A5078142325- Plant Molecular Biology Research
- Plant Reproductive Biology
- Polysaccharides and Plant Cell Walls
- Plant nutrient uptake and metabolism
- Forest ecology and management
- Wood Treatment and Properties
- Biofuel production and bioconversion
- Plant and Biological Electrophysiology Studies
- Algal biology and biofuel production
- Enzyme Production and Characterization
- Forest Biomass Utilization and Management
- Advanced Cellulose Research Studies
- Marine and coastal plant biology
- Studies on Chitinases and Chitosanases
- Tree Root and Stability Studies
- Enzyme Catalysis and Immobilization
- Animal Disease Management and Epidemiology
- Seaweed-derived Bioactive Compounds
- Agriculture and Rural Development Research
- Microbial Community Ecology and Physiology
- Wood and Agarwood Research
- Plant Stress Responses and Tolerance
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2019-2024
Université Claude Bernard Lyon 1
2019-2024
Laboratoire Reproduction et Développement des Plantes
2019-2024
École Normale Supérieure de Lyon
2019-2024
Centre National de la Recherche Scientifique
2019-2024
Institut Jean-Pierre Bourgin
2014-2022
AgroParisTech
2016-2022
Université Paris-Saclay
2016-2022
Fudan University
2022
Université de Rennes
2022
To relate gene networks and organ shape, one needs to address two wicked problems: i) Gene expression is often variable locally, shape reproducible globally; ii) can have cascading effects on tissue mechanics, with possibly counterintuitive consequences for the final shape. Here, we such problems, taking advantage of simpler plant development where only emerges from cell division elongation. We confirm that mutation in VERNALIZATION INDEPENDENCE 3 ( VIP3 ), a subunit conserved...
Perturbation of cellulose synthesis in plants triggers stress responses, including growth retardation, mediated by the cell wall integrity-sensing receptor-like kinase (RLK) THESEUS1 (THE1). The analysis two alleles carrying T-DNA insertions at comparable positions has led to conflicting conclusions concerning impact THE1 signaling on growth. Here we confirm that, unlike the1-3 and other the1 which cellular responses genetic or pharmacological inhibition are attenuated, the1-4 showed...
A major challenge in plant systems biology is the development of robust, predictive multiscale models for organ growth. In this context it important to bridge gap between the, rather well-documented molecular scale and by providing quantitative methods study within-organ growth patterns. Here, we describe a simple method analysis evolution patterns within rod-shaped organs that does not require adding markers at surface. The allows simultaneous root hypocotyl growth, provides spatio-temporal...
The shoot apical meristem (SAM) gives rise to all aerial plant organs. Cell walls are thought play a central role in this process, translating molecular regulation into dynamic changes growth rate and direction, although their precise morphogenesis during organ formation is poorly understood. Here, we investigated the of xyloglucans (XyGs), major, yet functionally characterized, wall component SAM Arabidopsis (Arabidopsis thaliana). Using immunolabeling, biochemical analysis, genetic...
The de-methylesterification of the pectic polysaccharide homogalacturonan (HG) by pectin methylesterases (PMEs) is a critical step in control plant cell expansion and morphogenesis. Plants have large gene families encoding PMEs but also PME inhibitors (PMEIs) with differ their biochemical properties. Arabidopsis thaliana PECTIN METHYLESTERASE INHIBITOR 3 (PMEI3) frequently used as tool to manipulate methylesterase activity studies assessing its role One limitation these that exact this...
Although the formation of new walls during plant cell division tends to follow maximal tensile stress direction, analyses individual cells over time reveal a much more variable behavior. The origin such variability as well exact role interphasic microtubule behavior before have remained mysterious so far. To approach this question, we took advantage Arabidopsis stem, where pattern is both highly anisotropic and stable. cortical microtubules (CMTs) generally align with stress, detected...
Abstract In contrast to animal cells with their contractile cortical cytoskeleton, plant are encased in walls and usually thought behave like stiff, pressurized vessels. When digesting the cell wall, protoplasts form spherical shapes, further consolidating this model. Here we challenge apparent opposition between cells. We show that can protrusions. More precisely, using plasma membrane markers, reveal 27 ± 1% of dot-like protrusions 16 7% long (typically 1-20 μm, up 200 μm) name “filopods”....
ABSTRACT The shoot apical meristem (SAM) gives rise to all aerial organs of the plant. cell walls are supposed play a central role in this process, translating molecular regulation into dynamic changes growth rates and directions, although their precise morphogenesis during organ formation remains not well understood. Here we investigate xyloglucans (XyGs), which form major, yet functionally poorly characterized, wall component SAM. Using immunolabeling, biochemical analysis, genetic...
SUMMARY In multicellular systems, all cells exhibit transcriptional noise. However, its exact contribution to morphogenesis often remains unclear, especially in animals where can also move. Here we take advantage of walled plant cells, noise happens tissues with a fixed topology. Using synchronously growing guard stomata, demonstrate that mutation VIP3 , subunit the conserved polymerase-associated factor 1 complex (Paf1C), increases Arabidopsis. This conclusion could be generalized other...
The de-methylesterification of the pectic polysaccharide homogalacturonan (HG) by pectin methylesterases (PMEs) is a critical step in control plant cell expansion and morphogenesis. Plants have large gene families encoding PMEs but also PME inhibitors (PMEIs) with differ their biochemical properties. Arabidopsis thaliana PECTIN METHYLESTERASE INHIBITOR 3 (PMEI3) frequently used as tool to manipulate methylesterase activity studies assessing its role One limitation these that exact this...
Abstract The Arabidopsis thaliana PECTIN METHYLESTERASE INHIBITOR 3 (PMEI3) gene is frequently used as a tool to manipulate PME activity in vivo , studies assessing the role of pectin de-methylesterification control cell expansion. One limitation these that exact biochemical this protein has not yet been determined. In manuscript we produced Pichia pastoris and characterized its vitro . Like other PMEIs, PMEI3 inhibits acidic pH conditions for variety wall extracts purified preparations, but...