A5113865332- Aquaculture Nutrition and Growth
- Crustacean biology and ecology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Invertebrate Immune Response Mechanisms
- Reproductive biology and impacts on aquatic species
- Neurobiology and Insect Physiology Research
- Physiological and biochemical adaptations
- Aquaculture disease management and microbiota
- Genetic diversity and population structure
- Animal Genetics and Reproduction
- Insect Resistance and Genetics
- Aquatic life and conservation
- Fish Biology and Ecology Studies
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Genetic and phenotypic traits in livestock
- Autophagy in Disease and Therapy
- High Altitude and Hypoxia
- Parasite Biology and Host Interactions
- Mosquito-borne diseases and control
- Identification and Quantification in Food
- Genomics and Phylogenetic Studies
- Marine Biology and Environmental Chemistry
- Environmental Toxicology and Ecotoxicology
- Metabolomics and Mass Spectrometry Studies
Ministry of Agriculture and Rural Affairs
2020-2025
Chinese Academy of Fishery Sciences
2016-2025
Nanjing Agricultural University
2016-2025
Shanghai Ocean University
2018-2023
Ministère de l'Agriculture et de la Souveraineté alimentaire
2017
University of Alberta
2013
Institute of Hydrobiology
2003
Chinese Academy of Sciences
2003
Institute of Molecular Biology, Academia Sinica
2002
Argonne National Laboratory
2002
Background The oriental river prawn, Macrobrachium nipponense, is an important aquaculture species in China, even whole of Asia. androgenic gland produces hormones that play crucial roles sexual differentiation to maleness. This study the first de novo M. nipponense transcriptome analysis using cDNA prepared from mRNA isolated gland. Illumina/Solexa was used for sequencing. Methodology and Principal Finding total volume RNA sample more than 5 ug. We generated 70,853,361 high quality reads...
Oriental river prawn, Macrobrachium nipponense, is a commercially important species found in brackish and fresh waters throughout China. Chronic hypoxia major physiological challenge for prawns culture, the hepatopancreas, muscle gill tissues play roles adaptive processes. However, effects of dissolved oxygen availability on gene expression functions those are unknown. Adaptation to complex process, help us understand stress-sensing mechanism ultimately permit selection hypoxia- tolerant...
Quantitative real-time PCR (qPCR) is widely used in molecular biology, although the accuracy of quantitative results determined by stability reference genes used. Recent studies have investigated suitable for some crustaceans under various conditions, but Macrobrachium nipponense are currently lacking. In this study, we selected following seven from among 35 commonly housekeeping as candidate qPCR temporal and spatial expression: EIF (eukaryotic translation initiation factor 5A), 18S (18S...
The oriental river prawn, Macrobrachium nipponense, is an economically important shrimp in China. Male prawns have higher commercial value than females because the former grow faster and reach larger sizes. It therefore to reveal sex-differentiation development mechanisms of prawn enable genetic improvement.We sequenced 293.3 Gb raw Illumina short reads 405.7 Pacific Biosciences long reads. final whole-genome assembly Oriental was ∼4.5 size, with predictions 44,086 protein-coding genes. A...
The oriental river prawn Macrobrachium nipponense is of great economic importance in China. However, its culture the saline–alkaline regions limited. This study used D-loop region sequences to analyze genetic diversity and population structure across A total 264 individuals were successfully sequenced from nine sites, including Daqing Heilongjiang Province (HLJ), Songyuan Jilin (JL), Ulanhot east inner Mongolia (NMG), Jingtai Gansu (GS), Dongying Shandong (SD), Dongtai Jiangsu (JS), Nanchang...
Abstract Background Macrobrachium nipponense is an economically important species of freshwater shrimp in China. Unlike other marine shrimps, the ovaries adult female M. can mature rapidly and periodically during reproductive period, but resulting high stocking densities environmental deterioration negatively impact harvest yield economic benefits. To better understand ovary development , we performed systematic transcriptome sequencing five different stages ovarian maturation. Results We...
In this study, two Sxl gene homologs, designated as Mnsxl1 and Mnsxl2, were cloned characterized from the freshwater prawn Macrobrachium nipponense by rapid amplification of cDNA ends.The deduced amino acid sequences Mnsxl2 showed high sequence homology to insect contained conserved domains in RNA-binding motifs.Real-time quantitative reverse transcription-polymerase chain reaction (RT-QPCR) that genes expressed all investigated tissues, with highest level expression intestine liver.RT-QPCR...
Abstract Background: The lined seahorse, Hippocampus erectus, is an Atlantic species and mainly inhabits shallow sea beds or coral reefs. It has become very popular in China for its wide use traditional Chinese medicine. In order to improve the aquaculture yield of this valuable fish species, we are trying develop genomic resources assistant selection genetic breeding. Here, provide whole genome sequencing, assembly, gene annotation which can enrich resource further application molecular...
Abstract The oriental river prawn, Macrobrachium nipponense , is an important commercial aquaculture resource in China. During breeding season, short ovary maturation cycles of female prawns cause multi-generation reunions ponds and affect the growth females representing individual miniaturization (known as autumn -propagation). These reproductive characteristics pose problems for large - scale farming. To date, molecular mechanisms reproduction regulation M . remain unclear. address this...
Hypoxia represents a major physiological challenge for prawns and is problem in aquaculture. Therefore, an understanding of the metabolic response mechanism economically important prawn species to hypoxia re-oxygenation essential. However, little known about intrinsic mechanisms by which oriental river Macrobrachium nipponense copes with at level. In this study, we conducted gas chromatography-mass spectrometry-based metabolomics studies assays energy metabolism-related parameters...
The doublesex and mab-3 related transcription factor (DMRT) gene family involvement in sex development is widely conserved from invertebrates to humans. In this study, we identified a DM (Doublesex/Mab-3)-domain Macrobrachium nipponense, which named MniDMRT11E because it has many similarities phylogenetically close relationships with the arthropod DMRT11E. Amino acid alignments structural prediction uncovered conservation putative active sites of domain. Real-time PCR analysis showed that...
White spot syndrome virus (WSSV) is one of the most devastating pathogens cultured shrimp, responsible for massive loss its commercial products worldwide. The oriental river prawn Macrobrachium nipponense an economically important species that widely farmed in China and adult prawns can be infected by WSSV. However, molecular mechanisms host pathogen interaction remain unknown. There urgent need to learn between M. WSSV which will able offer a solution controlling spread Next Generation...