Melatonin (MT) and nitric oxide (NO) are two multifunctional signaling molecules that involved in the response of plants to abiotic stresses. However, how MT NO synergize cold stress affecting is still not clear. In this study, we found endogenous accumulation under was positively correlated with tolerance different varieties cucumber seedlings. The data presented here also provide evidence stress. About 100 μM significantly increased nitrate reductase (NR) activity, NR -relative messenger...

In northern China, low temperature is the most common abiotic stresses for tomato plants cultivated in solar-greenhouse winter. We recently found that expression and enzyme activity of fructose-1,6-bisphosphate aldolases (FBAs) tomato, which are important enzymes Calvin-Benson cycle (CBC), were significantly altered seedlings subjected to heat/cold stresses. order study role FBA photosynthesis regulating cold stress responses (Solanum lycopersicum), we transformed a inbred line (FF) with RNA...

Salicylic acid (SA) has been proven to be a multifunctional signaling molecule that participates in the response of plants abiotic stresses. In this study, we used cold-sensitive cucumber and cold-tolerant pumpkin as experimental materials examine roles SA root–shoot communication responses aerial or/and root-zone chilling stress own-root hetero-root grafted plants. The results showed ( Cm ) rootstock enhanced tolerance cucumber, evidenced by observed lower levels electrolyte leakage (EL),...

Rubisco activase (RCA) is an important enzyme that can catalyze the carboxylation and oxygenation activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), which involved in photosynthetic carbon reduction cycle. Here, we studied effects changes RCA activity on photosynthesis, growth development, as well low temperature weak light tolerance overexpressing transgenic cucumber (Cucumis sativus) plants. CsRCA overexpression increased plant height, leaf area dry matter, decreased...

As an important gas signaling molecule, hydrogen sulfide (H2S) plays a crucial role in regulating cold tolerance. H2S cooperates with phytohormones such as abscisic acid, ethylene, and salicylic acid to regulate the plant stress response. However, synergistic regulation of auxin response has not been reported. This study showed that sodium hydrosulfide (NaHS, donor) treatment enhanced tolerance cucumber seedlings increased level auxin. CsARF5, factor (ARF) gene, was isolated, its...

Chilling adversely affects the photosynthesis of thermophilic plants, which further leads to a decline in growth and yield. The role melatonin (MT) stress response plants has been investigated, while mechanisms by MT regulates chilling tolerance chilling-sensitive cucumber remain unclear. This study demonstrated that positively regulated seedlings 1.0 μmol⋅L-1 was optimum concentration, injury index, electrolyte leakage (EL), malondialdehyde (MDA) were lowest, highest among all treatments....

Salicylic acid (SA) plays a role in the regulation of grafting-induced cold tolerance. However, molecular mechanism behind it is still unknown. Here, we established that phenylalanine ammonia-lyase (PAL) pathway-dependent elevate SA content grafted cucumber leaves was not only synthesized but also transported from roots under chilling stress. RNAi-CsPAL with low as rootstock reduced accumulation seedling while decreasing rootstock-induced tolerance, evidenced by higher electrolyte leakage...

Melatonin (MT), as a signaling molecule, plays vital role in regulating leaf senescence plants. This study aimed to verify the antioxidant roles of MT delaying dark- or age-induced cucumber The results showed that endogenous responds darkness and overexpression CsASMT, key gene synthesis, delays stimulated by darkness, manifested significantly lower malonaldehyde (MDA) reactive oxygen species (ROS) contents well higher activities expression enzymes compared control. Moreover, suppressed both...

Drought stress is one of the main environmental factors limiting plant growth and productivity many crops. Elevated carbon dioxide concentration (eCO2) can ameliorate, mitigate, or compensate for negative impact drought on enable plants to remain turgid functional a longer period. In order investigate combined effects eCO2 photosynthetic performance leaf structures, we analyzed characteristics structure ultrastructure cucumber leaves. The decline in net rate under moderate occurred due...

Hydrogen sulfide (H2S) plays a crucial role in regulating chilling tolerance. However, the of hydrogen peroxide (H2O2) and auxin H2S-induced signal transduction stress response plants was unclear. In this study, 1.0 mM exogenous H2O2 75 μM indole-3-acetic acid (IAA) significantly improved tolerance cucumber seedlings, as demonstrated by mild plant injury symptoms, lower index (CI), electrolyte leakage (EL), malondialdehyde content (MDA) well higher levels photosynthesis cold-responsive genes...

Grafting is widely applied to enhance the tolerance of some vegetables biotic and abiotic stress. Salicylic acid (SA) known be involved in grafting-induced chilling cucumber. Here, we revealed that grafting with pumpkin (Cucurbita moschata, Cm) as a rootstock improved increased accumulation SA, abscisic (ABA) hydrogen peroxide (H2O2) grafted cucumber (Cucumis sativus/Cucurbita Cs/Cm) leaves. Exogenous SA ABA H2O2 mRNA abundances CBF1, COR47, NCED, RBOH1. However, 2-aminoindan-2-phosphonic...

Chilling causes a significant decline in photosynthesis tomato plants. Tomato tryptophan decarboxylase gene 1 (SlTDC1) is the first rate-limiting for melatonin (MT) biosynthesis and involved regulation of under various abiotic stresses. However, it not clear whether SlTDC1 participates chilling stress. Here, we obtained overexpression transgenic seedlings, which showed higher mRNA abundance MT content compared with wild type (WT). The results that obviously alleviated damage to seedlings...

Root rot caused by Fusarium solani, is one of the most severe diseases in pepper (Capsicum annuum L.). Grafting has been attempted as an effective means to control disease, but little known about disease resistance mechanism grafted pepper. Therefore, we investigated changes biomass, cell structure, and secondary metabolism roots (non-grafted pepper) peppers using cvs. Weishi Buyeding rootstocks cv. Xinfeng 2 a scion. After manual inoculation, less F. solani invaded consequently serious...