A5048162063- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Viral Infections and Outbreaks Research
- Virology and Viral Diseases
- Reproductive System and Pregnancy
- COVID-19 epidemiological studies
- Viral gastroenteritis research and epidemiology
- Hepatitis B Virus Studies
- Bacillus and Francisella bacterial research
- Single-cell and spatial transcriptomics
- Viral Infections and Immunology Research
- Poxvirus research and outbreaks
- Animal Disease Management and Epidemiology
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- 3D Printing in Biomedical Research
- Herpesvirus Infections and Treatments
- Leptospirosis research and findings
- Endometriosis Research and Treatment
- Vector-borne infectious diseases
- Reproductive Physiology in Livestock
- T-cell and B-cell Immunology
Frederick National Laboratory for Cancer Research
2024
National Institutes of Health
2024
National Cancer Institute
2023-2024
Center for Cancer Research
2023-2024
United States Army Medical Research Institute of Infectious Diseases
2017-2021
Leidos Biomedical Research Inc. (United States)
2015
Leidos (United States)
2015
Unprotected sexual intercourse between persons residing in or traveling from regions with Zika virus transmission is a risk factor for infection. To model infection after intercourse, we inoculated rhesus and cynomolgus macaques by intravaginal intrarectal routes. In intravaginally, detected viremia RNA 50% of macaques, followed seroconversion. intrarectally, viremia, RNA, both, 100% both species, The magnitude duration infectious the blood suggest humans infected through will likely...
Concerns regarding outbreaks of human monkeypox or the potential reintroduction smallpox into an immunological naïve population have prompted development animal models and countermeasures. Here we present a marmoset model disease utilizing relevant poxvirus via natural exposure route. We found that 1000 plaque forming units (PFU) Monkeypox virus was sufficient to recapitulate disease, include incubation period approximately 13 days, followed by onset rash, death between 15 17 days....
Crimean-Congo hemorrhagic fever (CCHF) is the most medically important tick-borne viral disease of humans and tuberculosis leading cause death worldwide by a bacterial pathogen. These two diseases overlap geographically, however, concurrent infection CCHF virus (CCHFV) with mycobacterial has not been assessed nor ability to persist long-term sequela in primate model. In this study, we compared progression diverse strains CCHFV recently described cynomolgus macaque All animals demonstrated...
Recent Ebola virus (EBOV) outbreaks in West Africa and the Democratic Republic of Congo have highlighted urgent need for approval medical countermeasures treatment prevention EBOV disease (EVD). Until recently, when successes were achieved characterizing efficacy multiple experimental EVD therapeutics humans, only feasible way to obtain data regarding potential clinical benefits candidate was by conducting well-controlled animal studies. Nonclinical studies are likely continue be important...
To evaluate potential immunocompetent small animal models of Zika virus (ZIKV) infection, we inoculated Syrian golden hamsters (subcutaneously or intraperitoneally) and strain 13 guinea pigs (intraperitoneally) with Senegalese ZIKV ArD 41525 Philippines CPC-0740. We did not detect viremia in subcutaneously either strain, although some developed neutralizing antibodies. However, detected statistically significant higher viremias ( P = 0.0285) a median neutralization titer 0.0163)...
Introduction Interferon-gamma (IFN-γ) is pivotal in orchestrating immune responses during healthy pregnancy. However, its dysregulation, often due to autoimmunity, infections, or chronic inflammatory conditions, implicated adverse reproductive outcomes such as pregnancy failure infertility. Additionally, the underlying immunological mechanisms remain elusive. Methods Here, we explore impact of systemic IFN-γ elevation on cytotoxic T cell female reproduction utilizing a lupus-prone mouse...
Mosquito-borne and sexual transmission of Zika virus (ZIKV), a TORCH pathogen, recently initiated series large epidemics throughout the Tropics. Animal models are necessary to determine risk study pathogenesis, as well screen antivirals vaccine candidates. In this study, we modeled mosquito ZIKV in African green monkey (AGM). Following subcutaneous, intravaginal or intrarectal inoculation AGMs with ZIKV, determined potential infection dynamics virus. inoculated by all three routes exhibited...
Most alphaviruses are mosquito-borne and can cause severe disease in humans domesticated animals. In North America, eastern equine encephalitis virus (EEEV) is an important human pathogen with case fatality rates of 30-90%. Currently, there no therapeutics or vaccines to treat and/or prevent infection. One critical impediment countermeasure development the lack insight into clinically relevant parameters a susceptible animal model. This study examined course EEEV cynomolgus macaque model...
Here, we present a validated workflow to isolate sufficient viable single ovary cells from mouse without the need pool several mice. We provide steps essential for estrous staging, harvesting and dissociation, cell staining, data collection, analysis. Our approach allows use of these single-cell suspensions flow sorting, cytometry analysis, or functional in vitro assays. Importantly, our protocol is designed maximize isolation immune cells, including T subsets.
Ebola virus remains a significant public health concern due to high morbidity and mortality rates during recurrent outbreaks in endemic areas. Therefore, the development of countermeasures against priority, requires availability appropriate animal models for efficacy evaluations. The most commonly used nonhuman primate evaluations utilize intramuscular or aerosol route exposure. Although clinical disease signs are similar human cases, progression these is much more rapid, this can pose...
Abstract It is well established that cancers are heterogeneous diseases with biological, genetic, and histopathological differences among patients within individual tumors. Consequently, it not surprising animal models based on cell lines their clonal nature adaptations to defined media have limited preclinical value. Recent advances in the generation of patient-derived xenografts (PDX), where patient material engrafted into immunosuppressed mice, suggests this approach may provide a more...
Purpose: Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne with wide geographical distribution, which includes Africa, the Balkans, Middle East, Russia and western Asia. As result of its dissemination, there considerable amount genetic diversity amongst different CCHFV strains. Infection can cause severe, often fatal, human disease characterized by hemorrhage. No licensed vaccines or therapeutics currently exist to treat infection CCHFV. The development non-human primate (NHP)...
ABSTRACT Most alphaviruses are mosquito-borne and can cause severe disease in humans domesticated animals. In North America, eastern equine encephalitis virus (EEEV) is an important human pathogen with case fatality rates of 30-90%. Currently, there no therapeutics or vaccines to treat and/or prevent infection. One critical impediment countermeasure development the lack insight into clinically relevant parameters a susceptible animal model. This study examined course EEEV cynomolgus macaque...