Abstract Organo-metal halide perovskites are promising solution-processed semiconductors, however, they possess diverse and largely not understood non-radiative mechanisms. Here, we resolve contributions of individual recombination centers (quenchers) in nanocrystals methylammonium lead iodide by studying their photoluminescence blinking caused random switching quenchers between active passive states. We propose a model to describe the observed reduction upon cooling determine energetic...

Fundmental comprehension of light-induced processes in perovskites are still scarce. One active debate surrounds the influence excess lead iodide (PbI2) on device performance, as well optoelectronic properties, where both beneficial and detrimental traits have been reported. Here, we study its impact charge carrier recombination kinetics by simultaneously acquiring photoluminescence quantum yield time-resolved a function excitation wavelength (450–780 nm). The presence PbI2 perovskite film...

Cells continuously sense external forces from their microenvironment, the extracellular matrix (ECM). In turn, they generate contractile forces, which stiffen and remodel this matrix. Although bidirectional mechanical exchange is crucial for many cell functions, it remains poorly understood. Key challenges are that majority of available matrices such studies, either natural or synthetic, difficult to control lack biological relevance. Here, we use a yet highly biomimetic hydrogel based on...

The structural features of the matrix surrounding cells play a crucial role in regulating their behavior.

Conjugated polymers have been widely studied as flexible, versatile semiconductors in organic electronics. However, the material stability is one of problems limiting their applications. Thus, understanding degradation process conjugated crucial. In this work, we monitored chain scission model polymer MEH-PPV chloroform solutions under different conditions by assessing its molecular weight using gel permeation chromatography and optical spectral measurements. We showed that changes UV-VIS...

Multifocal plane microscopy allows for capturing images at different focal planes simultaneously. Using a proprietary prism which splits the emitted light into paths of lengths, 8 depths were obtained, covering volume 50x50x4 µm3. The position single emitters was retrieved using phasor-based approach across imaging planes, with better than 10 nm precision in axial direction. We validated accuracy this by tracking fluorescent beads 3D to calculate water viscosity. fast acquisition rate (>100...

Laser trapping at an interface provides a unique platform for assembling novel multiparticle-based optical matter that extends well beyond the irradiated area. Optical binding, resulting from resonantly scattered photons by gold nanoparticles through dipolar scattering mode, serves as primary force supporting cohesion of particles in these optically induced assemblies, which is interpreted view formation binding network. Unfortunately, dipole mode restricted to narrow range experimental...

The optical resonance between an absorbing particle and the trapping laser can enhance radiation force exerted on micro/nanoscale objects. However, exact mechanism behind this is still elusive. To unravel phenomenon, we studied a single dye-doped polystyrene 1064 nm under specifically designed conditions. was trapped at water–glass interface while simultaneously being excited by 488 widefield laser. In contrast with former reports (∼10–35% stiffness enhancement), obtained unprecedented...

Abstract Optical binding has recently gained considerable attention because it enables the light-induced assembly of many-body systems; however, this phenomenon only been described between directly irradiated particles. Here, we demonstrate that optical can occur outside focal spot a single tightly focused laser beam. By trapping at an interface, assemble up to three gold nanoparticles with linear arrangement which fully-occupies focus. The is efficiently scattered by alignment and interacts...

As the field of metal halide perovskites matures, a range compositionally different perovskite films has found place in efficient optoelectronic devices. These feature variable local structural stability, carrier diffusion, and recombination, while there is still lack easy-to-implement generic protocols for high-throughput characterization these properties. Correlation clustering imaging (CLIM) recently developed tool that resolves peculiarities photophysics by assessing dynamics...

Nanoparticles (NPs) are a promising tool for cancer therapy, yet few have successfully reached clinical application. Current nanomedicine development pipelines focused on optimizing physical properties of NPs, overlooking the impact tumor biology their behavior. Here, we show that same NPs exhibit distinct accumulation and penetration patterns in 3D spheroids derived from four models (representative lung, colon, breast, cervical cancer). We uncover an inverse relationship between NP uptake...

