Cellulose, an abundant, crystalline polysaccharide, is central to plant morphogenesis and many industries. Chemical ultrastructural analyses together with map-based cloning indicate that the RSW1 locus of Arabidopsis encodes catalytic subunit cellulose synthase. The cloned gene complements rsw1 mutant whose temperature-sensitive allele changed in one amino acid. causes a specific reduction synthesis, accumulation noncrystalline β-1,4-glucan, disassembly synthase, widespread morphological...

A rapidly growing world population has highlighted the need to significantly increase food production in context of a with accelerating soil and water shortages as well climatic stressors. This situation generated new interest application liquid seaweed extracts because their potent plant growth-enhancing properties through metabolic benefits, triggering disease response pathways increasing stress tolerance. The basis for these benefits is complex poorly understood. Liquid are have been...

Abstract An 8.5-kb cosmid containing the KORRIGAN gene complements cellulose-deficient rsw2-1 mutant of Arabidopsis. Three temperature-sensitive alleles rsw2show single amino acid mutations in putative endo-1,4-β-glucanase encoded by KOR. The F1 from crosses betweenkor-1 and rsw2 shows a weak, root phenotype. shoots ofrsw2-1 seedlings produce less cellulose accumulate short chain, readily extractable glucan resembling that reported forrsw1 (which is defective glycosyltransferase required for...

Abstract This study investigated the effects of seaweed extract (SWE) made from brown algae Durvillaea potatorum and Ascophyllum nodosum on plants soil. The application SWE to soil growing tomato showed dual effects. comprehensively improved plant growth (flower clusters, flower number, fruit root length, shoot dry weight, SPAD) increased productivity (yield quality). Similarly, effected biology at zone by increasing total bacterial count available nitrogen impacting community diversity with...

Summary rsw3 is a temperature‐sensitive mutant of Arabidopsis thaliana showing radially swollen roots and deficiency in cellulose. The gene was identified by map‐based strategy, shows high similarity to the catalytic α‐subunits glucosidase II from mouse, yeast potato. These enzymes process N‐linked glycans ER, so that they bind then release chaperones as part quality control pathway, ensuring correct protein folding. Putative β‐subunits for holoenzyme rice genomes share characteristic motifs...

A global gene expression profiling study at different stages of fiber development was undertaken on two cotton species cultivated for fiber, Gossypium hirsutum (L.) and G. barbadense (L.). large proportion the genome expressed during both elongation subsequent secondary cell wall thickening. There a major shift in abundance transcripts regulation, organization metabolism between thickening that fundamentally similar species. Each stage had its own distinctive features represented by specific...

Abstract Sucrose (Suc) synthase (Sus) is the major enzyme of Suc breakdown for cellulose biosynthesis in cotton (Gossypium hirsutum) fiber, an important source fiber textile industry. This study examines tissue-specific expression, relative abundance, and temporal expression various Sus transcripts proteins present cotton. A novel isoform (SusC) identified that expressed at high levels during secondary cell wall synthesis fraction. The phylogenetic relationships deduced amino acid sequences...

This study investigated whether kelp extract from Durvillaea potatorum and Ascophyllum nodosum (Seasol Commercial®) stimulates broccoli establishment growth. Under controlled conditions in the glasshouse, weekly applications of significantly increased leaf area, stem diameter biomass by up to 70%, 65% 145%, respectively. Also lower strength dilutions (1:200 1:500) were most effective stimulating early growth broccoli, whereas higher (1:25 1:100) later plant development. In field, application...

The withdrawal of soil fumigants like methyl bromide is forcing strawberry growers to consider supplementary and alternative ways producing crops. In addition controlling soil-borne pests, fumigation causes an increased growth response in roots, the use biostimulants may offer replace this response. We tested hypothesis that treatment with a commercial extract (Seasol®) from seaweeds Duvillaea potatorum Ascophyllum nodosum can increase root growth, transplant (runner) fruit yields...

Dynamin-related proteins are large GTPases that deform and cause fission of membranes. The DRP1 family Arabidopsis thaliana has five members which DRP1A, DRP1C, DRP1E widely expressed. Likely functions DRP1A were identified by studying rsw9, a null mutant the Columbia ecotype grows continuously but with altered morphology. Mutant roots hypocotyls short swollen, features plausibly originating in their cellulose-deficient walls. reduction cellulose is specific since non-cellulosic...

Seaweed extracts are important sources of plant biostimulants that boost agricultural productivity to meet current world demand. The ability seaweed based on either the Phaeophyceaean species Ascophyllum nodosum or Durvillaea potatorum enhance growth suppress disease have recently been shown. However, very limited information is available mechanisms suppression by such extracts. In addition, there no a combination from A. and D. pathogen induce defence. present study has explored...

Abstract Seaweed extracts are agricultural biostimulants that have been shown to increase the productivity of many crops. The aim this study was determine effect a seaweed extract from brown algae Durvillaea potatorum and Ascophyllum nodosum as soil treatment on yield wine grapes grown in Australian production climate conditions. This used series seven field experiments (2012–2017), across five locations, three states four cultivars, analysed data using linear mixed model approach. analysis...

Abstract Seaweed extracts are proven to increase productivity in many agricultural crops, but there is limited research on their use avocado production. Therefore, we evaluated the effectiveness of a seaweed extract from Durvillaea potatorum and Ascophyllum nodosum yield, revenue post-harvest fruit quality series field experiments Australia, seedling root growth pot experiment. The were conducted commercial farms across three different locations northern Queensland over four years utilised...

Seaweed extracts are a prominent class of biostimulants that enhance plant health and tolerance to biotic abiotic stresses due their unique bioactive components. However, the mechanisms action still unknown. Here, we have used metabolomic approach, UHPLC-MS method, uncover induced following application Arabidopsis thaliana seaweed extract derived from Durvillaea potatorum Ascophyllum nodosum. We identified, extract, key metabolites systemic responses in roots leaves across 3 timepoints (0,...

Summary The Arabidopsis radial swelling mutant rsw10 showed ballooning of root trichoblasts, a lower than wild‐type level cellulose and altered levels some monosaccharides in non‐cellulosic polysaccharides. Map‐based cloning that the mutated gene ( At1g71100 ) encodes ribose 5‐phosphate isomerase (RPI) mutation replaces conserved glutamic acid residue with lysine. Although RPI is intimately involved many biochemical pathways, media supplementation experiments suggest visible phenotype...

A sand solution technique demonstrated the capacity for a commercial seaweed extract from

The pursuit of sustainable and productive agriculture demands the exploration innovative approaches to improve plant productivity soil health. utilization natural agricultural biostimulants, such as extracts from seaweed, fish, humus, has gained prominence an ecological strategy achieve this goal. In study we investigated effectiveness a fortified biostimulant extract (FBE), composed on tomato physiology, productivity, growing media properties, estimated carbon emissions associated with...

Expression patterns of two clover chalcone synthase promoters (TsCHS1 and TsCHS3) were examined in transgenic Trifolium subterraneum plants to investigate flavonoid gene regulation determine if there is differential these from the CHS multigene family. The CHS1 CHS3 genes occur as part a tightly linked cluster tandemly orientated subterranean clover. fused β-glucuronidase (GUS) reporter reintroduced into clovers. Analysis expression showed that near-identical occurred containing either...