A5064774995- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Parkinson's Disease Mechanisms and Treatments
- Forensic Toxicology and Drug Analysis
- Neurobiology and Insect Physiology Research
- Botulinum Toxin and Related Neurological Disorders
- Phytochemistry and biological activities of Ficus species
- Ion channel regulation and function
- Pesticide Exposure and Toxicity
- Alzheimer's disease research and treatments
- Lipid Membrane Structure and Behavior
- Pharmacological Effects and Assays
- Nerve injury and regeneration
- Receptor Mechanisms and Signaling
- Lysosomal Storage Disorders Research
- Crystallization and Solubility Studies
- Mosquito-borne diseases and control
- Cannabis and Cannabinoid Research
- Alcohol Consumption and Health Effects
- Bipolar Disorder and Treatment
- Insect Resistance and Genetics
- Drug Solubulity and Delivery Systems
- Pancreatic function and diabetes
- Neurological disorders and treatments
- Metabolomics and Mass Spectrometry Studies
University of Cagliari
2000-2024
Istituto di Farmacologia Traslazionale
2018-2024
Sardegna Ricerche (Italy)
1999-2023
National Research Council
2018-2023
Parkinson's disease (PD) diagnosis is still vulnerable to bias, and a definitive often relies on post-mortem neuropathological diagnosis. In this regard, alpha-synuclein (αsyn)-specific in vivo biomarkers remain critical unmet need, based its relevance the neuropathology. Specifically, content changes αsyn species such as total (tot-αsyn), oligomeric (o-αsyn), phosphorylated (p-αsyn) within cerebrospinal fluid (CSF) peripheral fluids (i.e., blood saliva) have been proposed PD possibly...
Abstract Two γ‐hydroxybutyric acid (GHB) analogues, trans ‐γ‐hydroxycrotonic (t‐HCA) and γ‐(p‐methoxybenzyl)‐γ‐hydroxybutyric (NCS‐435) displaced [ 3 H]GHB from GHB receptors with the same affinity as but, unlike GHB, failed to displace H]baclofen GABA B receptors. The effect of baclofen, on G protein activity hippocampal extracellular glutamate levels was compared. While baclofen stimulated 5′‐O‐(3‐[ 35 S]thiotriphospate) S]GTPγS binding both in cortex homogenate cortical slices, t‐HCA...
The accumulation of aggregated α-synuclein (αSyn) is a hallmark Parkinson's disease (PD). Current evidence indicates that small soluble αSyn oligomers (αSynOs) are the most toxic species among forms aggregates, and size topological structural properties crucial factors for αSynOs-mediated toxicity, involving interaction with either neurons or glial cells. We previously characterized human αSynO (H-αSynO) specific promoting toxicity against neuronal membranes. Here, we tested neurotoxic...
Dithiocarbamate compounds are widely used agricultural fungicides that display low acute toxicity in mammals and may become neurotoxic after prolonged exposure. Mancozeb, among other dithiocarbamates tested, proved to be the most potent (Ki= 0.27 microM) at noncompetitively inhibiting vitro ATP-dependent uptake of [3H]glutamate rat cortical vesicles. Furthermore, mancozeb partially (20%) inhibited [14C]methylamine, as an index for vesicular transmembrane proton gradient (DeltapH), evoked its...
The search for new disease-modifying drugs Parkinson's disease is a slow and highly expensive process, the repurposing of already approved different medical indications becoming compelling alternative option researchers. Genetic variables represent predisposing factor to mutations in Leucine-rich repeat kinase 2 (LRRK2) locus have been correlated late-onset autosomal-dominant PD. common fruit fly Drosophila melanogaster carrying mutation LRRK2 loss-of-function WD40 domain (LRRK2WD40), simple...
Intoxication with the alcohol-aversive drug disulfiram (Antabuse) and related dithiocarbamates may provoke neuropathies and, in some cases, damage basal ganglia. Rats received a single administration of (7 500 mg kg-1 i.p.) equimolar doses (4 290 its metabolite diethyldithiocarbamate (DDC), roughly corresponding to daily maximum dose alcohol abusers or an estimated nonlethal overdose, respectively. The striatal, extracellular levels glutamate freely moving rats previously implanted...
Parkinson’s disease (PD) is a neurodegenerative disorder characterized by the loss of dopaminergic neurons responsible for unintended or uncontrollable movements. Mutations in leucine-rich repeat kinase 2 locus contribute to genetic forms PD. The fruit fly Drosophila melanogaster carrying this mutation (LRRK2-Dm) an vivo model PD that develops motor impairment and stands eligible non-mammalian paradigm test novel therapeutic approaches. Dehydrozingerone (DHZ) natural phenolic compound...
Abstract Introduction Increased glutamate levels and electrolytic fluctuations have been observed in acutely manic patients. Despite some efficacy of the non‐competitive NMDA receptor antagonist memantine (Mem), such as antidepressant‐like mood‐stabilizer drugs clinical studies, its specific mechanisms action are still uncertain. The present study aims to better characterize Drosophila melanogaster fly Shaker mutants (SH), a translational model episodes within bipolar disorder humans,...
Within neurodegenerative syndromes, Parkinson's disease (PD) is typically associated with its locomotor defects, sleep disturbances and related dopaminergic (DA) neuron loss. The fruit fly, Drosophila melanogaster (D. melanogaster), leucine-rich repeat kinase 2 mutants (LRRK2) loss-of-function in the WD40 domain, provides mechanistic insights into corresponding human behaviour, possibly disclosing some physiopathologic features of PD both genetic sporadic forms. Moreover, several data...
Parkinson's disease (PD) is a neurodegenerative disorder characterized by the loss of dopamin-ergic neurons responsible for unintended or uncontrollable movements. Mutations in leu-cine-rich repeat kinase 2 locus, contributes to genetic forms PD. The fruit fly Drosophila mela-nogaster carrying this mutation (LRRK2-Dm), an vivo model PD that develops motor impairment and stands eligible non mammalian paradigm test novel therapeutic ap-proaches. Dehydrozingerone (DHZ) natural phenolic...
We aimed to ascertain whether pyrethroid insecticides could influence the vesicular transport of excitatory amino acid glutamate. The incubation rat cortical synaptic vesicles with resmethrin and permethrin, consistently stimulated both ATP-dependent -independent uptake [3H]glutamate, while not evoking depletion its content. Both processes were counteracted by valinomycin, a dissipator transmembrane potential gradient (deltapsi(sv)). Meanwhile, influx 36Cl- anions was impaired concentrations...