A5030661028- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Protein purification and stability
- Hemoglobin structure and function
- Erythrocyte Function and Pathophysiology
- Histiocytic Disorders and Treatments
- Galectins and Cancer Biology
- Nanoparticle-Based Drug Delivery
- Chronic Lymphocytic Leukemia Research
- Advanced Breast Cancer Therapies
- Organometallic Complex Synthesis and Catalysis
- Acute Myeloid Leukemia Research
- Growth Hormone and Insulin-like Growth Factors
- Cell Adhesion Molecules Research
- Calcium Carbonate Crystallization and Inhibition
- Tuberous Sclerosis Complex Research
- Immunotherapy and Immune Responses
- Multiple Myeloma Research and Treatments
- Dendrimers and Hyperbranched Polymers
Ablynx (Belgium)
2022
VIB-UGent Center for Inflammation Research
2021
Ghent University
2013-2021
Abstract Binding to the neonatal Fc receptor (FcRn) extends serum half-life of IgG, and antagonizing this interaction is a promising therapeutic approach in IgG-mediated autoimmune diseases. Fc-MST-HN, designed for enhanced FcRn binding capacity, has not been evaluated context full-length antibody, structural properties attached Fab regions might affect FcRn-mediated intracellular trafficking pathway. Here we present comprehensive comparative analysis IgG salvage pathway between two...
The therapeutic scope of antibody and nonantibody protein scaffolds is still prohibitively limited against intracellular drug targets. Here, we demonstrate that the Alphabody scaffold can be engineered into a cell-penetrating antagonist induced myeloid leukemia cell differentiation MCL-1, an target in cancer, by grafting critical B-cell lymphoma 2 homology 3 helix MCL-1 onto tagging scaffold's termini with designed cell-penetration polypeptides. Introduction albumin-binding moiety extended...
The overarching paradigm for the activation of class III and V receptor tyrosine kinases (RTKs) prescribes cytokine-mediated dimerization ectodomains homotypic receptor–receptor interactions. However, structural studies have shown that hematopoietic FLT3, a RTK, does not appear to engage in such contacts, despite its efficient by dimeric FLT3 ligand (FL). As part efforts better understand intricacies activation, we sought engineer monomeric FL. It was found Leu27Asp substitution at dimer...
Hybrid approaches in structural biology have had a tremendous impact on our ability to tackle complex biological problems including large and flexible protein-protein assemblies. We been employing creative combinations of X-ray crystallography, Small-angle Scattering (SAXS) electron microscopy conjunction with molecular interaction studies cellular interrogation the systems under study elucidate mechanistic principles underlying diverse cytokine-receptor Our revealed unexpected diversity...