A5084675923- Polysaccharides and Plant Cell Walls
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Advanced Cellulose Research Studies
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Biofuel production and bioconversion
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Microbial Metabolites in Food Biotechnology
- Plant Stress Responses and Tolerance
- Enzyme Production and Characterization
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Cellular transport and secretion
- Genomics and Chromatin Dynamics
- Plant Physiology and Cultivation Studies
- Transgenic Plants and Applications
- Plant Genetic and Mutation Studies
- Cell Image Analysis Techniques
- Morphological variations and asymmetry
- Phytase and its Applications
AgroParisTech
2010-2025
Université Paris-Saclay
2016-2025
Institut Jean-Pierre Bourgin
2015-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021-2025
Centre National de la Recherche Scientifique
2014-2019
Institut National de la Recherche Agronomique
1995-2014
BOKU University
2014
Graduate School Experimental Plant Sciences
2012
Stanford University
2012
Wageningen University & Research
2012
In all land plants, cellulose is synthesized from hexameric plasma membrane complexes. Indirect evidence suggests that in vascular plants the complexes involved primary wall synthesis contain three distinct synthase catalytic subunits (CESAs). this study, we show CESA3 and CESA6 fused to GFP are expressed same cells at time hypocotyl of etiolated seedlings migrate with comparable velocities along linear trajectories cell surface. We also can be coimmunoprecipitated detergent-solubilized...
Mutants at the PROCUSTE1 (PRC1) locus show decreased cell elongation, specifically in roots and dark-grown hypocotyls. Cell elongation defects are correlated with a cellulose deficiency presence of gapped walls. Map-based cloning PRC1 reveals that it encodes member (CesA6) synthase catalytic subunit family, which least nine other members exist Arabidopsis. Mutations another family member, RSW1 (CesA1), cause similar wall all types, including those hypocotyls roots, suggesting synthesis these...
Abstract Plant growth and organ formation depend on the oriented deposition of load-bearing cellulose microfibrils in cell wall. Cellulose is synthesized by plasma membrane–bound complexes containing synthase proteins (CESAs). Here, we establish a role for cytoskeleton intracellular trafficking (CSCs) through vivo study green fluorescent protein (GFP)-CESA3 fusion Arabidopsis thaliana hypocotyls. GFP-CESA3 localizes to membrane, Golgi apparatus, compartment identified VHA-a1 marker, and,...
Plants actively perceive and respond to perturbations in their cell walls which arise during growth, biotic abiotic stresses. However, few components involved plant wall integrity sensing have been described date. Using a reverse-genetic approach, we identified the Arabidopsis thaliana leucine-rich repeat receptor kinase MIK2 as an important regulator of damage responses triggered upon cellulose biosynthesis inhibition. Indeed, loss-of-function mik2 alleles are strongly affected immune...
Abstract An 8.5-kb cosmid containing the KORRIGAN gene complements cellulose-deficient rsw2-1 mutant of Arabidopsis. Three temperature-sensitive alleles rsw2show single amino acid mutations in putative endo-1,4-β-glucanase encoded by KOR. The F1 from crosses betweenkor-1 and rsw2 shows a weak, root phenotype. shoots ofrsw2-1 seedlings produce less cellulose accumulate short chain, readily extractable glucan resembling that reported forrsw1 (which is defective glycosyltransferase required for...
Post-transcriptional gene silencing (cosuppression) results in the degradation of RNA after transcription. A transgenic Arabidopsis line showing post-transcriptional a 35S–uidA transgene and uidA-specific methylation was mutagenized using ethyl methanesulfonate. Six independent plants were isolated which uidA mRNA accumulation β-glucuronidase activity increased up to 3500-fold, whereas transcription rate only threefold. These each carried recessive monogenic mutation that is responsible for...
The cell wall is the major limiting factor for plant growth. Wall extension thought to result from loosening of its structure. However, it not known how this coordinated with synthesis. We have identified two novel allelic cellulose-deficient dwarf mutants, kobito1-1 and kobito1-2 (kob1-1 kob1-2). cellulose deficiency was confirmed by direct observation microfibrils in most recent layers elongating root cells. In contrast wild type, which showed transversely oriented parallel microfibrils,...
Plant growth and organ formation depend on the oriented deposition of load-bearing cellulose microfibrils in cell wall. Cellulose is synthesized by a large relative molecular weight synthase complex (CSC), which comprises at least three distinct synthases. synthesis plants or bacteria also requires activity an endo-1,4-β-d-glucanase, exact function process not known. Here, we show, to our knowledge for first time, that leaky mutation Arabidopsis (Arabidopsis thaliana) membrane-bound...
