A5011945886- Immunotherapy and Immune Responses
- Histone Deacetylase Inhibitors Research
- CAR-T cell therapy research
- Cancer Cells and Metastasis
- Skin and Cellular Biology Research
- Renal cell carcinoma treatment
- Cancer Immunotherapy and Biomarkers
- Bladder and Urothelial Cancer Treatments
- Pancreatic and Hepatic Oncology Research
- Cervical Cancer and HPV Research
- Head and Neck Cancer Studies
- Single-cell and spatial transcriptomics
- Galectins and Cancer Biology
- Cellular Mechanics and Interactions
- Nonmelanoma Skin Cancer Studies
- Fibroblast Growth Factor Research
- Skin Protection and Aging
- Sirtuins and Resveratrol in Medicine
- Urinary and Genital Oncology Studies
- Hearing, Cochlea, Tinnitus, Genetics
- Calcium signaling and nucleotide metabolism
- Cancer Research and Treatments
- Esophageal Cancer Research and Treatment
- Thyroid Cancer Diagnosis and Treatment
- Polyamine Metabolism and Applications
University of Wisconsin–Madison
2021-2025
University of Wisconsin Carbone Cancer Center
2022-2024
University of Ljubljana
2019-2024
Institute of Oncology Ljubljana
2021
Abstract Purpose: We investigated whether in human head and neck squamous cell carcinoma (HNSCC) high levels of expression stress keratin 17 (K17) are associated with poor survival resistance to immunotherapy. Experimental Design: the role K17 regulating both tumor microenvironment immune responsiveness HNSCC using a syngeneic mouse model, MOC2. MOC2 gives rise immunologically cold tumors that resistant immune-checkpoint blockade (ICB). engineered multiple, independent knockout (KO) lines...
Abstract Vulvar squamous cell carcinoma (VSCC) is a rare gynecological cancer with projection of 35% increase in the next 20 years North America. HPV known risk factor VSCC, but HPV+ and HPV- subtypes VSCC have previously been reported to be biologically not clinically distinct. In addition, could categorized by TP53 status different prognoses. However, tumor microenvironment (TME) its contributions patient clinical outcomes are well understood. Here, we use single-cell spatial omics...
Circulating tumor cell count is an independent prognostic factor in early breast cancer. CTCs can be found the blood of 20% patients prior to neoadjuvant therapy. We aimed assess suitability magnetic-activated separation (MACS) technology for isolation and cytological characterization CTCs. In preclinical part study, lines were spiked into buffy coat samples derived from healthy donors, isolated using MACS. Breast cancer cells with preserved morphology successfully isolated. clinical part,...
Predicting which patients will progress to metastatic disease after surgery for non-metastatic clear cell renal carcinoma (ccRCC) is difficult; however, recent data suggest that tumor immune infiltration could be used as a biomarker. We evaluated the quantity and type of cells infiltrating ccRCC tumors associations with progression following attempted curative surgery. quantified densities in microenvironment validated our findings two independent patient cohorts multi-region sampling...
Background: Approximately 15-20% of head and neck cancer squamous cell carcinoma (HNSCC) patients respond favorably to immune checkpoint blockade (ICB). Previous single-cell RNA-Seq (scRNA-Seq) studies identified features, including macrophage subset ratios T-cell subtypes, in HNSCC ICB response. However, the spatial features HNSCC-infiltrated cells response treatment need be better characterized. Methods: Here, we perform a systematic evaluation interactions between types within tumor...
Resveratrol (3,4,5-trihydroxystilbene) is a naturally occurring phytoalexin with purported health-promoting effects, but limited oral bioavailability. Our prior murine modeling research observed enhanced resveratrol bioavailability piperine co-administration. In this study, single-dose pharmacokinetics of or without and the associated toxicities were studied on cohort healthy volunteers. We performed double-blind, randomized, three-arm pilot study. Participants randomized to receive single...
We performed a clinical trial in patients with non-muscle-invasive (NMI) urothelial cancer randomized (2:1) to the EGFR tyrosine kinase inhibitor erlotinib or placebo (either orally once weekly × 3 doses prior scheduled surgery) assess for difference phosphorylation tumor-adjacent normal urothelium <24 hours post-study dose and tolerance of therapy. Thirty-seven volunteers (6 female/31 male; mean age 70; 35 White/2 non-White) confirmed suspected NMI were enrolled into either (n = 24; 900...
Approximately 40% of vulvar squamous cell carcinoma (vSCC) cases are etiologically associated with high-risk human papillomaviruses (HPVs) the alpha genera (α-HPV) that cause other anogenital cancers; however, etiology α-HPV-negative vSCC is poorly understood. HPVs beta (β-HPV) risk factors for cutaneous (cSCC) and may be related to carcinomas originating in sites such as vulva. In this study, we investigate presence β-HPVs, an emphasis on p16-negative lesions adjacent vSCC. We subjected 28...
Low response rates in immune check-point blockade (ICB)-treated head and neck squamous cell carcinoma (HNSCC) drive a critical need for robust, clinically validated predictive biomarkers. Our group previously showed that stress keratin 17 (CK17) suppresses macrophage-mediated CXCL9/CXCL10 chemokine signaling involved attracting activated CD8+ T cells into tumors, correlating with decreased rate to pembrolizumab-based therapy pilot cohort of ICB-treated HNSCC (n = 26). Here, we performed an...
Objective Evaluate the effects of α‐difluoromethylornithine (DFMO) on hearing thresholds as part a randomized, double‐blind, placebo‐controlled trial. Methods Subjects were randomized and assigned to control (placebo) or experimental group. DFMO placebo administered orally (500 mg/m 2 /day) for up 5 years. Results taking had, average, increased from baseline across frequency range compared subjects in Statistical analysis revealed this was significant lower range. Conclusions This controlled...
Risk-reducing mastectomy (RRM) is one of key prevention strategies in female carriers germline BRCA pathogenic/likely pathogenic variants (PV/LPV). We retrospectively investigated the rate, timing and longitudinal trends bilateral RRM uptake incidence types cancers among unaffected who underwent genetic counseling at Institute Oncology Ljubljana Slovenia.Female without personal history cancer were included study. Clinical data on PV/LPV type, date RRM, type reconstructive procedure, occult...
Background Here we report clinical risk factors and event rates for the development of new non-melanoma skin cancer (NMSC) in a randomized, double-blind, placebo-controlled trial irreversible ornithine decarboxylase (ODC) inhibitor, difluromethylornithine (DFMO), over 3–5-year follow-up. Methods 147 placebo patients (white; mean age 60.2 years; 60% male) were evaluated association initial biomarkers baseline patient characteristics with squamous cell (SCC) basal (BCC) carcinomas. Results...
<h3>Background</h3> Like many cancer types, only ~20% of head and neck (HNC) patients respond favorably to immune checkpoint blockade (ICB) therapy. Previous single-cell RNA-Seq (scRNA-Seq) studies identified features, including macrophage subset ratios T-cell subtypes,<sup>1–3</sup> in HNC ICB outcomes. However, the spatial features HNC-infiltrated cells response treatment need be better characterized. Here we perform a systematic evaluation cell interactions between types within tumor...
Supplementary Figure from Stress Keratin 17 Expression in Head and Neck Cancer Contributes to Immune Evasion Resistance Immune-Checkpoint Blockade
Supplementary Figure from Stress Keratin 17 Expression in Head and Neck Cancer Contributes to Immune Evasion Resistance Immune-Checkpoint Blockade
Supplementary Data from Stress Keratin 17 Expression in Head and Neck Cancer Contributes to Immune Evasion Resistance Immune-Checkpoint Blockade