A5021286838- Microbial Community Ecology and Physiology
- Anaerobic Digestion and Biogas Production
- Wastewater Treatment and Nitrogen Removal
- Genomics and Phylogenetic Studies
- Methane Hydrates and Related Phenomena
- Microbial Fuel Cells and Bioremediation
- Gut microbiota and health
- Composting and Vermicomposting Techniques
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Pharmaceutical and Antibiotic Environmental Impacts
- Microplastics and Plastic Pollution
- Constructed Wetlands for Wastewater Treatment
- Protist diversity and phylogeny
- Legume Nitrogen Fixing Symbiosis
- Plant Disease Resistance and Genetics
- Microbial metabolism and enzyme function
- Nematode management and characterization studies
- Soil Carbon and Nitrogen Dynamics
- Water Treatment and Disinfection
- biodegradable polymer synthesis and properties
- Microbial bioremediation and biosurfactants
- Probiotics and Fermented Foods
- Plant Pathogens and Fungal Diseases
- Fecal contamination and water quality
National Institute of Advanced Industrial Science and Technology
2016-2025
Hokkaido University
2024-2025
Nagaoka University of Technology
2024
University of Illinois Urbana-Champaign
2013-2020
Toyohashi University of Technology
2000-2016
Japan Patent Office
2011
Anaerobic digestion (AD) processes are known to effectively convert organic waste CO2 and CH4 , but much of the microbial ecology remains unclear. Specifically, we have limited insights into symbiotic syntroph methanogen ('syntrophy') acid degradation, although they essential for preventing process deterioration. Also, often observed many uncharacterized or uncultivated organisms, poorly understood their role(s) in relation syntrophy. To define syntrophy-associated populations, this study...
Reduction of crystalline Fe(III) oxides is one the most important electron sinks for organic compound oxidation in natural environments. Yet limited number isolates makes it difficult to understand physiology and ecological impact microorganisms involved. Here, two-stage cultivation was implemented selectively enrich isolate iron(III) oxide reducing soils sediments. Firstly, iron reducers were enriched other untargeted eutrophs depleted by 2-years successive culture on a ferric (i.e.,...
Anaerobic digestion is a common technology to biologically stabilize wasted solids produced in municipal wastewater treatment. Its efficiency usually evaluated by calculating the reduction volatile solids, which assumes no biomass growth associated with digestion. To determine whether this assumption valid and further evaluate efficiency, study sampled 35 digester sludge from different reactors at multiple time points together feed full-scale water reclamation plant Chicago, Illinois. The...
Current understanding of amino acid (AA) degraders in anaerobic digesters is mainly based on cultured species, whereas microorganisms that play important roles a complex microbial community remain poorly characterized. This study investigated short-term enrichments degrading single AAs using metagenomics and metatranscriptomics. Metagenomic analysis revealed populations related to AA had an abundance <2.5% the sequences. In contrast, metagenomic-assembled bins uncultured Bacteroidales...
Although the anaerobic ammonium oxidation (anammox) process has attracted attention regarding its application in ammonia wastewater treatment based on efficiency, physiological characteristics of anammox bacteria remain unclear because lack pure-culture representatives. The coexistence heterotrophic often been observed reactors, even those fed with synthetic inorganic nutrient medium. In this study, we recovered 37 draft genome bins from a long-term-operated column reactor and predicted...
Abstract Background Current understanding of the carbon cycle in methanogenic environments involves trophic interactions such as interspecies H 2 transfer between organotrophs and methanogens. However, many metabolic processes are thermodynamically sensitive to accumulation can be inhibited by produced from co-occurring metabolisms. Strategies for driving competing metabolisms remain unexplored. Results To uncover how anaerobes combat this conflict situ, we employ metagenomics...
One highly diverse phylogenetic group of Bacteria, Ca . Patescibacteria, remains poorly understood, but, from the few cultured representatives and metagenomic investigations, they are thought to live symbiotically or parasitically with other bacteria even eukarya.
Four obligately anaerobic, thermophilic, sulfate-reducing bacterial strains, designated TGE-P1(T), TDV(T), TGL-LS1 and TSL-P1, were isolated from thermophilic (operated at 55 degrees C) methanogenic sludges waste wastewater treatment. The optimum temperature for growth of all the strains was in range 55-60 C. four grew by reduction sulfate with a limited electron donors, such as hydrogen, formate, pyruvate lactate. In co-culture hydrogenotrophic methanogen Methanothermobacter...
A comprehensive survey of bacterial and archaeal community structures within granular sludges taken from twelve different types full-scale, food-processing wastewater-treating, upflow anaerobic sludge blanket (UASB) reactors was performed with a 16S rRNA gene-based clone library method. In total, 1,282 gene clones 722 were analyzed, their identities determined by phylogenetic analyses. Overall, belonging to the phyla Proteobacteria (the class Deltaproteobacteria in particular), Firmicutes,...
How aromatic compounds are degraded in various anaerobic ecosystems (e.g. groundwater, sediments, soils and wastewater) is currently poorly understood. Under methanogenic conditions (i.e. groundwater wastewater treatment), syntrophic metabolizers known to play an important role. This study explored the draft genome of Syntrophorhabdus aromaticivorans strain UI identified first phenol-degrading phenylphosphate synthase (PpsAB) carboxylase (PpcABCD) terephthalate-degrading decarboxylase...
ABSTRACT Despite their importance as a biofuel production platform, only very limited number of butanol-tolerant bacteria have been identified thus far. Here, we extensively explored butanol- and isobutanol-tolerant from various environmental samples. A total 16 aerobic anaerobic that could tolerate greater than 2.0% (vol/vol) butanol isobutanol were isolated. 16S rRNA gene sequencing analysis revealed the isolates phylogenetically distributed over at least nine genera: Bacillus ,...
Patescibacteria are widely distributed in various environments and often detected activated sludge. However, limited information is currently available on their phylogeny, morphology, ecophysiological role sludge or interactions with other microorganisms. In the present study, we identified microorganisms that interacted via a correlation ana-lysis using 16S rRNA gene, predicted metabolic potential of metagenomic ana-lysis. The metagenome-assembled genomes consisted three Saccharimonadia,...
Deciphering unclear microbial interactions is key to improving biological wastewater treatment processes. Microbial predation and parasitism in ecosystems are unexplored survival strategies that have long been known recently attracted attention because these interspecies may contribute the reduction of excess sludge. Here, community profiling 600 activated sludge samples taken from six industrial one municipal processes (WWTPs) was conducted. To identify shared lineages WWTPs, constituents...
To verify whether members of the phylum