A5090270535- Ion Channels and Receptors
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Magnesium in Health and Disease
- Cardiac electrophysiology and arrhythmias
- Helicobacter pylori-related gastroenterology studies
- Plant Stress Responses and Tolerance
- Cell Adhesion Molecules Research
- Microbial Metabolites in Food Biotechnology
- Wound Healing and Treatments
- Veterinary medicine and infectious diseases
- Calpain Protease Function and Regulation
- Connexins and lens biology
- Cellular Mechanics and Interactions
- Biochemical and Molecular Research
- Endoplasmic Reticulum Stress and Disease
- Advanced Proteomics Techniques and Applications
- Chemical Synthesis and Analysis
- Bioactive Compounds and Antitumor Agents
- Bioinformatics and Genomic Networks
- Protein Degradation and Inhibitors
- Mitochondrial Function and Pathology
- Mass Spectrometry Techniques and Applications
- Galectins and Cancer Biology
- Plant and Biological Electrophysiology Studies
University of Chile
2016-2025
Millennium Nucleus of Ion Channel Associated Diseases
2017-2025
Millennium Science Initiative
2020
University of California, Davis
2010-2015
Austral University of Chile
2004
Increased expression of the TRPM 4 channel has been reported to be associated with progression prostate cancer. However, molecular mechanism underlying its effect remains unknown. This work found that decreasing levels leads reduced proliferation PC 3 cells. was a decrease in total β‐catenin protein and nuclear localization, significant reduction Tcf/Lef transcriptional activity. Moreover, silencing increases Ser33/Ser37/Thr41 phosphorylated population reduces phosphorylation GSK ‐3β at...
Spontaneous Ca2+ signaling from the InsP3R intracellular release channel to mitochondria is essential for optimal oxidative phosphorylation (OXPHOS) and ATP production. In cells with defective OXPHOS, reductive carboxylation replaces metabolism maintain amounts of reducing equivalents metabolic precursors. To investigate role mitochondrial uptake in regulating bioenergetics these cells, we used OXPHOS-competent OXPHOS-defective cells. Inhibition activity or increased α-ketoglutarate (αKG)...
Necrosis is associated with an increase in plasma membrane permeability, cell swelling, and loss of integrity subsequent release cytoplasmic constituents. Severe redox imbalance by overproduction reactive oxygen species one the main causes necrosis. Here we demonstrate that H2O2 induces a sustained activity TRPM4, Ca2+-activated, Ca2+-impermeant nonselective cation channel resulting increased vulnerability to death. In HEK 293 cells overexpressing was found eliminate dose-dependent manner...
The Kv2.1 voltage-gated K+ channel is widely expressed throughout mammalian brain, where it contributes to dynamic activity-dependent regulation of intrinsic neuronal excitability. Here we show that somatic plasma membrane clusters are juxtaposed intracellular ryanodine receptor (RyR) Ca2+ -release channels in mouse brain neurons, most prominently medium spiny neurons (MSNs) the striatum. Electron microscopy-immunogold labeling shows MSNs, adjacent subsurface cisternae, placing close...
Cellular migration and contractility are fundamental processes that regulated by a variety of concerted mechanisms such as cytoskeleton rearrangements, focal adhesion turnover, Ca2+ oscillations. TRPM4 is Ca2+-activated non-selective cationic channel (Ca2+-NSCC) conducts monovalent but not divalent cations. Here, we used mass spectrometry-based proteomics approach to identify putative TRPM4-associated proteins. Interestingly, the largest group these proteins has actin cytoskeleton-related...
Breast cancer is one of the most frequent types worldwide and first cause cancer-related deaths in women. Although significant therapeutic advances have been achieved with drugs such as tamoxifen trastuzumab, breast still caused 627,000 2018. Since a multifactorial disease, it has become necessary to develop new molecular therapies that can target several relevant cellular processes at once. Ion channels are versatile regulators physiological- pathophysiological-related mechanisms, including...
