A5108001791- Microbial Community Ecology and Physiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotic Resistance in Bacteria
- Methane Hydrates and Related Phenomena
- Genomics and Phylogenetic Studies
- Gut microbiota and health
- Diatoms and Algae Research
- Geology and Paleoclimatology Research
- Antibiotics Pharmacokinetics and Efficacy
- Marine and environmental studies
- Water Treatment and Disinfection
- Hydrocarbon exploration and reservoir analysis
- Paleontology and Stratigraphy of Fossils
- Protist diversity and phylogeny
- Animal Virus Infections Studies
- Analytical chemistry methods development
- Microbial Metabolism and Applications
- Marine and coastal ecosystems
- Spectroscopy Techniques in Biomedical and Chemical Research
- Microbial infections and disease research
- Environmental DNA in Biodiversity Studies
- Aquatic Ecosystems and Phytoplankton Dynamics
- Animal testing and alternatives
- Polar Research and Ecology
- Microbial Fuel Cells and Bioremediation
National Institute of Research and Development for Biological Sciences
2015-2024
Babeș-Bolyai University
2010-2015
Abstract When antimicrobial resistant bacteria (ARB) and genes (ARGs) reach novel habitats, they can become part of the habitat’s microbiome in long term if are able to overcome biotic resilience towards immigration. This process should more difficult with increasing biodiversity, as exploitable niches a given habitat reduced for immigrants when diverse competitors present. Consequently, microbial diversity could provide natural barrier resistance by reducing persistence time immigrating ARB...
River microbial communities regularly act as the first barrier of defense against spread antimicrobial resistance genes (ARGs) that enter environmental microbiomes through wastewater. However, how invasion dynamics wastewater-borne ARGs into river biofilm will shift due to climate change with increasing average and peak temperatures remains unknown. Here, we aimed elucidate effects on naturally occurring resistome, well success foreign entering Natural biofilms were grown in a...
For the successful treatment of infections, real-time analysis and enhanced multiplex capacity, sensitivity cost-effectiveness developed detection method are critical.
Ursu Lake is located in the Middle Miocene salt deposit of Central Romania. It stratified, and water column has three distinct masses: an upper freshwater-to-moderately saline stratum (0-3 m), intermediate exhibiting a steep halocline (3-3.5 lower hypersaline (4 m below) that euxinic (i.e. anoxic sulphidic). Recent studies have characterized lake's microbial taxonomy given rise to intriguing ecological questions. Here, we explore whether communities are dynamic or stable relation taxonomic...
This report describes the biodiversity and ecology of microbial mats developed in thermal gradients (20 to 65°C) surroundings three drillings (Chiraleu [CH], Ciocaia [CI], Mihai Bravu [MB]) tapping a hyperthermal aquifer Romania. Using metabarcoding approach, 16S rRNA genes were sequenced from both DNA RNA transcripts (cDNA) compared. The relationships between diversity physicochemical factors explored. Additionally, cDNA data used for silico functionality predictions, bringing new insights...
Abstract The aim of this paper is to describe a new variant Janthinobacterium lividum - ROICE173, isolated from Antarctic snow, and investigate the antimicrobial effect crude bacterial extract against 200 multi-drug resistant (MDR) bacteria both clinical environmental origin, displaying various antibiotic resistance patterns. ROICE173 extremotolerant, grows at high pH (5.5–9.5), in salinity (3%) presence different xenobiotic compounds antibiotics. best violacein yield (4.59 ± 0.78 mg·g −1...
Modern mineral deposits play an important role in evolutionary studies by providing clues to the formation of ancient lithified microbial communities. Here we report presence microbialite-forming mats different microenvironments at 32°C, 49°C, and 65°C around geothermal spring from abandoned oil drill Ciocaia, Romania. The mineralogy macro- microstructure microbialites were investigated, together with their diversity based on a 16S rRNA gene amplicon sequencing approach. calcium carbonate is...
Abstract A mud volcano (MV) is a naturally hydrocarbon-spiked environment, as indicated by the presence of various quantities PAHs and aromatic isotopic shifts in its sediments. Recurrent expulsion hydrocarbons consolidates growth hydrocarbonoclastic bacterial communities areas around MVs. In addition to widely-known availability biologically malleable alkanes, MVs can represent hotbeds polyaromatic (PAHs), well - an aspect that has not been previously explored. This study measured highly...
The spatiotemporal variation of several carbapenemase-encoding genes (CRGs) was investigated in the influent and effluent municipal WWTPs, with or without hospital sewage input. Correlations among gene abundances, bacterial community composition, wastewater quality parameters were tested to identify possible predictors CRGs presence. Also, role wastewaters mirroring clinical resistance is discussed. taxonomic groups abundances showed an even distribution types, meaning that does not...
Abstract Background In the environment, microbial communities are constantly exposed to invasion by antimicrobial resistant bacteria (ARB) and their associated resistance genes (ARGs) that were enriched in anthroposphere. A successful invader has overcome biotic resilience of habitat, which is more difficult with increasing biodiversity. The capacity exploit resources a given habitat enhanced when exhibit greater diversity, reducing opportunities for invaders, leading lower persistence....
A high-performance liquid chromatography method is reported for the determination of antibiotics in water. The were simultaneously preconcentrated by solid-phase extraction. High-performance was performed on a C18 modified column with gradient elution 25 min at 40°C. separation using 90:10 acetonitrile:water and 0.1% aqueous formic acid. identified diode array detection mass spectrometry. established suitable surface
Carbapenemase-producing Klebsiella pneumoniae (CPKP) isolated from influent (I) and effluent (E) of two wastewater treatment plants, with (S1) or without (S2) hospital contribution, were investigated. The strains belonged to the Kp1 phylogroup, their highest frequency being observed in S1, followed by S2. phenotypic genotypic hypervirulence tests negative for all tested. At least one carbapenemase gene (CRG), belonging blaKPC, blaOXA-48, blaNDM blaVIM families, was 63% CPKP, more than half...
Present-day terrestrial analogue sites are crucial ground truth proxies for studying life in geochemical conditions close to those assumed be present on early Earth or inferred exist other celestial bodies (e.g. Mars, Europa). Although hypersaline sapropels border-of-life habitats with moderate occurrence, their microbiological and physicochemical characterization lags behind. Here, we study the diversity of under low water activity by describing prokaryotic communities from two disparate...
As a consequence of global demographic challenges, both the artificial and natural environment are increasingly impacted by contaminants emerging concern, such as bacterial pathogens their antibiotic resistance genes (ARGs). The aim this study was to determine extent which anthropogenic contamination contributes spread resistant enterococci in aquatic compartments explore genetic relationships among Enterococcus strains. Antimicrobial susceptibility testing (ampicillin, imipenem,...
Numerous sections of the Mureş River vary in terms abundance nitrates, ammonia, and orthophosphates; correlated lotic sediment bacterial microbiome structures both diversity abundance. This highlights great versatility microbiomes being influenced by physical-chemical characteristics environments their spatial changes. Bacteria exhibit dynamic shifting potential significant tendencies toward self-organization self-adaptation. These typical features represent an essential ecologic basis for...
Abstract Background and aims Mercury (Hg) contamination poses severe human environmental health risks. We aimed to evaluate the colonization of Hg-contaminated sites by native plants prokaryotic composition rhizosphere soil communities dominant plant species. Methods A field study was conducted at a site in Romania. Metal concentrations samples were analyzed using portable X-ray fluorescence spectrometry. The determined through 16S rRNA amplicon sequencing community functionality predicted...