A5089038469- Vector-Borne Animal Diseases
- Genetic diversity and population structure
- Animal Disease Management and Epidemiology
- Biological Control of Invasive Species
- Viral Infections and Vectors
- Identification and Quantification in Food
- Amphibian and Reptile Biology
- Plant Pathogens and Fungal Diseases
- Plant Taxonomy and Phylogenetics
- Phytoplasmas and Hemiptera pathogens
- Advances in Cucurbitaceae Research
- Insect behavior and control techniques
- Entomopathogenic Microorganisms in Pest Control
- Botany, Ecology, and Taxonomy Studies
- Animal Behavior and Reproduction
- Pacific and Southeast Asian Studies
- Cocoa and Sweet Potato Agronomy
- Viral gastroenteritis research and epidemiology
- Genomics and Phylogenetic Studies
- Cassava research and cyanide
- Insect Resistance and Genetics
- Crustacean biology and ecology
- Plant Pathogenic Bacteria Studies
- Insect-Plant Interactions and Control
- Insect and Pesticide Research
Wagga Wagga Base Hospital
2010-2024
New South Wales Department of Primary Industries
2010-2023
Charles Sturt University
2013-2018
Government of New South Wales
2014
Purdue University West Lafayette
2006-2009
Google (United States)
2008
Griffith University
1999-2002
Culicoides spp. biting midges transmit bluetongue virus (BTV), the aetiological agent of (BT), an economically important disease ruminants. In southern India, hyperendemic outbreaks BT exert high cost to subsistence farmers in region, impacting on sheep production. Effective monitoring methods coupled with accurate species identification can accelerate responses for minimising outbreaks. Here, we assessed utility sampling and DNA barcoding detection order provide informed basis future their...
The biting midge fauna of Dongzhaigang Mangrove Forest, Hainan Province, China was sampled on 14 October 2015 using three methods: a pan light trap operated from dusk until dawn the following morning and sweep net human landing collections performed between 16:15–17:15 hr. Eight species, including two new records for China, Culicoides palawanensis C. niphanae, one record Hainan, circumbasalis, were collected. A key to assist with identification specimens these species is provided. DNA...
Background Detecting and controlling the movements of invasive species, such as insect pests, relies upon rapid accurate species identification in order to initiate containment procedures by appropriate authorities. Many tussock moth genus Lymantria are significant forestry including gypsy dispar L., consequently have been a focus for development molecular diagnostic tools assist identifying source populations. In this study we expand taxonomic geographic coverage DNA barcode reference...
Major histocompatibility complex (MHC) alleles likely have adaptive value because of overdominance, in which case MHC heterozygous individuals increased fitness relative to homozygotes. Because this potential benefit, the evolution sexual reproduction between MHC-divergent (i.e. negative assortative mating, NAM) may be favoured. However, strongest evidence for MHC-based NAM comes from inbred animals, and context-dependent mating preferences rarely been evaluated although they often occur...
The population genetic structure of the estuarine crab, Scylla serrata (Forskål, 1775), was examined among shelf-connected locations and across a historical bio-geographic barrier. Over 300 individuals were sampled from multiple within coastal regions (western, northern eastern) Australia analysed for mutational differences at mitochondrial coding gene (COI). Analysis molecular variance indicated haplotypes to be structured regionally (P < 0.001), which contrasted with evidence panmixia...
Microsatellites and mitochondrial DNA (mtDNA) have traditionally been used in population genetics because of their variability presumed neutrality, whereas genes the major histocompatibility complex (MHC) are increasingly interest strong selective pressures shape standing variation. Despite potential for MHC genes, microsatellites, mtDNA sequences to complement one another deciphering history demography, three rarely tandem. Here we report on MHC, microsatellite, a single large eastern tiger...
The Immaculatus Group of Culicoides encompassing four species from Australia, New Caledonia, Fiji, Solomon Islands, Guinea and the Malay archipelago is revised. A diagnosis for group, descriptions males females C. shivasi sp. n. collessi n., a description male immaculatus Lee & Reye, redescription female agas Wirth Hubert together with keys their specific determination are pre-sented. Specific separation morphologically similar supported by DNA barcodes (mitochondrial cytochrome oxidase...
Inbreeding may lead to morphological malformations in a wide variety of taxa. We used genetic markers evaluate whether malformed urodeles were more inbred and/or had less diversity than normal salamanders. captured 687 adult and 1259 larval tiger salamanders ( Ambystoma tigrinum ), assessed each individual for gross malformations, surveyed variation among individuals using both cytoplasmic nuclear markers. The most common adults larvae brachydactyly, ectrodactyly polyphalangy. overall...
