A5064825770- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- T-cell and B-cell Immunology
- Antimicrobial Resistance in Staphylococcus
- Immunotherapy and Immune Responses
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotics Pharmacokinetics and Efficacy
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Bacillus and Francisella bacterial research
- Glaucoma and retinal disorders
- Nanowire Synthesis and Applications
- bioluminescence and chemiluminescence research
- Advanced Fluorescence Microscopy Techniques
- Mass Spectrometry Techniques and Applications
- Yersinia bacterium, plague, ectoparasites research
- Photochromic and Fluorescence Chemistry
Xi'an Jiaotong University
2011-2025
University of Electronic Science and Technology of China
2025
Chengdu Women's and Children's Central Hospital
2025
First Affiliated Hospital of Xi'an Jiaotong University
2020-2024
Lanzhou University
2023-2024
Lanzhou University Second Hospital
2023-2024
Tongji University
2023-2024
Shandong University of Traditional Chinese Medicine
2024
Shandong Shierming Eye Hospital
2013-2024
University of Alberta
2017-2023
Abstract l -Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles shuttling -lactate, we now report intensiometric green fluorescent genetically encoded biosensor for extracellular -lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging -lactate cultured mammalian cells and brain tissue.
To investigate the mechanisms of tigecycline resistance in isogenic Acinetobacter baumannii isolate pairs as well 65 unique clinical A. isolates obtained during MagicBullet trial from Greece, Italy and Spain. were subjected to WGS regulatory genes resistance-nodulation-cell division (RND)-type efflux pumps analysed. MICs determined by agar dilution expression RND-type was measured semi-quantitative RT–PCR. In pairs, disruption adeS or adeN ISs increased adeB adeJ conferred at least three...
Abstract Protein scaffolds can provide a promising alternative to antibodies for various biomedical and biotechnological applications, including therapeutics. Here we describe the design development of Alphabody, protein scaffold featuring single-chain antiparallel triple-helix coiled-coil fold. We report affinity-matured Alphabodies with favourable physicochemical properties that specifically neutralize human interleukin (IL)-23, pivotal therapeutic target in autoimmune inflammatory...
Motivated by the growing recognition of citrate as a central metabolite in variety biological processes associated with healthy and diseased cellular states, we have developed series high-performance genetically encoded biosensors suitable for imaging concentrations mammalian cells. The design these was guided structural studies citrate-responsive sensor histidine kinase took advantage same conformational changes proposed to propagate from binding domain catalytic domain. Following extensive...
Potassium ion (K + ) plays a critical role as an essential electrolyte in all biological systems. Genetically-encoded fluorescent K biosensors are promising tools to further improve our understanding of -dependent processes under normal and pathological conditions. Here, we report the crystal structure previously reported genetically-encoded biosensor, GINKO1, -bound state. Using structure-guided optimization directed evolution, have engineered improved designated GINKO2, with higher...
Abstract Nearly all bacteria exhibit a type of phenotypic growth described as persistence that is thought to underlie antibiotic tolerance and recalcitrant chronic infections. The chromosomally encoded high-persistence (Hip) toxin–antitoxin proteins HipASO HipBSO from Shewanella oneidensis, proteobacterium with unusual respiratory capacities, constitute II protein module. Here we show phosphorylated can engage in an unexpected ternary complex double-stranded operator DNA distinct the...
Photocleavable proteins can enable the light-dependent modulation of biomolecular activities with high spatiotemporal precision.
DUSP6 functions as an important negative feedback component of the MAPK/ERK signaling pathway. Although expression is tightly regulated by ERK1/2 signaling, molecular mechanism this regulation remains partially understood. In work, we show that transcriptional repressor CIC downstream to negatively regulate expression. directly represses transcription binding three cis-regulatory elements (CREs) in promoter. p90RSK, a target ERK1/2, phosphorylates at S173 and S301 sites, which creates 14-3-3...
The rare metal element molybdenum functions as a cofactor in molybdoenzymes that are essential to life almost all living things. Molybdate can be captured by the periplasmic substrate-binding protein ModA of ModABC transport system bacteria. We demonstrate plays crucial roles growth, multiple metabolic pathways, and ROS tolerance Acinetobacter baumannii . Crystal structures molybdate-coordinated A. show noncanonical disulfide bond with conformational change between reduced oxidized states....
The type II secretion system is a multiprotein assembly spanning the inner and outer membranes in Gram-negative bacteria. It found almost all pathogenic bacteria where it contributes to virulence, host tissue colonization, infection. exoproteins are secreted across membrane via large translocation channel, secretin, which typically adopts dodecameric structure. These secretin channels have periplasmic N-terminal domains that reach out into periplasm for communication with platform...
AdeR-AdeS is a two-component regulatory system, which controls expression of the adeABC efflux pump involved in Acinetobacter baumannii multidrug resistance. AdeR response regulator consisting an N-terminal receiver domain and C-terminal DNA-binding-domain. binds to direct-repeat DNA intercistronic region between adeR adeABC. We demonstrate markedly high affinity binding unphosphorylated with dissociation constant 20 nM. In addition, we provide 2.75 Å crystal structure DNA-binding-domain...
Acinetobacter baumannii can develop various resistance mechanisms to carbapenems. However, the factors involved in evolutionary process that leads from transition sensitive resistant phenotype are not clear.
Enterotoxigenic Bacteroides fragilis (ETBF) can rapidly secrete an enterotoxin termed B. toxin (BFT), which is thought to be the only recognized virulence factor in ETBF. ETBF cause acute diarrhea, inflammatory bowel disease (IBD), colorectal cancer, and breast cancer. BFT divided into three subtypes, BFT1, BFT2, BFT3. BFT1 most widely distributed human isolates. used as a biomarker for predicting inflammation-cancer transformation of intestine breast. Nanobodies have advantages small...