Kv1 channels are concentrated at specific sites in the axonal membrane, where they regulate neuronal excitability. Establishing these distributions requires regulated dissociation of from trafficking machinery and their subsequent insertion into membrane. We find that auxiliary Kvβ2 subunit purified brain is phosphorylated on serine residues 9 31, cyclin-dependent kinase (Cdk)–mediated phosphorylation negatively regulates interaction with microtubule plus end–tracking protein EB1. Endogenous...
Transient receptor potential melastatin 4 (TRPM4) is a Ca2+-activated nonselective cationic channel that regulates cell migration and contractility. Increased TRPM4 expression has been related to pathologies, in which cytoskeletal rearrangement are altered, such as metastatic cancer. Here, we identify the K+ tetramerization domain 5 (KCTD5) protein, putative adaptor of cullin3 E3 ubiquitin ligase, novel TRPM4-interacting protein. We demonstrate KCTD5 positive regulator activity by enhancing...
Endothelial damage and angiogenesis are fundamental elements of neovascularisation fibrosis observed in patients with coronavirus disease 2019 (COVID-19). Here, we aimed to evaluate whether early endothelial angiogenic biomarkers detection predicts mortality major cardiovascular events COVID-19 requiring respiratory support. Changes serum syndecan-1, thrombomodulin, factor concentrations were analysed during the first 24 h 10 days after hospitalisation high-flow nasal oxygen or mechanical...
Secreted protein acidic and rich in cysteine (SPARC), or osteonectin, is a matricellular that modulates interactions between cells their microenvironment. SPARC expressed during extracellular matrix remodeling abundant bone marrow high-grade prostate cancer (PCa). In PCa, induces changes associated with epithelial-mesenchymal transition (EMT), enhancing migration invasion increasing the expression of EMT transcriptional factor Zinc finger E-box-binding homeobox 1 (ZEB1), but not SNAI1...
Helicobacter pylori ATCC43504 responds chemotactically to aspartic acid and serine, but not arginine, nor sodium bicarbonate. In contrast, H. ATCC700392 (strain 26695) shows chemotaxis all four attractants. Open reading frame HP0099 from 26695 is predicted encode one of three methyl-accepting receptor proteins (MCPs). When Escherichia coli transformed with a plasmid carrying strain 26695, the recombinants acquire bicarbonate, urea. 43504, gene interrupted mini-IS605 insertion, which accounts...
Several mechanisms of immune suppression have been attributed to Foxp3+ T regulatory cells (Treg) including modulation target via inhibition cell proliferation, alteration cytokine secretion, and modification phenotype, among others. Neuropilin-1 (Nrp1), a co-receptor protein highly expressed on Treg has involved in tolerance-mediated responses, driving tumor growth, transplant acceptance. Here, we extend our previous findings showing that, despite expressing Foxp3, Nrp1KO deficient...
Dysregulation of Transient Receptor Potential Canonical 6 (TRPC6) channel is associated with pathologies in which cell contraction relevant. Therefore, understanding the molecular mechanisms underlying regulation actin cytoskeletal function by TRPC6 important. Here, we observed that upregulates activity RhoA GTPase, affecting organization and polymerization cytoskeleton focal adhesion dynamics. Moreover, promoted migration. Using mass spectrometry, identified Rhotekin‐1 (RTKN‐1), an effector...
A study was conducted on the occurrence of 2 Diphyllobothrium species in 5 fish from Lake Panguipulli southern Chile and dogs humans associated with lake. Fish were examined for plerocercoid larvae, human dog feces towns Choshuenco sampled presence tapeworm ova. Odonthestes (Cauque) mauleanum (Steindachner, 1896) is reported first time as a host both latum Linnaeus, 1758, dendriticum (Nitzsch, 1824). Basilichthys australis Eigenmann, 1927, D. latum. Prevalences mean intensities higher...
About half of the human population is infected with Helicobacter pylori, a bacterium causing gastritis, peptic ulcer and progression to gastric cancer.Chemotaxis fl agellar motility are required for colonization persistence H. pylori in mucus layer.It not completely clear which chemical gradients used by maintain its position.TlpA, chemotaxis receptor arginine/ bicarbonate, has been identifi ed.This study aimed fi nd out whether tlpA gene expression chemotactic response arginine/bicarbonate....