A5057217778- MicroRNA in disease regulation
- Circular RNAs in diseases
- Prostate Cancer Treatment and Research
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- Renal cell carcinoma treatment
- COVID-19 Clinical Research Studies
- Telomeres, Telomerase, and Senescence
- Prostate Cancer Diagnosis and Treatment
- Memory Processes and Influences
- Renal function and acid-base balance
- Vitamin C and Antioxidants Research
- Cancer, Stress, Anesthesia, and Immune Response
- FOXO transcription factor regulation
- Gout, Hyperuricemia, Uric Acid
- Cancer-related Molecular Pathways
- Estrogen and related hormone effects
- Protease and Inhibitor Mechanisms
- Bladder and Urothelial Cancer Treatments
- Long-Term Effects of COVID-19
- RNA Research and Splicing
- S100 Proteins and Annexins
- Dementia and Cognitive Impairment Research
- Extracellular vesicles in disease
- Cognitive Functions and Memory
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2015-2025
Universidade de São Paulo
2015-2024
COVID-19 represents a public health emergency, whose mechanism of which is not fully understood. It speculated that microRNAs may play crucial role in host cells after infection by SARS-CoV-2. Thus, our study aimed to analyze the expression miR-200c-3p saliva samples from patients with COVID-19. One handred eleven were divided into 4 groups. Group I: 39 negative for Covid-19; II: 37 positive and symptomatic patients, no indication hospitalization; III: 21 respiratory disorders...
Novel biomarkers are needed to complement prostate specific antigen (PSA) in cancer (PCa) diagnostic screening programs. Glycoproteins represent a hitherto largely untapped resource with great potential as and sensitive tumor due their abundance bodily fluids dynamic cancer-associated glycosylation. However, quantitative glycoproteomics strategies detect glycoprotein markers from complex biospecimen only just emerging. Here, we describe strategy for deep mapping of N- O-glycoproteins urine...
The acquisition of a castration-resistant prostate cancer phenotype by cells is the alteration that has worst prognosis for patients. aim this study was to evaluate role microRNAs-23b/-27b as well possible CCNG1 target gene in tissue samples from patients with localized progressed and cell line (PC-3). microRNAs expression levels surgical specimens were analyzed quantitative real-time polymerase chain reaction. line, PC-3, transfected pre-miR-23b, pre-miR-27b, their respective controls using...
Prostate cancer (PCa) has a high prevalence and represents an important health problem, with increased risk of metastasis. With the advance CRISPR-Cas9 genome editing, new possibilities have been created for investigating PCa. The technique is effective in knockout oncogenes, reducing tumor resistance. MMP9 miR-21 target genes are associated PCa progression; therefore, we evaluated MMP-9 targets using system. Single guide RNAs (sgRNAs) sequences were inserted into PX-330 plasmid, transfected...
Clear cell renal carcinoma (ccRCC) is the third most common urological cancer in adults. Our aim to evaluate genes and miRNAs expression profiles involved with angiogenesis tumor characteristics ccRCC. The levels of miR-99a, 99b, 100; 199a; 106a; 106b; 29a; 29b; 29c; 126; 200a, 200b their respective target genes: mTOR, HIF1-α, VHL, PDGF, VEGF, VEGFR1 VEGFR2 were analyzed using qRT-PCR tissue samples from 56 patients Five benign utilized as control. related size, Fuhrman nuclear grade...
Phospholipase A2 (PLA2) is a large family of enzymes involved in the inflammatory process that catalyzes hydrolysis membrane phospholipids, leading to production free fatty acids and lysophospholipids, starting arachidonic acid cascade. Their expression has been related behavior several cancers. Our objective search for PLA2 prostate cancer (PCa) tissue correlates with prognosis survival.
Introduction and objective: Overexpression of MMPs has been related to biochemical recurrence after radical prostatectomy. TIMP1 TIMP2 are controllers the aim this study is evaluate expression levels their regulators using immunohistochemistry in tissue microarray localized prostate cancer (PC). Materials Methods: Immune-expression MMP-9, MMP-2, TIMP1, TIMP-2, MMP-14 IL8, were analyzed by prostatectomy specimens 40 patients with PC who underwent surgery between September 1997 February 2000....
BACKGROUND/AIMS: Cholesterol modulates intratumoral androgenic signaling in prostate cancer; however, the molecular mechanisms underlying these changes castration-resistant cancer (CRPC) are not fully elucidated. Herein, we investigated effect of cholesterol on androgen receptor (AR) coactivators expression and tumorigenesis vitro vivo. METHODS: monitored AR (SRC-1, 2, 3 PCAF) genes PC-3 cells exposed to 2µg/mL for 8 hours by qPCR. We also performed cell migration at 0, 8, 24, 48 72h flow...