The high stability and conductivity of 3,4-disubstituted polythiophenes such as poly(3,4-ethylenedioxythiophene) (PEDOT) make them attractive candidates for commercial applications. However, next-generation nanoelectronic devices require novel macromolecular strategies the precise synthesis advanced polymer structures well their arrangement. In this report, we present a synthetic route to ladder-like brushes with poly(3,4-propylenedioxythiophene) (PProDOT)-conjugated chains. were prepared...

Mapping of the spatial and temporal motion particles inside an optical field is critical for understanding further improvement 3D spatio-temporal control over their trapping dynamics. However, it not trivial to capture motion, most imaging systems only a 2D projection in which information about axial movement directly available. In this work, we resolve incorporation trajectories 200 nm fluorescent polystyrene site under different experimental conditions using recently developed widefield...

Abstract Interactions between epigenetic readers and histone modifications play a pivotal role in gene expression regulation aberrations can enact etiopathogenic roles both developmental acquired disorders like cancer. Typically, interactions are studied by mass spectrometry or chromatin immunoprecipitation sequencing. However, these methods, spatial information is completely lost. Here, we devise an expansion microscopy based method, termed Expansion Microscopy for Epigenetics ExEpi, to...

Understanding the relationship between active material's structure and function in operational devices is key for rational engineering of next generation devices. However, this has proven elusive due to invasiveness structural characterization methods their inability penetrate multi-layered device. Here, we introduce a Correlation Clustering Imaging Method (CLIM) based on photoluminescence microscopy. CLIM offers insights into functional aspects related carrier transport, defects,...

Optical matter, a transient arrangement formed by the interaction of light with micro/nanoscale objects, provides responsive and highly tunable materials that allow for controlling manipulating and/or matter. A combined experimental theoretical exploration optical matter is essential to advance our understanding phenomenon potentially design applications. Most studies have focused on nanoparticles composed single material (either metallic or dielectric), representing two extreme regimes, one...

Abstract The instability of metal halide perovskites limits the commercialization solar cells despite their impressive efficiencies. This instability, driven by photo‐induced ion migration, leads to material restructuring, defect formation, degradation, and healing. However, these same “unwanted” properties enable propose Correlation Clustering Imaging (CLIM), a technique that detects local photoluminescence (PL) fluctuations through wide‐field fluorescence microscopy. It is shown such are...

Abstract The fate of excited charge carriers in metal halide perovskite semiconductors is influenced by energetic disorder and defects. Here, photoluminescence (PL) blinking used to probe metastable nonradiative (NR) centers the nanoscale energy landscape. Temporal activation an NR center creates a local region with increased recombination. Activation deactivation this PL quenching does not only lead blinking, but also fluctuations spectra, if crystal inhomogeneous sense that emission...

The self-organization of colloidal nanoparticles into complex structures, both in equilibrium and out-of-equilibrium, is a critical area science with potential applications creating new functional materials. While assemblies yield thermodynamically stable periodic non-equilibrium (or active) exhibit dynamic, reconfigurable behavior response to external stimuli. As consequence, understanding the structure-function relationships these remain challenging due their transient, highly dynamic...

Optical trapping and assembling dynamics of polystyrene microparticles (MPs) 1 μm in diameter are studied at its solution–air surface using a widefield microscope. Upon switching on the intense 1064 nm laser, MPs gathered, forming single concentric circle (CC)-like assembly larger than focus. It consists few tens MPs, central part shows structural color, which indicates that is also growing axial direction. The dynamically fluctuating assembly, some them ejected when newly coming collide...

The resonance between an electronic transition of a micro/nanoscale object and incident photon flux can modify the radiation force exerted on that object, especially at interface. It has been theoretically proposed non-linear stimulated emission process also induce optical force, however its direction will be opposite to conventional scattering/absorption processes. In this work, we experimentally demonstrate repulsive pulling single trapped dye-doped particle. Moreover, successfully...

Abstract The interactions between cells and their direct environment are crucial for cell fate but biochemically mechanically highly complex, therefore, poorly understood. Despite recent advances that exposed the impact of a range different factors, real progress remains challenging, since appropriate controllable matrices quantitative analysis techniques cover time length scales unavailable. Here, we use synthetic fibrous hydrogel with nonlinear mechanics to mimic tailor bi-directional...