Bee pollen has been used as an apitherapy agent for several centuries to treat burns, wounds, gastrointestinal disorders, and various other diseases. The aim of our study was investigate the hepatoprotective effects chestnut bee against carbon tetrachloride (CCI4)-induced liver damage. Total phenolic content, flavonoid, ferric reducing/antioxidant power, DPPH radical activity measurements were antioxidant capacity determinants pollen. conducted in rats seven groups. Two different...

Our objective was to identify the antioxidant properties of honeybee products from Turkey, chestnut honey, pollen, propolis, and royal jelly, their hepatoprotective activity against CCl4-induced hepatic damage in rats.Animals were fed with for 7 days following CCl4 injection. Development liver oxidative stress monitored by measuring activities enzymes alanine transaminase, aspartate malondialdehyde, superoxide dismutase, catalase. Antioxidant capacities bee identified using FRAP DPPH assays,...

The periderm is a flat layer of epithelium created during embryonic development. During palatogenesis, the forms protective against premature adhesion oral epithelia, including palate. However, must be removed in order for medial edge epithelia (MEE) to properly adhere and form palatal seam. Improper removal results cleft Although timing transforming growth factor β3 (TGFβ3) expression MEE coincides with degeneration, its role desquamation not known. Interestingly, murine models knockout...

Abstract Background In humans, cleft palate (CP) accounts for one of the largest number birth defects with a complex genetic and environmental etiology. TGFβ3 has been established as an important regulator palatal fusion in mice it shown that TGFβ3-null exhibit CP without any other major deformities. However, genes regulate cellular decisions molecular mechanisms maintained by pathway throughout palatogenesis are predominantly unexplored. Our objective this study was to analyze global...

The recent observation of vector sequences in the semen men undergoing clinical gene therapy for hemophilia has highlighted need to evaluate risk inadvertent germ-line transduction a clinically relevant animal model. In present study, we used three different approaches investigate whether germ line is at alteration following utero retroviral transfer relevant, random-bred sheep First, conducted breeding studies. All organs from 10 resultant offspring were devoid proviral DNA, suggesting that...

The possibility of permanent genetic changes to the germline is central bioethics in utero gene therapy (IUGT) because concern inadvertent potentially deleterious alterations pool. Despite presumed protection male due early germ cell (GC) compartmentalization, we reported that GCs within developing ovine testes are transduced at low levels after retrovirus-mediated IUGT, thus underscoring need for a thorough understanding GC development clinically predictive models determine optimal time...

Nonsyndromic cleft palate is a common birth defect (1:700) with complex etiology involving both genetic and environmental risk factors. Nicotine, major teratogen present in tobacco products, was shown to cause alterations delays the developing fetus. To demonstrate postpartum effects of nicotine on palatal development, we delivered three different doses (1.5, 3.0, 4.5mg/kg/d) sterile saline (control) into pregnant BALB/c mice throughout their entire pregnancy using subcutaneous micro-osmotic...

Abstract During palatogenesis, the palatal mesenchyme undergoes increased cell proliferation resulting in growth, elevation and fusion of two shelves. Interestingly, expresses all three transforming growth factor (TGF) β isoforms (1, 2, 3) throughout these steps palatogenesis. However, role TGFβ promoting mesenchymal cells has never been explored. The purpose this study was to identify effect on human embryonic (HEPM) proliferation. Our results showed that TGFβ, especially TGFβ3, HEPM by...

The direct vector injection approach was used in the fetal sheep model of utero gene therapy to determine effects recipient gestational age on efficacy and pattern liver, lung, brain transduction transgene expression. livers contained foci transgene-expressing hepatocytes demonstrated an inverse correlation between hepatocyte transduction/transgene expression, with higher levels transfer/expression early gestation lower late gestation. Conversely, percentage cells within lungs these same...

Development of the palate comprises sequential stages growth, elevation, and fusion palatal shelves. The mesenchymal component palates plays a major role in early phases palatogenesis, such as growth elevation. Failure these steps may result cleft palate, second most common birth defect world. These palatogenesis require precise chronological orchestration key physiological processes, proliferation, differentiation, migration, apoptosis. There is compelling evidence for vital TGFβ-mediated...

Canola is a significant alternate oilseed crop in Southeastern Anatolia, Turkey.While, there no evidence for good yield potential canola cultivars are available this area.Therefore, the study was conducted with three consecutive two seasons (2013-14 and 2014-15) to find out appropriate which suitable grow under environmental condition of Turkey.The experiment carried randomized complete block design replications.After years observation, it found that plant height branches -1 all were varied...

A fundamental obstacle to using retroviral-mediated gene transfer (GT) treat human diseases is the relatively low transduction levels that have been achieved in clinically relevant cells. We previously showed performing GT utero overcomes this and results significant of within multiple fetal organs, with different tissues exhibiting optimal at developmental stages. undertook present study aiming elucidate mechanism for age-dependent transduction, testing two factors we hypothesized could be...

α-Tocopherol and β-carotene are the best known most widely used natural antioxidant substances. Apricot contains β-carotene, tocopherols flavonoids. This experimental study was designed to investigate protective effect of Malatya kabashi apricot in stress-induced injury various tissues rats. In total, 32 male Wistar albino rats were divided into four groups: control, apricot, stress apricot-stress groups. administrated by gavage for 10 days Then kept at 4°C 4 h The killed end experiment...

Article International Workshop on Process Intensification 2015 (IWPI2015): Towards Sustainable Technologies in the 21st Century (Canik Basari University, Samsun, Turkey, April 27–30, 2015) was published December 1, 2014 journal Green Processing and Synthesis (volume 3, issue 6).

We have recently reported that the gestational age of recipient determines pattern and level transgene expression following in utero retroviral gene transfer by direct injection into pre-immune fetal sheep (Molecular Therapy 11(2):284-293, 2005). These studies suggest developmental stage each organ at time may determine its susceptibility to transfer, possesses own unique temporal window during which optimal transduction can be achieved. In present studies, we sought mechanism recipient's...