A5070991823- Esophageal Cancer Research and Treatment
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Peptidase Inhibition and Analysis
- Microfluidic and Bio-sensing Technologies
- Cancer Immunotherapy and Biomarkers
- Lung Cancer Treatments and Mutations
- Fish Biology and Ecology Studies
- Fish Ecology and Management Studies
- Gastrointestinal Tumor Research and Treatment
- Phosphodiesterase function and regulation
- Mathematical Biology Tumor Growth
- Chemical Reactions and Isotopes
- Pancreatic and Hepatic Oncology Research
- Gastric Cancer Management and Outcomes
- Immune cells in cancer
- RNA modifications and cancer
- Cell Image Analysis Techniques
- Tea Polyphenols and Effects
- Genetics and Neurodevelopmental Disorders
- Healthcare professionals’ stress and burnout
- Electrohydrodynamics and Fluid Dynamics
- Phytoestrogen effects and research
University of Southampton
2018-2024
University Hospital Southampton NHS Foundation Trust
2022
Southampton General Hospital
2020-2021
University of Limerick
2020
Faculty (United Kingdom)
2019
University of Bristol
2018
South Texas Accelerated Research Therapeutics
2018
Sewanee: The University of the South
2018
Flinders University
2017
Michigan Medicine
2014
Hybridization between introduced and indigenous species can lead to loss of unique genetic resources precipitate extinction. In Tanzania, the Nile tilapia (Oreochromis niloticus) blue-spotted leucostictus) have been widely non-native habitats for aquaculture development capture fisheries. Here, we aimed quantify interspecific hybridization these Oreochromis esculentus, jipe korogwe. Pangani basin, several hybrids were observed (O. niloticus × O. jipe, leucostictus korogwe), although...
Among the many negative impacts of invasive species, hybridization with indigenous species has increasingly become recognized as a major issue. However, relatively few studies have characterized phenotypic outcomes following biological invasions. Here we investigate genetic and morphological consequences stocking tilapia in two water bodies central Tanzania. We sampled individuals from Mindu Reservoir on Ruvu river system, at Kidatu Great Ruaha-Rufiji system. screened 16 microsatellite loci,...
Tea provides health benefits, while oxidation is part of tea processing. The effect on the antithrombotic properties lipid extracts was evaluated for first time. Total lipids (TL) extracted from fresh leaves and commercial powder, before after 30-60 min oxidation, were further fractionated into neutral (NL) polar (PL). bioactivities TL, PL, NL assessed in human platelets against inflammatory mediator platelet-activating factor. PL thrombin, collagen, adenosine diphosphate, their fatty acid...
The tumor microenvironment (TME) comprises all non-tumor elements of cancer and strongly influences disease progression phenotype. To understand biology accurately test new therapeutic strategies, representative models should contain both cells normal the TME. Here we describe characterize co-culture tumor-derived organoids cancer-associated fibroblasts (CAFs), a major component TME, in matrix-embedded assembloid esophageal adenocarcinoma (EAC). We demonstrate that faithfully recapitulate...
Particles in a rotating fluid experience gravity-directed orbits to remain suspension.
Pirouette coupling involves Dean flow for cell and reporter bead inertial ordering efficient co-encapsulation, achieving a throughput of 1 million cells per hour, 2.5% multiplet rate 70% capture efficiency.
The tumor microenvironment (TME) comprises all non-tumor elements of cancer and strongly influences disease progression phenotype. To understand biology accurately test new therapeutic strategies, representative models should contain both cells normal the TME. Here, we describe characterize co-culture tumor-derived organoids cancer-associated fibroblasts (CAFs), a major component TME, in matrix-embedded assembloid esophageal adenocarcinoma (EAC). We demonstrate that faithfully recapitulate...
This tool is a unique comprehensive scale and methodology to measure the psychosocial acuity of patients families across health care continuum. Coupled with other measures, can tell complete compelling story social work contributions aid in resource alignment. Accurately conveying full scope value anyone, especially system leadership, requires that patient family be measured factored into equation, along productivity, time spent, services provided. The development utilization Psychosocial...
Single-nuclei RNA sequencing allows single cell-based analysis in frozen tissue, ameliorating cell recovery biases associated with enzymatic dissociation methods. The authors present two optimized methods for isolating and nuclei from esophageal tissue using a commercial EZ citric acid (CA)-based method. Despite high endogenous RNase activity, these protocols produced libraries of expected fragment length (average EZ: 745 bp; CA: 1232 bp) comparable complexity (median Transcript/Gene number,...
Abstract Background: Esophageal adenocarcinoma (EAC) is usually resistant to cytotoxic therapies and immunotherapies have gained little traction. Cancer-associated fibroblasts (CAF) are a major stromal cell population in the EAC tumor microenvironment associated with prognosis treatment outcomes. Recent evidence suggests that cancer phenotypes related EMT may determine CAF heterogeneity, but molecular cellular biology underlies myofibroblast fate not well understood. Methods: To obtain most...
Abstract The tumor microenvironment (TME) comprises all non-tumor elements of cancer and strongly influences disease progression phenotype. To understand biology accurately test new therapeutic strategies, representative models should contain both cells normal the TME. Here we describe characterize co-culture tumor-derived organoids cancer-associated fibroblasts (CAFs), a major component TME, in matrix-embedded assembloid esophageal adenocarcinoma (EAC). We demonstrate that faithfully...
Abstract Background The development of novel treatments for oesophageal adenocarcinoma (OAC) represents an area significant unmet need. Despite the application immune checkpoint blockade alongside chemotherapy as standard care some patients with metastatic OAC, responses to immunotherapies are modest and 5-year survival is 17%. reasons this remains unclear while tumour infiltration CD8+ T lymphocytes (TILs) at time resection associated improved outcomes, heterogeneous population incompletely...
Abstract Background and aims Esophageal adenocarcinoma (EAC) is chemoresistant in the majority of cases. The tumor-promoting biology cancer associated fibroblasts (CAF) make them a target for novel therapies. Phosphodiesterase type 5 inhibitors (PDE5i) have been shown to regulate activated fibroblast phenotype benign disease. We investigated potential CAF modulation EAC using PDE5i enhance efficacy chemotherapy. Methods were treated with phenotypic effects examined immunoblotting,...
Abstract In esophageal adenocarcinoma (EAC) with apparent pathological complete response (pCR) to neoadjuvant therapy (NAT) debate remains as whether esophagectomy is required. Recurrence after pCR not limited distant metastases outside radiation or resection fields. It unknown if cancer persists below the lower detection limit of current diagnostic technology. Trials randomising patients active surveillance are currently recruiting in an attempt spare morbidity esophagectomy. Methods Single...
A protocol to dissociate fresh esophagus tissue specimens for single-cell transcriptomics.
A protocol to dissociate frozen esophagus tissue specimens for single-nuclei transcriptomics.
Abstract Background Esophageal adenocarcinoma (EAC) develops in a complex ecosystem that defines tumour evolution, response to treatment and patient outcomes. We have shown high levels of cancer associated fibroblast (CAF) the microenvironment (TME) predicts poor outcome is inversely related infiltrating lymphocyte (TILs) abundance. Bulk sequencing studies lack resolution dissect phenotypic functional heterogeneity cell-cell interactions TME. applied single cell RNA 12 EAC patients address...