A5035187448- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- 3D Printing in Biomedical Research
- Mesenchymal stem cell research
- Drug-Induced Hepatotoxicity and Protection
- Tissue Engineering and Regenerative Medicine
- Animal testing and alternatives
- Computational Drug Discovery Methods
- Metabolomics and Mass Spectrometry Studies
- Pancreatic function and diabetes
- Pluripotent Stem Cells Research
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Endoplasmic Reticulum Stress and Disease
- Drug Transport and Resistance Mechanisms
- Pharmacogenetics and Drug Metabolism
- Hepatitis C virus research
- Cardiovascular Disease and Adiposity
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Biochemical effects in animals
- Lipid metabolism and disorders
- Alcohol Consumption and Health Effects
- Effects and risks of endocrine disrupting chemicals
- Liver Disease and Transplantation
Vrije Universiteit Brussel
2016-2025
National Institute on Alcohol Abuse and Alcoholism
2019-2022
National Institutes of Health
2019-2022
Pharmac
2013-2021
Institute for In Vitro Sciences
2021
Joint Research Centre
2011-2012
Scott & White Memorial Hospital
2009
Texas A&M University
2009
Goethe University Frankfurt
2009
Neutrophil infiltration around lipotoxic hepatocytes is a hallmark of nonalcoholic steatohepatitis (NASH); however, how these 2 types cells communicate remains obscure. We have previously demonstrated that neutrophil-specific microRNA-223 (miR-223) elevated in to limit NASH progression obese mice. Here, we this elevation miR-223 was due preferential uptake miR-223–enriched extracellular vesicles (EVs) derived from neutrophils as well other cells, albeit lesser extent. This selective...
This study employs animal-free Next Generation Risk Assessment (NGRA) principles to evaluate the safety of repeated dermal exposure 2.5% (w/w) HC Yellow No. 13 (HCY13) hair dye. As multiple in silico tools consistently flagged hepatotoxic potential, likely due HCY13's trifluoromethyl group, which is known interfere with hepatic lipid metabolism, liver steatosis was chosen as primary mode action for evaluation. AOP-guided vitro tests were conducted, exposing human stem cell-derived cells...
Background and Aims Hepatic cardiomyopathy, a special type of heart failure, develops in up to 50% patients with cirrhosis is major determinant survival. However, there no reliable model hepatic cardiomyopathy mice. We aimed characterize the detailed hemodynamics mice bile duct ligation (BDL)–induced liver fibrosis, by monitoring echocardiography intracardiac pressure–volume relationships myocardial structural alterations. Treatment selective cannabinoid‐2 receptor (CB 2 ‐R) agonist, known...
Background and Aims Neutrophil infiltration is a hallmark of nonalcoholic steatohepatitis (NASH), but how this occurs during the progression from steatosis to NASH remains obscure. Human features hepatic neutrophil up‐regulation major neutrophil‐recruiting chemokines (e.g., chemokine [C‐X‐C motif] ligand 1 [CXCL1] interleukin [IL]‐8). However, mice fed high‐fat diet (HFD) only develop fatty liver without significant or elevation chemokines. The aim study was determine why are resistant...
Alcohol-associated liver disease is a spectrum of disorders with histopathological changes ranging from simple steatosis to steatohepatitis, cirrhosis, and hepatocellular carcinoma. Recent data suggest that chronic-plus-binge ethanol intake induces steatohepatitis by promoting release hepatocytes proinflammatory mitochondrial DNA–enriched (mtDNA-enriched) extracellular vesicles (EVs). The aim the present study was investigate role stress kinase apoptosis signal–regulating 1 (ASK1) p38...
Human adult stem cells (hASCs) have become an attractive source for autologous cell transplantation, tissue engineering, developmental biology, and the generation of human-based alternative in vitro models. Among 3 germ layers, mesoderm is origin today's most widely used characterized hASC populations. A variety isolated nonhematopoietic mesoderm-derived populations exist, all them show important differences terms function, efficacy, differentiation potential both vivo vitro. To better...
Human skin-derived precursors (hSKP) are postnatal stem cells with neural crest properties that reside in the dermis of human skin. These can be easily isolated from small (fore) skin segments and have capacity to differentiate into multiple cell types. In this study, we show upon exposure hepatogenic growth factors cytokines, hSKP acquire sufficient hepatic features could make these suitable vitro tools for hepatotoxicity screening new chemical entities already existing pharmaceutical...
Nonalcoholic steatohepatitis (NASH) is a leading cause of chronic liver disease, characterized by steatosis and hallmark neutrophil infiltration. NASH also associated with adipose tissue inflammation, but the role inflammation in pathogenesis remains obscure. The aim this study was to investigate interplay between recruitment progression NASH.A mouse model obtained high-fat diet (HFD) feeding plus adenovirus-Cxcl1 overexpression (HFD+AdCxcl1). Genetic deletion E-selectin (Sele) treatment an...
While gap junctions mediate intercellular communication and support liver homeostasis, connexin hemichannels are preferentially opened by pathological stimuli, including inflammation oxidative stress. The latter essential features of non-alcoholic steatohepatitis. In this study, it was investigated whether connexin32 connexin43 play a role in Mice were fed choline-deficient high-fat diet or normal for 8 weeks. Thereafter, TAT-Gap24 TAT-Gap19, specific inhibitors composed connexin43,...