A5052612191- Wastewater Treatment and Nitrogen Removal
- Microbial Fuel Cells and Bioremediation
- Microbial Community Ecology and Physiology
- Water Treatment and Disinfection
- Microbial Metabolic Engineering and Bioproduction
- Anaerobic Digestion and Biogas Production
- Geochemistry and Elemental Analysis
- Reproductive Biology and Fertility
- Radioactive element chemistry and processing
- Biofuel production and bioconversion
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Carbon Dioxide Capture Technologies
- Marine Sponges and Natural Products
- Algal biology and biofuel production
- Ammonia Synthesis and Nitrogen Reduction
- Constructed Wetlands for Wastewater Treatment
- Membrane Separation Technologies
- Assisted Reproductive Technology and Twin Pregnancy
- Glycosylation and Glycoproteins Research
- Probiotics and Fermented Foods
- Industrial Gas Emission Control
- Protist diversity and phylogeny
- Ovarian function and disorders
- Bacterial biofilms and quorum sensing
Hiroshima University
2015-2024
Northeastern University
2013-2016
Waseda University
2000-2014
Akebono Clinic
2008-2013
Hokkaido University of Education
2000
Aoyama Gakuin University
1995
The University of Tokyo
1976
Although the anaerobic ammonium oxidation (anammox) process has attracted attention regarding its application in ammonia wastewater treatment based on efficiency, physiological characteristics of anammox bacteria remain unclear because lack pure-culture representatives. The coexistence heterotrophic often been observed reactors, even those fed with synthetic inorganic nutrient medium. In this study, we recovered 37 draft genome bins from a long-term-operated column reactor and predicted...
Abstract Background Isopropanol (IPA) is a commodity chemical used as solvent or raw material for polymeric products, such plastics. Currently, IPA production depends largely on high-CO 2 -emission petrochemical methods that are not sustainable. Therefore, alternative low-CO emission required. bioproduction using biomass waste gas promising method. Results Moorella thermoacetica , thermophilic acetogenic microorganism, was genetically engineered to produce IPA. A metabolic pathway related...
ABSTRACT A hollow-fiber membrane chamber (HFMC) was developed as an in situ cultivation device for environmental microorganisms. The HFMC system consists of 48 to 96 pieces porous connected with injectors. allows rapid exchange chemical compounds, thereby simulating a natural environment. Comparative analysis through the three types samples performed using this newly designed and conventional agar-based petri dish. results show that ratios novel phylotypes isolates, species-level...
Summary This study evaluates the community structure in nitrifying granules (average diameter of 1600 μm) produced an aerobic reactor fed with ammonia as sole energy source by a multivalent approach combining molecular techniques, microelectrode measurements and mathematical modelling. Fluorescence situ hybridization revealed that ammonia‐oxidizing bacteria dominated within first 200 μm below granule surface, nitrite‐oxidizing deeper layer between 300 μm, while heterotrophic were present...
Nitrite oxidation is a key step in nitrogen removal biological wastewater treatment plants. Recently, two phylogenetically different Nitrospira (sublineages I and II) have been recognized as the numerically dominant nitrite-oxidizing bacteria However, sublineage II inhabiting activated sludge was not isolated its detailed properties were unclear. In this study, we developed new method for isolation of forming micro-colonies using cell sorter. We obtained novel pure strain "Nitrospira...
Patescibacteria are widely distributed in various environments and often detected activated sludge. However, limited information is currently available on their phylogeny, morphology, ecophysiological role sludge or interactions with other microorganisms. In the present study, we identified microorganisms that interacted via a correlation ana-lysis using 16S rRNA gene, predicted metabolic potential of metagenomic ana-lysis. The metagenome-assembled genomes consisted three Saccharimonadia,...
Abstract Gas fermentation is one of the promising bioprocesses to convert CO 2 or syngas important chemicals. Thermophilic gas volatile chemicals has potential for development consolidated that can simultaneously separate products during fermentation. This study reports production acetone from and H , CO, by introducing pathway using acetyl–coenzyme A (Ac-CoA) acetate produced via Wood–Ljungdahl in Moorella thermoacetica . Reducing carbon flux Ac-CoA through genetic engineering successfully...
Nitrification is an important process in the biogeochemical nitrogen cycle and widely exploited biological wastewater treatment. Recently, Nitrospira has been recognized as numerically dominant nitrite-oxidizing bacterial genus primarily responsible for second step of aerobic nitrification. Nevertheless, physiological properties remain poorly understood because organisms are difficult to isolate culture. Here, we report a novel cultivation strategy obtaining members sublineage I pure The...
Until now, only few attempts have been made to assess biofilm models simulating microenvironments in a biofilm. As first step, we compare the microenvironment observed membrane aerated (MAB) that derived from two-dimensional computational model with individual ammonia oxidizing bacteria (AOB) and nitrite (NOB) embedded continuum EPS matrix. Gradients of oxygen were determined by means microelectrodes. The change nitrifying bacterial populations depth was quantified using fluorescence situ...
We present an automated method for isolating pure bacterial cultures from samples containing multiple species that exploits the cell's own physiology to perform separation. Cells compete reach a chamber nutrients via constriction whose cross-sectional area only permits single cell enter, thereby blocking opening and preventing other cells entering. The winning divides across its progeny populate chamber. devices are passive require no user interaction their function. Device fabrication...
Nitrification is an important step in nitrogen removal biological wastewater treatment processes. Recently, Nitrospira have been recognized as the numerically dominant nitrite-oxidizing bacterial genus primarily responsible for second of aerobic nitrification; however, usually resist cultivation under laboratory conditions and only one species enriched from activated sludge has described. In this study, a novel enrichment method was successfully developed using continuous feeding...
Candidatus Saccharibacteria is a well-described candidate phylum that has not been successfully isolated. Nevertheless, its presence was suggested by 16S rRNA gene sequencing data, and it frequently detected in natural environments activated sludge. Because pure culture representatives of are lacking, the specificity primers for determination their abundance diversity should be carefully evaluated. In this study, eight Saccharibacteria-specific were selected from previous studies evaluated...
To quantitatively analyse the changes to amoA mRNA (ammonia mono-oxygenase encoding mRNA) profiles in response a change ammonia oxidation activity complex microbial community.The levels both batch-mode incubation and continuously fed nitrification reactor were determined by real-time reverse transcription-PCR analysis. The level changed rapidly environmental conditions which affect activity.An increase can be detected within 1-2 h an initiation of cell whereas decrease is 24 cessation...
Standard cultivation fails to grow most microorganisms, whereas in situ allows for the isolation of comparatively diverse and novel microorganisms. Information on similarities differences physiological properties isolates obtained from standard is limited. Therefore, we used arctic sediment samples compared two culture collections using techniques. Even though there was no temperature selection at step, each method showed different reactions temperature. The results present study suggest...