Despite an ever-increasing interest for the use of pectin-derived oligogalacturonides (OGs) as biological control agents in agriculture, very little information exists-mainly technical reasons-on nature and activity OGs that accumulate during pathogen infection. Here we developed a sensitive OG profiling method, which revealed unsuspected features generated infection Arabidopsis thaliana with fungus Botrytis cinerea Indeed, contrast to previous reports, most were acetyl- methylesterified,...
Abstract Cellulose synthesis is driven by large plasma membrane-inserted protein complexes, which in plants have 6-fold symmetry. In Arabidopsis (Arabidopsis thaliana), functional cellulose complexes (CSCs) are composed of at least three different synthase catalytic subunits (CESAs), but the actual ratio CESA isoforms within CSCs remains unresolved. this work, stoichiometry CESAs primary cell wall CSC was determined, after elimination redundancy a mutant background, coimmunoprecipitation and...
Abstract The synthesis of cellulose microfibrils requires the presence a membrane-bound endo-1,4-β-d-glucanase, KORRIGAN1 (KOR1). Although exact biochemical role KOR1 in is unknown, we used protein as marker to explore potential involvement subcellular transport processes synthesis. Using immunofluorescence and green fluorescent (GFP)–KOR1 fusion that complemented phenotype conferred by kor1-1 mutant, investigated distribution epidermal cells root meristem. was localized intracellular...
During cytokinesis a new crosswall is rapidly laid down. This process involves the formation at cell equator of tubulo-vesicular membrane network (TVN). TVN evolves into tubular (TN) and planar fenestrated sheet, which extends its periphery before fusing to mother wall. The role wall polymers in plate assembly poorly understood. We used specific stains GFP-labelled cellulose synthases (CESAs) show that cellulose, as well three distinct CESAs, accumulated already stage. early presence...
Abstract In higher plants, cellulose is synthesized by so-called rosette protein complexes with synthases (CESAs) as catalytic subunits of the complex. The CESAs are divided into two distinct families, three which thought to be specialized for primary cell wall and secondary wall. this article, potential forming a functional complex has been investigated. membrane-based yeast two-hybrid biomolecular fluorescence systems were used assess interactions between (CESA1, CESA3, CESA6), (CESA4,...
Plant shoots have thick, polylamellate outer epidermal walls based on crossed layers of cellulose microfibrils, but the involvement microtubules in such wall lamellation is unclear. Recently, using a long-term movie system which Arabidopsis seedlings were grown biochamber, tracks along cortical move shown to undergo slow rotary movements over surface hypocotyl cells. Because are known guide synthases short term, we hypothesised that this previously unsuspected microtubule rotation could,...
In higher plants, cellulose is synthesized by plasma membrane-localized synthase complexes (CSCs). Arabidopsis thaliana GH9A1/KORRIGAN1 a membrane-bound, family 9 glycosyl hydrolase that important for synthesis in both primary and secondary cell walls. Most previously identified korrigan1 mutants show severe phenotypes such as embryo lethality; therefore, the role of GH9A1 remains unclear. Here, we report novel A577V missense mutation, designated jiaoyao1 (jia1), second active site signature...
MINT‐6951199: CESA8 (uniprotkb:Q8LPK5) physically interacts (MI:0218) with by bimolecular fluorescence complementation (MI:0809)
The oriented deposition of cellulose microfibrils in the plant cell wall plays a crucial role various functions such as growth, organ formation and defense responses. Cellulose is synthesized by synthase complexes (CSCs) embedded plasma membrane (PM), which comprise synthases (CESAs). abundance localization CSCs at PM should be strictly controlled for precise regulation deposition, strongly depends on trafficking system. However, mechanism intracellular transport still poorly understood. In...
Because the plant cell wall provides first line of defense against biotic and abiotic assaults, its functional integrity needs to be maintained under stress conditions. Through a phenotype-based compound screening approach, we identified novel cellulose synthase inhibitor, designated C17. C17 administration depletes complexes from plasma membrane in Arabidopsis thaliana, resulting anisotropic elongation weak wall. Surprisingly, addition mutations CELLULOSE SYNTHASE1 (CESA1) CESA3, forward...
Cellulose is synthesized by the so called rosette protein complex and catalytic subunits of this are cellulose synthases (CESAs). It thought that complexes in primary secondary cell walls each contains at least three different non-redundant synthases. In addition to CESA proteins, biosynthesis almost certainly requires action other although few have been identified little known about biochemical role those identified. One these proteins KORRIGAN (KOR1). Mutant analysis Arabidopsis thaliana...
Cellulose synthase complexes (CSCs) at the plasma membrane (PM) are aligned with cortical microtubules (MTs) and direct biosynthesis of cellulose. The mechanism interaction between CSCs MTs, cellular determinants that control delivery PM, not yet well understood. We identified a unique small molecule, CESA TRAFFICKING INHIBITOR (CESTRIN), which reduces cellulose content alters anisotropic growth Arabidopsis (Arabidopsis thaliana) hypocotyls. monitored distribution mobility fluorescently...