A5004117957- Epigenetics and DNA Methylation
- Nutrition, Genetics, and Disease
- Phytoestrogen effects and research
- BRCA gene mutations in cancer
- Cancer-related gene regulation
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Sirtuins and Resveratrol in Medicine
- Prostate Cancer Treatment and Research
- Cancer, Lipids, and Metabolism
- Histone Deacetylase Inhibitors Research
- Digestive system and related health
- Genetic factors in colorectal cancer
- Antioxidant Activity and Oxidative Stress
- RNA modifications and cancer
- Fatty Acid Research and Health
- Genomics and Chromatin Dynamics
- Cancer-related Molecular Pathways
- Cancer Risks and Factors
- Molecular Biology Techniques and Applications
- Retinoids in leukemia and cellular processes
- Ubiquitin and proteasome pathways
- Nutritional Studies and Diet
- Genomic variations and chromosomal abnormalities
- RNA and protein synthesis mechanisms
Centre Jean Perrin
2013-2022
Imagerie Moléculaire et Stratégies Théranostiques
2018-2022
Inserm
1999-2021
Université Clermont Auvergne
1999-2018
Genetique Reproduction and Developpement
2015
Foie, Métabolisme, Cancer
2010-2012
Centre de Recherche en Nutrition Humaine d'Auvergne
2007-2012
H3K27me3 histone marks shape the inhibition of gene transcription. In prostate cancer, deregulation might play a role in tumor progression.We investigated genome-wide methylation profile using chromatin immunoprecipitation (ChIP) and 2X400K promoter microarrays to identify differentially-enriched regions biopsy samples from cancer patients. were assessed 34 tumors: 11 with Gleason score > 7 (GS 7), 10 ≤ 13 morphologically normal samples.Here, profiling identified an average 386...
The isoflavones genistein, daidzein and equol (daidzein metabolite) have been reported to interact with epigenetic modifications, specifically hypermethylation of tumor suppressor genes. objective this study was analyze understand the mechanisms by which phytoestrogens act on chromatin in breast cancer cell lines.Two lines, MCF-7 MDA-MB 231, were treated genistein (18.5 µM), (78.5 (12.8 17β-estradiol (10 nM) suberoylanilide hydroxamic acid (1 µM) for 48 h. A control untreated cells...
Although soy phytoestrogens have been postulated to exert a protective effect against breast cancer, the attendant mechanisms, in particular epigenetics underpinnings, remained elusive. We investigated putative effects on DNA methylation by two naturally occurring isoflavones, genistein and daidzein, study of BRCA1 BRCA2 oncosuppressor genes cancer cell lines (MCF-7, MDA-MB 231, MCF10a). A demethylant agent, 5-azacytidine, methylant, budesonide, were used as treatment controls. was with...
Aim: DNA hypermethylation is an epigenetic mechanism which induces silencing of tumor-suppressor genes in prostate cancer. Many studies have reported that specific components food plants like soy phytoestrogens may protective effects against carcinogenesis or progression. Genistein and daidzein, the major phytoestrogens, been to ability reverse cancer cell lines. The aim this study was investigate potential demethylating these two compounds on BRCA1, GSTP1, EPHB2 BRCA2 promoter genes....
Breast cancer is the most frequently diagnosed malignancy in women worldwide. It well established that complexity of carcinogenesis involves profound epigenetic deregulations contribute to tumorigenesis process. Deregulated H3 and H4 acetylated histone marks are amongst those alterations. Sirtuin-1 (SIRT1) a class-III deacetylase deeply involved apoptosis, genomic stability, gene expression regulation breast tumorigenesis. However, underlying molecular mechanism by which SIRT1 regulates...
Obesity is a risk factor for breast cancer in postmenopausal women. Leptin, hormone excessively produced during obesity, suggested to be involved cancer. The aim of the study was investigate procarcinogenic potential leptin by evaluating influence on cell proliferation, cycle, apoptosis, and signaling numerous cells lines, including 184B5 normal cells, MCF10A fibrocystic MCF-7, MDA-MB-231, T47D cells. Expressions Ob-R were analyzed using qRT-PCR immunohistochemistry, proliferation...
It is well established that genetic and epigenetic alterations are common events in prostate cancer, which may lead to aberrant expression of critical genes. The importance mechanisms cancer carcinogenesis increasingly evident. In this study, the focus will be on histone modifications primary objectives map H3K27me3 marks quantify RAR beta 2, ER alpha, SRC3, RGMA, PGR, EZH2 gene expressions tissues compared normal tissues. addition, a data analysis was made connection with...
Among the micronutrients studied in relation between nutrition and cancer, lycopene appears to be a breast cancer preventive phytochemical candidate found raw tomatoes tomato-derived products. In order investigate responsiveness of genes better understand molecular mechanisms underlying effects lycopene, we used an oligonucleotide microarray approach. Human cell lines (MCF-7 MDA-MB-231) fibrocystic line (MCF-10a) were either exposed or not 10 µM for 48 h. Microarrays comprising 202 identify...