MicroRNAs (miRNAs) have gained a prominent role as biomarkers in prostate cancer (PCa). Our study aimed to evaluate the potential suppressive effect of miR-137 model advanced PCa with and without diet-induced hypercholesterolemia. In vitro, PC-3 cells were treated 50 pmol mimic for 24 h, gene protein expression levels SRC-1, SRC-2, SRC-3, AR evaluated by qPCR immunofluorescence. We also assessed migration rate, invasion, colony-forming ability, flow cytometry assays (apoptosis cell cycle)...
Peyronie's disease (PD) is characterized by the formation of fibrous plaque in tunica albuginea, causing several problems patients. The etiology this not fully understood, and there are few effective treatments. To better understand molecular pathways PD, we studied miR-29b, a microRNA that could be involved with illness. MicroRNAs endogenous molecules act inhibiting messenger RNA. MiR-29b regulates 11 20 collagen genes TGF-β1 gene, which related to PD progression.We compared miR-29b...
Prostate cancer has a high incidence in men and is the second cause of death among americans male. microRNA (miR) becoming potential new prognostic factor for prostate cancer. Single nucleotide polymorphisms (SNPs) are common polymorphisms, characterized by single exchange nitrogen based DNA. This polymorphism present miRs, altering their function.To evaluate role SNP rs1834306 miR100 rs2910164 miR146a development prognosis cancer.One hundred patients diagnosed with 68 controls were...
Radical prostatectomy, a standard management approach for localized Prostate Cancer (PC), may cause stress response associated with immune modulating effects. Regional anesthesia was hypothesized to reduce the effects of surgery by minimizing neuroendocrine surgical response, thus mitigating tumor cells dissemination. Our primary objective investigate whether use spinal blocks attenuates PC dissemination on an animal model. We also assessed number circulating NK and amount inflammatory...
Abstract Background Telomere dysfunction is one of the hallmarks cancer and crucial to prostate carcinogenesis. TERF1 a gene essential telomere maintenance, its has already been associates with several cancers. target miR-155, this microRNA can inhibit expression promotes carcinogenesis in breast cancer. We aim analyze TERF1, mRNA level, involvement progression. Results Alterations DNA were evaluated using datasets primary tumor castration-resistant tumors (CRPC) deposited cBioportal. The...
Prostate cancer is the most frequent malignancy affecting men worldwide. Due to low sensitivity and specificity of prostate-specific antigen test digital rectal exam as screening modalities, several alternatives are being studied. This study aimed evaluate application MMP-9 its regulators (TIMP-1, RECK, miR-338-3p) diagnostic prognostic indicators prostate cancer. A total 134 randomly selected patients under investigation for submitted a transrectal ultrasound-guided biopsy were enrolled in...
Previous studies have suggested that performance prediction, an aspect of metamemory, may be associated with objective on memory tasks. OBJECTIVE: The the study was to describe prediction before performing episodic task, in community-dwelling older adults, stratified by sex, age group and educational level. Additionally, association between predicted a task investigated. METHODS: based data from 359 participants FIBRA carried out at Ermelino Matarazzo, São Paulo. Memory assessed posing...
You have accessJournal of UrologyBladder and Urethra: Anatomy, Physiology Pharmacology II1 Apr 2015MP21-10 PHYSICAL ACTIVITY MAY PREVENT BLADDER DYSFUNCTION IN OBESE RATS THROUGH OVER-EXPRESSION OF INSULIN SIGNALING RELATED GENES Andre Matos Oliveira, Fernando F. Fonseca, Sabrina T. Reis, Vanessa Guimaraes, Katia R. Leite, William C. Nahas, Miguel Srougi, Alberto A. Antunes OliveiraAndre Oliveira More articles by this author , FonsecaFernando Fonseca ReisSabrina Reis GuimaraesVanessa...
You have accessJournal of UrologyKidney Cancer: Basic Research & Pathophysiology II1 Apr 2016MP85-09 ANGIOGENESIS RELATED MICRORNAS AND GENE EXPRESSION PROFILE IN RENAL CELL CARCINOMA, CLEAR TYPE Rita de Cássia Oliveira, Nayara Viana, Katia Leite, Vanessa Guimarães, José Pontes, Renato Ivanovic, Caio Moura, Denis Reis, Nelson Dip, William Nahas, Miguel Srougi, and Sabrina Reis OliveiraRita Oliveira More articles by this author , VianaNayara Viana LeiteKatia Leite GuimarãesVanessa Guimarães...
Background: Bladder Cancer (BCa) is the tenth most common type of cancer, presenting significant morbidity, mortality, and healthcare costs, especially in more advanced cases. New biomarkers disease mechanisms oncology have been studied, among them matrix metalloproteinases (MMPs) their role cancer progression metastasis. Therefore, we analyzed MMP-9 gene expression downregulation effects by CRISPR-Cas9 genome editing technique T24-luc bladder urothelial carcinoma cell lines.Materials...