteins (glutenins, triticins, HMW albumins). This study was conducted (a) to measure rate accumulation different classes (b) monitor polymerization subunits, (c) investigate origin differences in degree arising from allelic at Glu-D1 locus glutenin subunits. Developing grains hexaploid cultivars were collected 3-d intervals...
Among wheat gluten proteins, the α-type gliadins are major responsible for celiac disease, an autoimmune disorder that affects about 1% of world population. In fact, these proteins contain several toxic and immunogenic epitopes trigger onset disease. The a multigene family, encoded by genes located at complex Gli-2 loci.Here, three bread deletion lines (Gli-A2, Gli-D2 Gli-A2/Gli-D2) loci were generated introgression in cultivar Pegaso natural mutations, detected different cultivars....
Water deficit and high temperatures are the main environmental factors which affect both wheat yield technological quality in Mediterranean climate. The aim of study was to evaluate variation gluten protein assembly four durum genotypes relation growing seasons different nitrogen levels. genotypes, Marco Aurelio, Quadrato, Pietrafitta Redidenari, were grown under three levels (36, 90 120 kg ha−1) during two Southern Italy. Significant lower a higher concentration observed year characterized...
We carried out a parallel transcriptional and proteomic comparison of seeds from transformed bread wheat line that overexpresses transgenic low molecular weight glutenin subunit gene relative to the corresponding nontransformed genotype. Proteomic analyses showed that, during seed development, several classes endosperm proteins were differentially accumulated in endosperm. As result strong increase amount protein, endogenous subunit, all subclasses gliadins, metabolic as well...
Cereals contain xylanase inhibitor (XI) proteins which inhibit microbial xylanases and are considered part of the defense mechanisms to counteract pathogens. Nevertheless, in planta evidence for this role has not been reported yet. Therefore, we produced a number transgenic plants constitutively overexpressing TAXI-III, member TAXI type XI that is induced by pathogen infection. Results showed TAXI-III endows wheat with new inhibition capacities. We also correctly secreted into apoplast...
Resistant starch (RS) in foods has positive benefits for potentially alleviating lifestyle diseases. RS is correlated positively with amylose content. This study aimed to see what level of durum wheat needed lower pasta GI. The silencing synthases IIa (SSIIa) and branching enzymes (SBEIIa), key genes involved biosynthesis, cultivar Svevo was performed spaghetti prepared evaluated. SSIIa SBEIIa mutants have a 28% 74% increase 2.8- 35-fold RS, respectively. Cooked softer, higher cooking loss...
Micronutrient malnutrition affects more than half of the world population. Reduced bioavailability microelements in raw materials is considered one main causes mineral deficiency populations whose diet largely based on consumption staple crops. In this context, production low phytic acid ( lpa ) cereals a goal breeding programs, as (PA) binds essential cations such iron (Fe), zinc (Zn), manganese (Mn), potassium (K), calcium (Ca) and magnesium (Mg) precipitating form phytate salts poorly...
Environmental stress during grain filling may affect wheat protein composition, thus influencing its final quality. A proteomic approach was used to evaluate changes in storage composition under water of two Italian durum (Triticum turgidum ssp. durum) cultivars, Ciccio and Svevo. The high-molecular-weight glutenin region increased progressively both cultivars regimens. L48-35 region, corresponding low-molecular-weight (LMW) subunits, slightly development decreased cultivars. In particular,...
Both Glycaemic index (GI) and Load (GL) were introduced to measure the impact of a carbohydrate-containing food on blood glucose. From this perspective, high-amylose (HA) flours, with higher percentage resistant starch (RS), may represent suitable raw material improve glycaemic response. The present work aims investigate GI HA bakery products (biscuits, taralli bread) compared obtained from conventional flour. Ten healthy volunteers enrolled their capillary glucose was measured every 15 min...