In this study, species boundaries were examined for 15 described and 2 undescribed within the economically important Culicoides subg. Avaritia Fox from Australasia Eastern Asia. We used an integrative taxonomic approach incorporating DNA barcoding, nuclear gene sequencing, retrospective morphological analyses. Some arbovirus vector such as fulvus Sen Das Gupta wadai Kitaoka genetically morphologically uniform across sampled distributions, but others including actoni Smith brevipalpis...
The monophyly of the Imicola complex, a natural species complex within subgenus C. subgen. Avaritia Fox biting midge genus Culicoides Latreille, is supported using morphological and molecular analyses. A diagnosis for group along with comparative redescriptions male female represented in Australasia, brevitarsis Kieffer nudipalpis Delfinado description asiatica Bellis sp. nov., are presented together keys their specific determination support status.
Abstract Among urodeles, ambystomatid salamanders are particularly amenable to genetic parentage analyses because they explosive aggregate breeders that typically have large progeny arrays. Such can lead direct inferences about otherwise cryptic aspects of salamander natural history, including the rate multiple mating, individual reproductive success, and spatial distribution clutches. In 2002, we collected eastern tiger ( Ambystoma tigrinum ) egg masses (> 1000 embryos) from a ~80 m...
Bluetongue virus (BTV) is a major pathogen of ruminants that transmitted by biting midges (Culicoides spp.). Australian BTV serotypes have origins in Asia and are distributed across the continent into two distinct episystems, one north another east. Culicoides brevitarsis vector Australia entire geographic range virus. Here, we describe isolation use DNA microsatellites gauge their ability to determine population genetic connectivity C. within with countries north. Eleven microsatellite...
Invasive grass weeds reduce farm productivity, threaten biodiversity, and increase weed control costs. Identification of invasive grasses from native has generally relied on the morphological examination floral material. DNA barcoding may provide an alternative means to identify co-occurring grasses, particularly during early growth stages when characters are unavailable for analysis. However, there no universal loci available barcoding. We herein evaluated utility six candidate (atpF...
The hemipteran suborder Auchenorrhyncha comprises a rich assemblage of plant feeding species, many which are widespread in distribution and act as vectors viral fungal diseases affecting plants.Species level identifications this group generally possible only by examination male specimens; prior DNA barcode analyses limited range indicate that approach may provide an expedient means to identify species within diverse group.In study we explored the utility barcoding for identification wider...
Light trap surveillance across northern Australia and Papua New Guinea (PNG) has detected the presence of several Oriental species Culicoides not previously reported from those countries which appear to have arrived in recent times. Detections C. nudipalpis Delfinado Western Australia, flavipunctatus Kitaoka palpifer Das Gupta Ghosh Northern Territory flavipunctatus, fulvus Sen orientalis Macfie Queensland (Qld) provide evidence multiple pathways for incursions biting midges into Australia....
Helicoverpa and Heliothis species include some of the world's most significant crop pests, causing billions dollars losses globally. As such, a number are regulated quarantine species. For agencies, crucial issue is distinguishing native from exotics, yet even this task often not feasible because poorly known local faunas difficulties identifying closely related species, especially immature stages. DNA barcoding scalable molecular diagnostic method that could provide solution to problem,...
Abstract The cue-lure-responding New Guinea fruit fly, Bactrocera trivialis , poses a biosecurity risk to neighbouring countries, e.g., Australia. In trapping programs, lure caught flies are usually morphologically discriminated from non-target species; however, DNA barcoding can be used confirm similar species where morphology is inconclusive, breviaculeus and B. rufofuscula . This take days—and laboratory—to resolve. A quicker, simpler, molecular diagnostic assay would facilitate more...
Abstract A morphological and molecular review of the genus Orosius Distant (Deltocephalinae: Opsiini) was undertaken using male genitalia DNA barcoding. We recognise 14 valid species genus, which two were indeterminate, based on females only recognised by barcodes. argentatus (Evans) stat. rev. is removed from synonymy with orientalis (Matsumura) after barcoding demonstrated that are distinct; small but consistent differences in also found to support hypothesis. The other species, genitalia,...
Abstract Recent focus on Culicoides species diversity in Thailand was prompted by a need to identify vectors responsible for the transmission of African Horse Sickness that country. To assist rapid genetic identification species, we sampled mitochondrial cytochrome c oxidase subunit I (COI) DNA barcodes (N = 78) from 40 biting midge Thailand, including 17 which were previously unavailable. The assigned 39 Barcode Identification Numbers (BINs) representing terminal clusters at Life Data...
Mud crabs (Portunidae; Scylla spp.) have become established recently in some south-west Australian estuaries – almost 1000 km south of their recorded distribution. Colonisation may occurred by a natural range expansion from the north-west or translocation source(s) within Indo-West Pacific. To identify species and potential source population(s), genetic analyses was used to compare (N = 32) other populations. Levels diversity at two independent markers were also compared obtain relative...